Actinomadura rudentiformis sp. nov., isolated from soil

Abstract

As part of an antibiotic-screening programme, the actinomycete strain HMC1T was isolated from soil collected from the banks of the Gamka River in the Swartberg Nature Reserve, Western Cape Province, South Africa. The isolate produced branching vegetative mycelia, which entwined to form rope-like structures. It exhibited antibiosis against Enterococcus faecium VanA (a vancomycin-resistant clinical strain) and Mycobacterium aurum A+. Organic solvent extracts of the culture filtrate and mycelial mass of strain HMC1T exhibited moderate antibiosis against Mycobacterium aurum A+, Mycobacterium bovis BCG (Tokyo) and Mycobacterium tuberculosis H37RvT. The chemotaxonomic characteristics of strain HMC1T corresponded with those of members of the genus Actinomadura. Furthermore, phylogenetic analysis based on the 16S rRNA gene showed that the strain was closely related to members of the genus Actinomadura. Phenotypic and 16S rRNA gene sequence analyses indicated that strain HMC1T represents a novel species of the genus Actinomadura, for which the name Actinomadura rudentiformis sp. nov. is proposed. The type strain is HMC1T (=DSM 44962T=NRRL B-24458T).

Paratrechina longicornis ants in a tropical dry forest harbor specific Actinobacteria diversity

“…The role of Actinomadura in nature is poorly known, but its widespread distribution suggests an important role in soil ecology . Strains closely related to A. rudentiformis were isolated in this study, which exhibits antibiosis against Entorococcus faecium and some Mycobacterium pathogenic species . Additionally, A. hisbisca produces polyenoles, called pradamicin, that have antifungal and antiviral activity .…”

Actinomadura bangladeshensis sp. nov. and Actinomadura chokoriensis sp. nov.

“…nov. [type strain 3-46-b(3) T 5JCM 13933 T 5MTCC8057 T ] and Actinomadura chokoriensis sp. nov. [type strain 3-45-a(11) T 5JCM 13932 T 5MTCC8056 T ].The application of chemosystematic, numerical phenetic and molecular systematic methods has clarified the taxonomy of the genus Actinomadura and related taxa (Poscher et al, 1985;Kroppenstedt et al, 1990;Ochi et al, 1991;Trujillo & Goodfellow, 1997Quintana et al, 2003a;Lee & Jeong, 2006;Le Roes & Meyers, 2007 (Henssen, 1957;Zhang et al, 2001). Members of these genera form distinct phyletic lines in trees based on 16S rRNA gene sequences (Zhang et al, 1998(Zhang et al, , 2001) and can be distinguished from one another by using a combination of biochemical, chemical, morphological and physiological features (Zhang et al, 1998(Zhang et al, , 2001Trujillo & Goodfellow, 2003).…”

“…Members of these genera form distinct phyletic lines in trees based on 16S rRNA gene sequences (Zhang et al, 1998(Zhang et al, , 2001) and can be distinguished from one another by using a combination of biochemical, chemical, morphological and physiological features (Zhang et al, 1998(Zhang et al, , 2001Trujillo & Goodfellow, 2003). At the time of writing, the genus Actinomadura encompasses 37 recognized species, which can be delineated based on a combination of genotypic and phenotypic data (Kroppenstedt & Goodfellow, 1991;Zhang et al, 2001;Lu et al, 2003;Lee & Jeong, 2006;Le Roes & Meyers, 2007).As part of a screening programme for antibiotic-producing actinomycetes, strains 3-46-b(3) T and 3-45-a(11) T were isolated from sandy soil collected at Chokoria, Cox's Bazar, Bangladesh. The two strains showed morphological characteristics typical of the genera Actinomadura and Nonomuraea.…”

“…The application of chemosystematic, numerical phenetic and molecular systematic methods has clarified the taxonomy of the genus Actinomadura and related taxa (Poscher et al, 1985;Kroppenstedt et al, 1990;Ochi et al, 1991;Trujillo & Goodfellow, 1997Quintana et al, 2003a;Lee & Jeong, 2006;Le Roes & Meyers, 2007). The genus Actinomadura belongs to the family Thermomonosporaceae Stackebrandt et al 1997emend.…”

Actinomadura miaoliensis sp. nov., a thermotolerant polyester-degrading actinomycete

“…On the basis of phenotypic and genotypic data, it is proposed that strain BC 44T-5 T (5FIRDI 002 T 5BCRC 16873 T 5LMG 24335 T ) should be classified as the type strain of a novel species of the genus Actinomadura, Actinomadura miaoliensis sp. nov.The genus Actinomadura was firstly described in 1970 (Lechevalier & Lechevalier, 1970) and currently comprises more than 30 species with validly published names (Lu et al, 2003;Cook et al, 2005;Lee & Jeong, 2006;Wang et al, 2007;le Roes & Meyers, 2007). Members of the genus are aerobic, Gram-positive, non-acid-alcohol-fast, non-motile organisms.…”