Actinobacteria possessing antimicrobial and antioxidant activities isolated from the pollen of scots pine (Pinus sylvestris) grown on the Baikal shore

Abstract

Isolated ecosystems existing under specific environmental conditions have been shown to be promising sources of new strains of actinobacteria. The taiga forest of Baikal Siberia has not been well studied, and its actinobacterial population remains uncharacterized. The proximity between the huge water mass of Lake Baikal and high mountain ranges influences the structure and diversity of the plant world in Siberia. Here, we report the isolation of eighteen actinobacterial strains from male cones of Scots pine trees (Pinus sylvestris) growing on the shore of the ancient Lake Baikal in Siberia. In addition to more common representative strains of Streptomyces, several species belonging to the genera Rhodococcus, Amycolatopsis, and Micromonospora were isolated. All isolated strains exhibited antibacterial and antifungal activities. We identified several strains that inhibited the growth of the pathogen Candida albicans but did not hinder the growth of Saccharomyces cerevisiae. Several isolates were active against Gram-positive and Gram-negative bacteria. The high proportion of biologically active strains producing antibacterial and specific antifungal compounds may reflect their role in protecting pollen against phytopathogens.

Cultivable Actinobacteria First Found in Baikal Endemic Algae Is a New Source of Natural Products with Antibiotic Activity

“…We estimated their similarity with other actinobacteria and performed initial description of crude extracts antibiotic activity of bacteria cultivated in different nutrient media. According to the materials obtained in this study, we could preliminarily conclude that diversity of cultivable actinobacteria and rare genera isolated from D. baicalensis is comparable to that of cultivable actinobacteria that we previously isolated from the pollen of a pine species growing on the shore of the lake [29]. At the same time, according to published data, the diversity of rare genera of cultivable actinobacteria isolated here from the alga exceeds the cultivable actinobacteria diversity described for Lake Baikal macroinvertebrates [32,43], water [44,45], or sediments [46,47] (Figure 5).…”

“…In Baikal region, there were only single studies focused on assessment of cultivated actinobacteria diversity in high-plant communities. Thus, in one of the recent studies conducted by Axenov-Gribanov et al [ 29 ], a variety of actinobacteria in Pinus sylvestris pollen was estimated. The authors showed that the taiga forest of Baikal Siberia has not been well studied, and its actinobacteria population remains undescribed.…”

Canola Root–Associated Microbiomes in the Canadian Prairies

“…Some Amycolatopsis spp. have antimicrobial and antifungal activities (Saito et al, 2009 ; Axenov-Gribanov et al, 2016 ), which may explain the positive correlation of this bacterium with canola yield in our experiment. Another OTU related to a commonly observed endophyte, Pedobacter sp., was detected in the roots of canola and wheat in our experiment.…”

“…Endophytism was reported in several Amycolatopsis spp. (Miao et al, 2011 ; Xing et al, 2013 ; Klykleung et al, 2015 ; Axenov-Gribanov et al, 2016 ), and accordingly, an OTU related to Amycolatopsis sp. was detected only in the roots of canola in our study.…”

Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA, Anti-Biofilm and Antioxidant Activities

“…It is widely known that phenolic compounds exhibit a number of biological activities including antioxidant and antibacterial [64,65]. There is also evidence demonstrating that antioxidative compounds exhibit promising anti-MRSA activity [66][67][68].…”