Actin Cytoskeleton: Profilin Gives Cells an Edge

Rotty, Jeremy D.

doi:10.1016/j.cub.2020.05.041

Cited by 7 publications

(3 citation statements)

References 22 publications

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“…Out of all the HKGs tested, the ribosomal gene Rplp2, which is involved in cell metabolism and regulation [73], showed the best stability ranking in all tissues pre-and post-injury, except for the kidney, and seems to be a safe choice for accurate expression rate determinations for tissue target genes with relatively high endogenous expression levels under the experimental conditions assessed in this study. HKGs widely used to standardize both mRNA transcript and protein levels in tissues, such as Gapdh, Actb, and Tbp, which play major regulatory roles in cellular activation, proliferation, and differentiation activities [43][44][45]48,49,55,74], were ranked as the least stable in both states. The mRNA translation levels were similarly highly variable across the four studied healthy tissues.…”

Section: Discussionmentioning

confidence: 99%

Systematic Identification of the Optimal Housekeeping Genes for Accurate Transcriptomic and Proteomic Profiling of Tissues following Complex Traumatic Injury

Dragon

Rowe

Rhodes

et al. 2023

MPs

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Trauma triggers critical molecular and cellular signaling cascades that drive biological outcomes and recovery. Variations in the gene expression of common endogenous reference housekeeping genes (HKGs) used in data normalization differ between tissue types and pathological states. Systematically, we investigated the gene stability of nine HKGs (Actb, B2m, Gapdh, Hprt1, Pgk1, Rplp0, Rplp2, Tbp, and Tfrc) from tissues prone to remote organ dysfunction (lung, liver, kidney, and muscle) following extremity trauma. Computational algorithms (geNorm, Normfinder, ΔCt, BestKeeper, RefFinder) were applied to estimate the expression stability of each HKG or combinations of them, within and between tissues, under both steady-state and systemic inflammatory conditions. Rplp2 was ranked as the most suitable in the healthy and injured lung, kidney, and skeletal muscle, whereas Rplp2 and either Hprt1 or Pgk1 were the most suitable in the healthy and injured liver, respectively. However, the geometric mean of the three most stable genes was deemed the most stable internal reference control. Actb and Tbp were the least stable in normal tissues, whereas Gapdh and Tbp were the least stable across all tissues post-trauma. Ct values correlated poorly with the translation from mRNA to protein. Our results provide a valuable resource for the accurate normalization of gene expression in trauma-related experiments.

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Section: Discussionmentioning

confidence: 99%

Systematic Identification of the Optimal Housekeeping Genes for Accurate Transcriptomic and Proteomic Profiling of Tissues following Complex Traumatic Injury

Dragon

Rowe

Rhodes

et al. 2023

MPs

View full text Add to dashboard Cite

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“…Numerous studies underscore the unbalanced expression of actin-binding and regulatory proteins in cancer. For example, decreased expression of Profilin 1 protein as an actin-binding protein has been observed in breast cancer 29 . In addition, decreased expression of Arp2/3 and N-Wasp have been reported in gastric cancer and breast cancer, respectively 30 , 31 .…”

Section: Discussionmentioning

confidence: 99%

The risk of pancreatic adenocarcinoma following SARS-CoV family infection

Sadrabadi

Bereimipour

Jalili

et al. 2021

Sci Rep

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COVID 19 disease has become a global catastrophe over the past year that has claimed the lives of over two million people around the world. Despite the introduction of vaccines against the disease, there is still a long way to completely eradicate it. There are concerns about the complications following infection with SARS-CoV-2. This research aimed to evaluate the possible correlation between infection with SARS-CoV viruses and cancer in an in-silico study model. To do this, the relevent dataset was selected from GEO database. Identification of differentially expressed genes among defined groups including SARS-CoV, SARS-dORF6, SARS-BatSRBD, and H1N1 were screened where the |Log FC| ≥ 1and p < 0.05 were considered statistically significant. Later, the pathway enrichment analysis and gene ontology (GO) were used by Enrichr and Shiny GO databases. Evaluation with STRING online was applied to predict the functional interactions of proteins, followed by Cytoscape analysis to identify the master genes. Finally, analysis with GEPIA2 server was carried out to reveal the possible correlation between candidate genes and cancer development. The results showed that the main molecular function of up- and down-regulated genes was “double-stranded RNA binding” and actin-binding, respectively. STRING and Cytoscape analysis presented four genes, PTEN, CREB1, CASP3, and SMAD3 as the key genes involved in cancer development. According to TCGA database results, these four genes were up-regulated notably in pancreatic adenocarcinoma. Our findings suggest that pancreatic adenocarcinoma is the most probably malignancy happening after infection with SARS-CoV family.

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“…On the other hand, in cancer cells with high CFL1/actin ratio, CFL1 binds rapidly to F-actin, causing its saturation and stabilization into twisted form that allow its separation as CFL1-saturated actin bundles from the pointed ends. CFL1 rapidly dissociates from actin, causing the emergence of new highly-growing barbed ends which in turn induces cellular motility [ 80 – 83 ]. The phosphorylation/ dephosphorylation status of CFL1 ascertain its activity, as CFL1 phosphorylation inhibits its activity and ability to bind F-actin and hence actin filaments remodeling [ 84 ].…”

Section: Introductionmentioning

confidence: 99%

Involvement of lncRNAs in cancer cells migration, invasion and metastasis: cytoskeleton and ECM crosstalk

Ahmad

Weiswald

Poulain

et al. 2023

J Exp Clin Cancer Res

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Cancer is the main cause of death worldwide and metastasis is a major cause of poor prognosis and cancer-associated mortality. Metastatic conversion of cancer cells is a multiplex process, including EMT through cytoskeleton remodeling and interaction with TME. Tens of thousands of putative lncRNAs have been identified, but the biological functions of most are still to be identified. However, lncRNAs have already emerged as key regulators of gene expression at transcriptional and post-transcriptional level to control gene expression in a spatio-temporal fashion. LncRNA-dependent mechanisms can control cell fates during development and their perturbed expression is associated with the onset and progression of many diseases including cancer. LncRNAs have been involved in each step of cancer cells metastasis through different modes of action. The investigation of lncRNAs different roles in cancer metastasis could possibly lead to the identification of new biomarkers and innovative cancer therapeutic options.

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Actin Cytoskeleton: Profilin Gives Cells an Edge

Cited by 7 publications

References 22 publications

Systematic Identification of the Optimal Housekeeping Genes for Accurate Transcriptomic and Proteomic Profiling of Tissues following Complex Traumatic Injury

Systematic Identification of the Optimal Housekeeping Genes for Accurate Transcriptomic and Proteomic Profiling of Tissues following Complex Traumatic Injury

The risk of pancreatic adenocarcinoma following SARS-CoV family infection

Involvement of lncRNAs in cancer cells migration, invasion and metastasis: cytoskeleton and ECM crosstalk

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