Actin cytoskeleton in mesenchymal-to-amoeboid transition of cancer cells

Alexandrova, Antonina Y.; Chikina, Aleksandra; Svitkina, Tatyana

doi:10.1016/bs.ircmb.2020.06.002

Cited by 33 publications

(22 citation statements)

References 280 publications

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“…Studies of Walker 256 breast carcinosarcoma cell migration on micropatterned surfaces demonstrated that when cells moved from an adhesive to a non-adhesive substrate, lamellipodia could change to blebs and vice versa [216]. HT1080 fibrosarcoma cells cultured on a 2D adhesive substrate preferentially exhibited mesenchymal phenotype but on a substrate with reduced adhesiveness, a part of the cell population switched to forming blebs [217]. Cells switch to amoeboid migration under conditions of physical confinement and decreased adhesion [218].…”

Section: Mesenchymal-ameboid Transition (Mat)mentioning

confidence: 99%

Phenotypic Plasticity of Cancer Cells Based on Remodeling of the Actin Cytoskeleton and Adhesive Structures

Rubtsova¹,

Zhitnyak²,

Gloushankova³

2021

IJMS

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There is ample evidence that, instead of a binary switch, epithelial-mesenchymal transition (EMT) in cancer results in a flexible array of phenotypes, each one uniquely suited to a stage in the invasion-metastasis cascade. The phenotypic plasticity of epithelium-derived cancer cells gives them an edge in surviving and thriving in alien environments. This review describes in detail the actin cytoskeleton and E-cadherin-based adherens junction rearrangements that cancer cells need to implement in order to achieve the advantageous epithelial/mesenchymal phenotype and plasticity of migratory phenotypes that can arise from partial EMT.

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Section: Mesenchymal-ameboid Transition (Mat)mentioning

confidence: 99%

Phenotypic Plasticity of Cancer Cells Based on Remodeling of the Actin Cytoskeleton and Adhesive Structures

Rubtsova¹,

Zhitnyak²,

Gloushankova³

2021

IJMS

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“…Different from the sandwich method, our device uses stacked semi-transparent agarose gel to apply compression force, so besides easy cell harvesting it can also allow for real-time observation of the dynamic morphological change and cell migration behaviour of the cells during compression experiments. Increasing evidence indicates that compression force induces cancer cells to alter their morphology and protein expressions to allow them to retain their migration capability in different environmental challenges ( Fort et al, 2018 ; Holle et al, 2019 ; Alexandrova et al, 2020 ). Although the plasticity of cellular membrane protrusions is known to be involved in cancer cell migration and metastasis, how compression force influences these protrusions on a low-cell-adhesion surface is unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Although the plasticity of cellular membrane protrusions is known to be involved in cancer cell migration and metastasis, how compression force influences these protrusions on a low-cell-adhesion surface is unclear. Our device provides a new tool to study cancer cell protrusion changes under compression force in a low cell-substrate adhesion condition may help gain new insights into cancer metastasis and potentially lead to new treatment strategies ( Broders-Bondon et al, 2018 ; Fort et al, 2018 ; Alexandrova et al, 2020 ). In addition, our device may also be used for compression experiments on adhesive surfaces with a small modification; for example, seeding the cells on the glass substrate of the commercial 35 mm µ-Dishes instead of on the gel substrate.…”

Section: Discussionmentioning

confidence: 99%

A Portable Controllable Compressive Stress Device to Monitor Human Breast Cancer Cell Protrusions at Single-Cell Resolution

Yeh

Juang

Chen

et al. 2022

Front. Bioeng. Biotechnol.

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In vitro devices offer more numerous methods than in vivo models to investigate how cells respond to pressure stress and quantify those responses. Several in vitro devices have been developed to study the cell response to compression force. However, they are unable to observe morphological changes of cells in real-time. There is also a concern about cell damage during the process of harvesting cells from 3D gels. Here we report a device employing transparent, thin gel layers to clamp cells between the interfaces and applied a controllable compression force by stacking multiple layers on the top. In this approach, cells can be monitored for alteration of cellular protrusions, whose diversity has been proven to promote cancer cell dissemination, with single-cell resolution under compression force. Furthermore, p-Rac-1 and rhodamine staining on the device directly to confirm the actin filaments of lamellipodia. The method was able to fulfill real-time live-cell observation at single-cell resolution and can be readily used for versatile cell analysis. MDA-MB-231 and MCF7 breast cancer cells were utilized to demonstrate the utility of the device, and the results showed that the stimuli of compression force induce MDA-MB-231 and MCF7 to form lamellipodia and bleb protrusions, respectively. We envision the device may be used as a tool to explore mechanisms of membrane protrusion transitions and to screen drug candidates for inhibiting cancer cell protrusion plasticity for cancer therapy.

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“…During their starvation-induced aggregation, the amoebae adhere to each other and move in multicellular streams reminiscent of collective cell movements during animal embryogenesis [ 10 , 11 , 12 ]. The amoeboid locomotion of Dictyostelium cells and their capability to internalize nutrients by macropinocytosis very closely match the corresponding acquired traits of cancer cells [ 13 , 14 , 15 , 16 , 17 , 18 ] and, more generally, mirror the mesenchymal-amoeboid transition occurring during embryonal development [ 13 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 85%

Regulation of the Actin Cytoskeleton via Rho GTPase Signalling in Dictyostelium and Mammalian Cells: A Parallel Slalom

Filić

Mijanović

Putar

et al. 2021

Cells

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Both Dictyostelium amoebae and mammalian cells are endowed with an elaborate actin cytoskeleton that enables them to perform a multitude of tasks essential for survival. Although these organisms diverged more than a billion years ago, their cells share the capability of chemotactic migration, large-scale endocytosis, binary division effected by actomyosin contraction, and various types of adhesions to other cells and to the extracellular environment. The composition and dynamics of the transient actin-based structures that are engaged in these processes are also astonishingly similar in these evolutionary distant organisms. The question arises whether this remarkable resemblance in the cellular motility hardware is accompanied by a similar correspondence in matching software, the signalling networks that govern the assembly of the actin cytoskeleton. Small GTPases from the Rho family play pivotal roles in the control of the actin cytoskeleton dynamics. Indicatively, Dictyostelium matches mammals in the number of these proteins. We give an overview of the Rho signalling pathways that regulate the actin dynamics in Dictyostelium and compare them with similar signalling networks in mammals. We also provide a phylogeny of Rho GTPases in Amoebozoa, which shows a variability of the Rho inventories across different clades found also in Metazoa.

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Actin cytoskeleton in mesenchymal-to-amoeboid transition of cancer cells

Cited by 33 publications

References 280 publications

Phenotypic Plasticity of Cancer Cells Based on Remodeling of the Actin Cytoskeleton and Adhesive Structures

Phenotypic Plasticity of Cancer Cells Based on Remodeling of the Actin Cytoskeleton and Adhesive Structures

A Portable Controllable Compressive Stress Device to Monitor Human Breast Cancer Cell Protrusions at Single-Cell Resolution

Regulation of the Actin Cytoskeleton via Rho GTPase Signalling in Dictyostelium and Mammalian Cells: A Parallel Slalom

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