Actin Gene-Based Molecular Typing of Trichomonas vaginalis Clinical Isolates from the North of Iran

Hezarjaribi, Hajar Ziaei; Taghavi, Mahbobeh; Saravi, Karan Hasanjani; Faridnia, Roghiyeh; Kalani, Hamed; Mardani, Ahmad; Jorjani, Ogholniaz; Hosseini‐Khah, Zahra; Esboei, Bahman Rahimi; Gholami, Moeen; Fakhar, Mahdi

doi:10.2478/s11686-020-00226-x

Cited by 5 publications

(8 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the actin gene can be used as a unique indicator owing to its structural role in organisms (10). Following the amplification of the actin gene, we found the most common genotypes to be G and E. This is consistent with the results of Hezarjaribi et al (23) who also used the actin gene to show that the genotypes G, E, and I were the predominant genotypes in women and men in northern Iran. Lastly, using PCR-RFLP patterns, sequencing, and phylogenetic analyses, Spotin et al (37) found the most predominant genotypes in asymptomatic patients of Tabriz in northwest Iran to be G (73.4%) and E (26.6%).…”

Section: Discussionsupporting

confidence: 91%

“…A study that analyzed 1353 pap smears in women from Ahvaz city in southwest Iran, also reported similar findings where 1.4% of patients were infected with T. vaginalis (22). In Mazandaran, a province in northern Iran, PCR results showed that 0.48% of pregnant women referred to health centers were infected with T. vaginalis (23). The frequency distribution of T. vaginalis infection in Iran also varies (0.5% to about 39%) amongst different age groups of women and geographical location (24).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Trichomonas Vaginalis Genotypes Using by Actin Gene and Molecular Based Methods in Southwest of Iran

et al. 2021

View full text Add to dashboard Cite

Background:Trichomonas vaginalis (T. vaginalis) is a sexually transmitted protozoan parasite and the causative agent of trichomoniasis. The genetic characterization of T. vaginalis isolates shows notable genetic variation in this parasite. In the present study, we aimed to identify the T. vaginalis genotypes based on analyzing of actin gene in women specimens referred to health centers of Ilam city, southwest Iran. Methods: A total of 1765 female samples were collected from gynecology clinics in the city of Ilam. DNA was extracted from positive samples and nested polymerase chain reaction (Nested PCR) was used to amplify the actin gene. Then, partial sequencing and genotyping of the actin gene was performed. A phylogenetic tree was drawn using the detected genotypes of T. vaginalis and reference sequences. Results: Twenty-one of the 1765 urine and vaginal samples were positive for T. vaginalis. All infected individuals were married and their age in years was between 25 to 34. Further, the majority of infected women had cervical lesions, patchy erythema, and white color discharge. According to sequencing analysis, the isolates were identified as genotype G (n= 8) and genotype E (n= 2). Conclusions: From the collected samples, we were able to distinguish at least two genotypes (G and E) of T. vaginalis. However, lesser is known about these genotypes in the city of Ilam. Further studies with a higher number of isolates should be performed in order to understand the implications of these results in this region.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Identification of Trichomonas Vaginalis Genotypes Using by Actin Gene and Molecular Based Methods in Southwest of Iran

et al. 2021

View full text Add to dashboard Cite

show abstract

“…It has been observed that T. vaginalis carrying virus (TVV) increases T. vaginalis toxicity, upregulates proinflammatory responses in the host and increases T. vaginalis adherence to host cells [ 1 ]. Simultaneously, it has been shown that the etiology of T. vaginalis resistance to metronidazole, the preferred treatment for trichomoniasis, is associated with multiple single nucleotide alterations in the actin gene [ 24 , 30 ]. Since the genotype of T. vaginalis and whether this parasite carries TVVs play important roles in the pathogenesis and drug resistance of T. vaginalis [ 24 ], identifying the genotype of the parasite and the existence of TVVs can help provide a theoretical foundation for parasite control.…”

Section: Discussionmentioning

confidence: 99%

“…Simultaneously, it has been shown that the etiology of T. vaginalis resistance to metronidazole, the preferred treatment for trichomoniasis, is associated with multiple single nucleotide alterations in the actin gene [ 24 , 30 ]. Since the genotype of T. vaginalis and whether this parasite carries TVVs play important roles in the pathogenesis and drug resistance of T. vaginalis [ 24 ], identifying the genotype of the parasite and the existence of TVVs can help provide a theoretical foundation for parasite control. We used the target gene to compare with the t seven genotypes of T. vaginalis (E, G, H, I, M, N and P) (Additional file 1 : Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The actin gene products of positive samples and the T. vaginalis standard strain were collected and digested by HindII, MseI and RsaI at 37 °C for 4 h, and eight different actin types (A E G H I M N P) were identified according to the location and number of cleavage sites (Table 2 ) [ 24 ]. The digested fragments were separated on a 3% agarose gel by electrophoresis and observed under UV light, and fragment size was assessed.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Establishment and application of a CRISPR-Cas12a-based RPA-LFS and fluorescence for the detection of Trichomonas vaginalis

Wang

et al. 2022

Parasites Vectors

View full text Add to dashboard Cite

Background Infection with Trichomonas vaginalis can lead to cervicitis, urethritis, pelvic inflammatory disease, prostatitis and perinatal complications and increased risk of HIV transmission. Here, we used an RPA-based CRISPR-Cas12a assay system in combination with a lateral flow strip (LFS) (referred to as RPA-CRISPR-Cas12a) to establish a highly sensitive and field-ready assay and evaluated its ability to detect clinical samples. Methods We developed a one-pot CRISPR-Cas12a combined with RPA-based field detection technology for T. vaginalis, chose actin as the target gene to design crRNA and designed RPA primers based on the crRNA binding site. The specificity of the method was demonstrated by detecting genomes from nine pathogens. To improve the usability and visualize the RPA-CRISPR-Cas12a assay results, both fluorescence detection and LFS readouts were devised. Results The RPA-CRISPR-Cas12a assay platform was completed within 60 min and had a maximum detection limit of 1 copy/µl and no cross-reactivity with Candida albicans, Mycoplasma hominis, Neisseria gonorrhoeae, Escherichia coli, Cryptosporidium parvum, G. duodenalis or Toxoplasma gondii after specificity validation. Thirty human vaginal secretions were tested by RPA-CRISPR-Cas12a assays, and the results were read by a fluorescent reporter and LFS biosensors and then compared to the results from nested PCR detection of these samples. Both RPA-CRISPR-Cas12a assays showed 26.7% (8/30) T. vaginalis-positive samples and a consistency of 100% (8/8). The RPA-CRISPR-Cas12a assays had a higher sensitivity than nested PCR (only seven T. vaginalis-positive samples were detected). Conclusions The T. vaginalis RPA-CRISPR-Cas12a assay platform in this study can be used for large-scale field testing and on-site tests without the need for trained technicians or costly ancillary equipment. Graphical abstract

show abstract

Characterization of Actin-based Genotypes and Mycoplasma Endosymbionts of Trichomonas vaginalis Isolated in Sapporo, Japan

Yamazaki,

Sawai,

Takahashi

et al. 2024

Acta Parasit.

View full text Add to dashboard Cite

Actin Gene-Based Molecular Typing of Trichomonas vaginalis Clinical Isolates from the North of Iran

Cited by 5 publications

References 22 publications

Identification of Trichomonas Vaginalis Genotypes Using by Actin Gene and Molecular Based Methods in Southwest of Iran

Identification of Trichomonas Vaginalis Genotypes Using by Actin Gene and Molecular Based Methods in Southwest of Iran

Establishment and application of a CRISPR-Cas12a-based RPA-LFS and fluorescence for the detection of Trichomonas vaginalis

Characterization of Actin-based Genotypes and Mycoplasma Endosymbionts of Trichomonas vaginalis Isolated in Sapporo, Japan

Contact Info

Product

Resources

About