Actin gene expression is modulated by ecdysterone in a Drosophila cell line

Couderc, J.L.; Sobrier, Marie-Laure; Giraud, G; Becker, Jacqueline; Dastugue, B.

doi:10.1016/0022-2836(83)90059-1

Cited by 27 publications

(10 citation statements)

References 24 publications

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“…Indeed, it was also observed that the number of the "copia-like" element B104 per haploid genome is higher is cultured cells compared to flies (35). Contrary to "copia like" elements, we have not observed qualitative or quantitative rearrangements in the six actin genes pattern between flies and Kc cells (34).…”

Section: Discussioncontrasting

confidence: 51%

Molecular study of the retrovirus-like transposable element 412, a 20-OH ecdysone responsive repetitive sequence inDrosophilacultured cells

et al. 1988

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Used at a physiological concentration, the steroid hormone 20-hydroxyecdysone (20-OHE) induces, in Kc cultured Drosophila melanogaster cells, important and specific changes. Modifications occur at morphological and enzymatical levels. Variations in specific protein synthesis are observed. At the molecular level, 20-OHE particularly induces a decrease in expression of the mobile dispersed genetic element 412. This repeated element which belongs to the "copia-like" family is more widely represented in Kc cells (80 fold) compared to fly cells (25 fold). 412 transcripts are heterogeneous in size, essentially polyadenylated and restricted to the nucleus. A minimal concentration of 10(-8) M and a time treatment of 16 hours are necessary to obtain a strong decrease in 412 expression. The decrease is at least an effect on these sequences at the transcriptional level. Structural similarities between the 412 element and the proviral forms of vertebrate retroviruses are strengthened by the characterization of extrachromosomal circular DNA forms revealed by the 412 probe. Quantifying experiments have shown that the steady state level of such forms is not affected by the steroid treatment.

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Section: Discussioncontrasting

confidence: 51%

Molecular study of the retrovirus-like transposable element 412, a 20-OH ecdysone responsive repetitive sequence inDrosophilacultured cells

et al. 1988

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“…The titer ofecdysone decreases in the prepupal period when the OSA antigens become undetectable in the disk epithelium, increases very sharply at stage 3 when the antigens reappear in the ORCs in the antenna1 epithelium, and rapidly declines at stage 10 when the antigen species change. Ecdysone, which is one of the major steroid hormones in invertebrates (Riddiford and Truman, 1978;Granger and Bollenbacher, 198 l), transcriptionally regulates the expression of several genes in a Drosophifu cell line (Cherbas et al, 1977;Best-Belpomme et al, 1978;Savakis et al, 1980;Couderc et al, 1983;Savakis et al, 1984). If ecdysone is similarly involved in the regulation of the OSA antigens, one might hypothesize that the differential expression of each antigen recognized by the OSA during postembryonic development is regulated by ecdysone either via the expression of a family ofgenes, each of which encodes for one of the OSA antigens, or via induction of expression of a gene coding for an enzyme that modifies transcriptional or translational products.…”

Section: Discussionmentioning

confidence: 99%

Monoclonal antibodies reveal cell-type-specific antigens in the sexually dimorphic olfactory system of Manduca sexta. II. Expression of antigens during postembryonic development

et al. 1988

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Two classes of monoclonal antibodies specific to the olfactory system of Manduca sexta have been isolated: the olfactory-specific antibody (OSA), which specifically recognizes many or all olfactory receptor cells (ORCs) in both males and females, and the male olfactory-specific antibody (MOSA), which stains male-specific receptor cells (principally or exclusively sex-pheromone receptors present only in antennae of males; Hishinuma et al., 1988). In the investigation reported here, we examined the expression of the antigens during postembryonic development in order to correlate the presence of particular antigens with the status of differentiation of the ORCs or with their acquisition of particular functions. As assessed immunocytochemically, the OSA recognizes certain epithelial cells in the antennal imaginal disk of the fifth-instar larva. Later, during the first 70 hr of adult development, when differentiative cell divisions are occurring in the antennal epithelium to generate ORCs and the other cells that make up olfactory sensilla, no cells are stained. Immediately after this period of mitoses, the OSA immunoreactivity reappears exclusively in the ORCs, which begin to elaborate axons as an early event in their differentiation. On immunoblots, the OSA recognizes specific sets of molecules (distinguished on the basis of their apparent molecular weights): 53,000 and 59,000 Da antigens in the disk epithelial cells in the last-instar larva; 53,000, 59,000, and 66,000 Da antigens in the ORCs from 15 to 60% of metamorphic adult development; and 42,000, 59,000, and 66,000 Da antigens in the ORCs from 60 to 100% of adult development. The MOSA also recognizes a subset of the epithelial cells in the antennal disks in male and female larvae.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…The 5C gene encodes a cytoplasmic actin (Fyrberg et al 1980) and it also has been shown to respond to hormonal stimulus (Couderc et al 1983). Therefore, the existence of cis-regulatory elements that mediate 5C actin response to an array of transcription-controlling factors is not unlikely.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, Couderc et al (1983) have shown that expression of the 5C actin gene is modulated by the molting hormone ecdysterone. The 5C actin gene differs from the other five Drosophila actin genes in one more respect: It is the only actin gene that is transcribed into developmentally regulated transcripts of different size classes (Fyrberg et al 1983;Vigoreaux 1987).…”

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confidence: 99%

Stage-specific selection of alternative transcriptional initiation sites from the 5C actin gene of Drosophila melanogaster.

Vigoreaux¹,

Tobin²

1987

Genes Dev.

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The transcription unit of the 5C actin gene exhibits a complex organization that is unique among the six actin genes of Drosophila melanogaster. Three different mRNA size classes showing distinct patterns of accumulation throughout development are detected on Northern blots. We have determined the structure of the various 5C actin transcripts by exon mapping using strand-specific RNA probes, primer extension analysis, and DNA sequences analysis of both cDNA and genomic clones. All the transcripts share a single protein-coding nucleotide sequence but are heterogeneous in the 5' and 3' untranslated regions. The 5' untranslated region of each transcript consists of either one of two small exons (exon 1 and exon 2) which are alternatively spliced to a single acceptor site 8 bp upstream from the translation initiation codon in exon 3. Results from primer extension analysis suggest that transcription can initiate from either exon 1 or exon 2, and also from a third site within exon 2. We detect an increase in the relative abundance of exon 1-containing transcripts at larval and pupal stages, as well as a change in the proportion of transcripts that initiate at either of the two exon 2 sites. Five polyadenylation sites have been found within three termination/processing regions that define the three size classes of polyadenylated transcripts. The results of our experiments indicate the existence in vivo of all possible combinations of 5' exon with 3' polyadenylation site. However, particular combinations of 5' initiation site and 3' polyadenylation site are preferred at certain developmental stages.

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Actin gene expression is modulated by ecdysterone in a Drosophila cell line

Cited by 27 publications

References 24 publications

Molecular study of the retrovirus-like transposable element 412, a 20-OH ecdysone responsive repetitive sequence inDrosophilacultured cells

Molecular study of the retrovirus-like transposable element 412, a 20-OH ecdysone responsive repetitive sequence inDrosophilacultured cells

Monoclonal antibodies reveal cell-type-specific antigens in the sexually dimorphic olfactory system of Manduca sexta. II. Expression of antigens during postembryonic development

Stage-specific selection of alternative transcriptional initiation sites from the 5C actin gene of Drosophila melanogaster.

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