Actin-Interacting Proteins in flagellated pathogenic Leishmania spp.: a genome-based bioinformatics report on profilins, formins and katanins

Vasconcelos, Elton J R; Pacheco, Ana Carolina Landim; Gouveia, João José de Simoni; Araújo, Fabiana F.; Diniz, Michely Correia; Kamimura, Michel T.; Costa, Marcília Pinheiro da; Araújo-Filho, Raimundo; Oliveira, Diana Magalhães de

doi:10.1504/ijfipm.2008.021389

Cited by 3 publications

(10 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our underlying hypothesis is that polymerization and depolymerization of parasite actin and actin motor-associated proteins, during both processes of motility and host cell entry, might be key events for successful infection, including the parasite survival within phagosomes. Recently ( Vasconcelos et al , 2007 , 2008 ) we reported an actin-polymerization Leishmania protein, profilin, and its partner, formin, as putative flagellar proteins due to their likely involvement in axonemal assembly/disassembly, therefore, flagellar dynamics/remodeling. In addition, we predicted other Leishmania AIPs, coronins and Arp2/3 complex as being flagellar proteins after detailed in silico structural alignments ( Costa et al , 2007 ).…”

Section: Resultsmentioning

confidence: 99%

“…As previously ( Oliveira et al , 2005 ; Gouveia et al , 2007 ; Vasconcelos et al , 2007 , 2008 ), we have used publicly available datasets of individual/clusters of gene/protein data on Leishmania spp . (from GeneDB, a core part of Sanger Institute Pathogen Genomics) and from other available eukaryotic organisms (at NCBI and UniProt/Swiss-Prot/trEMBL knowledge DB), including sequences from the genome projects of C. reinhardtii in the U.S. Department of Energy (JGI) and all available data at The Chlamydomonas Flagellar Proteome Project.…”

Section: Methodsmentioning

confidence: 99%

“…For databases (DB) searches, accession numbers/identifiers are those used in four main sources: NCBI, GeneDB, PDB and UniProt. As previously described ( Oliveira et al , 2005 ; Gouveia et al , 2007 ; Vasconcelos et al , 2007 , 2008 ), BLAST and its variants ( Altschul et al , 1997 ) and MUSCLE ( Edgar, 2004 ) were used for sequence similarity searches and comparisons/analysis through pairwise and multiple alignments. As previously detailed ( Costa et al , 2007 ), for pattern recognition tasks of gene/protein predictions, motif finding and refinements for defining core domains of interest, HMM implementations such as HMMER ( Eddy, 1998 ) and a structural prediction adaptation ( Yoshizawa et al , 2006 ) were employed, with the additional improvement of the Viterbi algorithm (VA) ( Forney, 1973 ; Black, 2004 ) for best possible alignments after recursion ( Alexandrov and Gerstein, 2004 ).…”

Section: Methodsmentioning

confidence: 99%

“…As described in previous studies ( Oliveira et al , 2005 ; Gouveia et al , 2007 ; Vasconcelos et al 2008 ), we took alignments created with FASTA/BLAST as input and computed alignment tables, providing hierarchical and successive correlations between each of the two sets of sequences. FASTA files for amino-acid (aa) sequences of coding regions were downloaded from sources detailed above.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Actin-interacting and flagellar proteins in Leishmania spp.: bioinformatics predictions to functional assignments in phagosome formation

et al. 2009

Self Cite

View full text Add to dashboard Cite

Several motile processes are responsible for the movement of proteins into and within the flagellar membrane, but little is known about the process by which specific proteins (either actin-associated or not) are targeted to protozoan flagellar membranes. Actin is a major cytoskeleton protein, while polymerization and depolymerization of parasite actin and actin-interacting proteins (AIPs) during both processes of motility and host cell entry might be key events for successful infection. For a better understanding the eukaryotic flagellar dynamics, we have surveyed genomes, transcriptomes and proteomes of pathogenic Leishmania spp. to identify pertinent genes/proteins and to build in silico models to properly address their putative roles in trypanosomatid virulence. In a search for AIPs involved in flagellar activities, we applied computational biology and proteomic tools to infer from the biological meaning of coronins and Arp2/3, two important elements in phagosome formation after parasite phagocytosis by macrophages. Results presented here provide the first report of Leishmania coronin and Arp2/3 as flagellar proteins that also might be involved in phagosome formation through actin polymerization within the flagellar environment. This is an issue worthy of further in vitro examination that remains now as a direct, positive bioinformatics-derived inference to be presented.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Actin-interacting and flagellar proteins in Leishmania spp.: bioinformatics predictions to functional assignments in phagosome formation

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…There are reports of homologs to members of the IFT complex proteins in several organisms, including trypanosomatids such as Trypanosoma [40, 97-100] and Leishmania , the latter that has been our own focus upon the eukaryotic flagellum [101-103]. The first work to provide the actual demonstration of IFT in Trypanosoma brucei [100] also revealed the activity of this process in both old (in maintenance) and new flagellum (in construction) in the same cell.…”

Section: Conserved To Diverge In Flagellar Assembly and Disassemblymentioning

confidence: 99%

The Eukaryotic Flagellum Makes the Day: Novel and Unforeseen Roles Uncovered After Post-Genomics and Proteomics Data

Diniz

Pacheco

Farias³

et al. 2012

CPPS

View full text Add to dashboard Cite

This review will summarize and discuss the current biological understanding of the motile eukaryotic flagellum, as posed out by recent advances enabled by post-genomics and proteomics approaches. The organelle, which is crucial for motility, survival, differentiation, reproduction, division and feeding, among other activities, of many eukaryotes, is a great example of a natural nanomachine assembled mostly by proteins (around 350-650 of them) that have been conserved throughout eukaryotic evolution. Flagellar proteins are discussed in terms of their arrangement on to the axoneme, the canonical “9+2” microtubule pattern, and also motor and sensorial elements that have been detected by recent proteomic analyses in organisms such as Chlamydomonas reinhardtii, sea urchin, and trypanosomatids. Such findings can be remarkably matched up to important discoveries in vertebrate and mammalian types as diverse as sperm cells, ciliated kidney epithelia, respiratory and oviductal cilia, and neuro-epithelia, among others. Here we will focus on some exciting work regarding eukaryotic flagellar proteins, particularly using the flagellar proteome of C. reinhardtii as a reference map for exploring motility in function, dysfunction and pathogenic flagellates. The reference map for the eukaryotic flagellar proteome consists of 652 proteins that include known structural and intraflagellar transport (IFT) proteins, less well-characterized signal transduction proteins and flagellar associated proteins (FAPs), besides almost two hundred unannotated conserved proteins, which lately have been the subject of intense investigation and of our present examination.

show abstract

Formins play important role inLeishmaniaphysiology by acting as cytosolic actin bundlers

Kushwaha

Seth

Jijumon

et al. 2021

Preprint

View full text Add to dashboard Cite

Formin proteins regulate actin dynamics, are conserved throughout the eukaryotes cells. They play an important role in cell adhesion, motility, vesicular trafficking, and cytokinesis. Formins from class Kinetoplastida which includes infective organisms such as Leishmania and Trypanosoma not characterized to date, even though they are shown to be important in other protozoan parasites. The protozoan parasite Leishmania major (Lm) has two homologous formin proteins; LmForminA and LmForminB. Our study showed that LmForminA and LmForminB are expressed at RNA and protein levels in L. major cells. LmForminA and LmForminB are localized in the cytosol in patchy distribution patterns. LmForminA and LmForminB puncta also colocalize with the actin patches. The biochemical properties of L. major formins divulge that both formins are potent actin nucleator. LmForminA and LmForminB bind with the actin filament and have actin-bundling activity. We have also observed that formin inhibitor SMIFH2 influences the growth and physiology of L. major cells indicating formins are important for the Leishmania parasite.

show abstract

Actin-Interacting Proteins in flagellated pathogenic Leishmania spp.: a genome-based bioinformatics report on profilins, formins and katanins

Cited by 3 publications

References 53 publications

Actin-interacting and flagellar proteins in Leishmania spp.: bioinformatics predictions to functional assignments in phagosome formation

Actin-interacting and flagellar proteins in Leishmania spp.: bioinformatics predictions to functional assignments in phagosome formation

The Eukaryotic Flagellum Makes the Day: Novel and Unforeseen Roles Uncovered After Post-Genomics and Proteomics Data

Formins play important role inLeishmaniaphysiology by acting as cytosolic actin bundlers

Contact Info

Product

Resources

About