Actinobacillus pleuropneumoniae infections in closed swine herds: infection patterns and serological profiles

Chiers, Koen; Donné, Eef; Overbeke, I. Van; Ducatelle, Richard; Haesebrouck, Freddy

doi:10.1016/s0378-1135(01)00518-1

Cited by 81 publications

(70 citation statements)

References 15 publications

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“…This is suggestive of loss of protection due to waning of passively acquired antibodies, which can occur anytime between 5 to 12 weeks. 6,8,46 The different A. pleuropneumoniae serovars vary in their ability to induce clinical disease; however, with some of the more virulent serovars, clinical signs may develop as quick as 4-12 hr postinfection. 30 Actinobacillus pleuropneumoniae immunoglobulin M (IgM) antibodies have been detected as early as 1 week after experimental infection.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of diagnostic assays for the serological detection ofActinobacillus pleuropneumoniaeon samples of known or unknown exposure

et al. 2013

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Abstract. Accurate diagnosis of exposure to Actinobacillus pleuropneumoniae is important for maintaining negative farms. In the present study, the ability of a dual-plate complement fixation (CF) assay and 3 commercially available enzymelinked immunosorbent assays (ELISAs; quad-plate ELISA-1, single-plate ELISA-2, and single-plate ELISA-3) in detecting serological evidence of A. pleuropneumoniae exposure was compared using serum samples of experimentally infected or vaccinated pigs, or field samples from the United States. Forty-two pigs were divided into groups of 2 pigs and were inoculated with 1 of 15 A. pleuropneumoniae strains representing all known serovars of A. pleuropneumoniae, or with Actinobacillus suis, or were vaccinated with a bacterin containing A. pleuropneumoniae serovar 1, 3, 5, or 7. Serum samples collected at the day of inoculation or vaccination and 7, 14, 21, and 28 days later were used to compare the assays. On samples from experimentally infected pigs, the dual-plate CF assay, quad-plate ELISA-1, single-plate ELISA-2, and single-plate ELISA-3 had sensitivities of 0.46, 0.74, 0.13, and 0.13 and specificities of 0.90, 1.0, 1.0, and 1.0, respectively. Vaccinated pigs were identified only by the dual-plate CF assay and the quad-plate ELISA-1. In addition, 90 serum samples with unknown A. pleuropneumoniae exposure collected under field conditions were tested with all assays. The agreement of the 4 assays on field samples was slight to fair. While several assays are available for demonstration of A. pleuropneumoniae exposure, differences in assay targets complicate test choices. Decisions on which assay or combination of assays to use depend on the specific reasons for running the assays.

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Section: Introductionmentioning

confidence: 99%

Evaluation of diagnostic assays for the serological detection ofActinobacillus pleuropneumoniaeon samples of known or unknown exposure

et al. 2013

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“…En todo caso, se podría estar frente a una fuerte transferencia de la inmunidad pasiva (García, 2007). No obstante, Haesebrouck et al (1997) señalan que la protección de los anticuerpos maternales no supera las 3 semanas; además, se reconoce que la prueba de ELISA indirecta detecta generalmente anticuerpos a partir de las 3 semanas post infección (Dreyfus et al, 2004) los animales se tornan susceptibles a la infección a partir de las 8 semanas de vida (Chiers et al, 2002), de allí que se podría sugerir que la seropositividad encontrada a partir de los 60 días de edad puede ser consecuencia de una probable infección activa.…”

Section: Resultados Y Discusiónunclassified

Frecuencia De Infección Con Actinobacillus Pleuropneumoniae en Granjas Porcinas Tecnificadas De La Costa Peruana

Mori

Calle

Pinto

et al. 2011

Rev. investig. vet. Perú

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“…Researchers have indicated that the natural habitat of the agent in pigs is in the respiratory passages, and for identification purposes, lung, tonsil and nasal swab samples are suitable (Chiers et al 2002;L o 1998;Gram et al 1996;Sidibe et al 1993;Mittal et al1984). By comparing nasal swab and tonsil samples in terms of recovery of the agent, researchers have claimed that isolation rates from tonsil samples are more appropriate than those from nasal swab samples.…”

Section: Discussionmentioning

confidence: 99%

“…By comparing nasal swab and tonsil samples in terms of recovery of the agent, researchers have claimed that isolation rates from tonsil samples are more appropriate than those from nasal swab samples. Therefore, tonsil samples should be used for isolation purposes (Chiers et al 2002;Sidibe et al 1993). Wilson et al (1987) have indicated that bacteriological culture is specific.…”

Section: Discussionmentioning

confidence: 99%

Presence and Seroprevalence of Actinobacillus pleuropneumoniae in Pigs in Turkey

Metiner

2007

Acta Vet. Brno

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Metiner K., S. Ak: Presence and Seroprevalence of Actinobacillus pleuropneumoniae in Pigs in Turkey. Acta Vet Brno 2007, 76: 237-244. In this study, the presence and seroprevalence of Actinobacillus pleuropneumoniae in pigs raised in various regions of Turkey were investigated. For this purpose, 384 lung, tonsil and blood samples were taken by random sampling during the slaughter. The pigs did not show any signs of clinical respiratory tract disorders at the time of slaughter. Organ samples were used for isolation, and specific antibodies in 384 sera samples (of which 368 belonged to animals used in organ sampling) were investigated by enzyme-linked immunosorbent assay (ELISA). For isolation, the lung and tonsil samples were inoculated onto PPLO agar plates with nicotinamide adenine dinucleotide (NAD), and A. pleuropneumoniae (serotype 12) was isolated from only one of the tonsil samples. Of the 384 blood samples, 258 (67.2%) were found to be positive. 209 (54.4%) of the positive samples were determined to be group 1, and 49 (12.8%) were determined to be group 2.The effect of age and sex of the samples were found to be non-significant by statistical analysis of the ELISA data (p > 0.05).Isolation of A. pleuropneumoniae indicates the presence of the infection in Turkey for the first time and high seroprevalence supports this finding.

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Actinobacillus pleuropneumoniae infections in closed swine herds: infection patterns and serological profiles

Cited by 81 publications

References 15 publications

Evaluation of diagnostic assays for the serological detection ofActinobacillus pleuropneumoniaeon samples of known or unknown exposure

Evaluation of diagnostic assays for the serological detection ofActinobacillus pleuropneumoniaeon samples of known or unknown exposure

Frecuencia De Infección Con Actinobacillus Pleuropneumoniae en Granjas Porcinas Tecnificadas De La Costa Peruana

Presence and Seroprevalence of Actinobacillus pleuropneumoniae in Pigs in Turkey

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