Actinomadura soli sp. nov., isolated from the top soil layer on basaltic material in Turkey

Abstract

An actinobacterium, designated 14C53T, was isolated from a soil sample on basaltic material from Samsun, Turkey. The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20–37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT. The genome tree revealed a close relationship between the strain and Actinomadura pelletieri DSM 43383T. However, the digital DNA–DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6–30.2 % and 84.3–85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura . The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%. The major cellular fatty acids of strain 14C53T were C16 : 0 and iso-C16 : 0. Strain 14C53T contained meso-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan. The predominant menaquinones were MK-9(H8) and MK-9(H6). Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Actinomadura soli sp. nov. is proposed, with 14C53T (=DSM 104447T=KCTC 39878T) as the type strain.

Actinomadura terrae sp. nov. and Actinomadura litoris OS3-89, isolated from rhizosphere soil of cactus

“… Actinomadura species are characterized by the possession of a type II/B cell wall ( meso -diaminopimelic acid and madurose as the diagnostic diamino acid and sugar in the cell wall, respectively), phospholipid type I or IV (absence of nitrogenous phospholipids and the presence of glucosamine-containing phospholipids, respectively), and menaquinones of MK-9(H 4 ), MK-9(H 6 ) and MK-9(H 8 ) [7]. At the time of writing, the genus consists of 74 species with validly described names (www.bacterio.net) including those recognized during the past 2 years, Actinomadura decatromicini [9], Actinomadura harenae [10], Actinomadura lepetitiana [11], Actinomadura litoris [12], Actinomadura macrotermitis [13], Actinomadura physcomitrii [14], Actinomadura rubrisoli [15], Actinomadura rubteroloni [13], Actinomadura soli [16] and Actinomadura violacea [17], and its members are widely distributed in diverse habitats such as soils, lichens, marine sources and insects. During a study of the bacterial diversity of soils collected on Mara Island, Republic of Korea, 90 mycelium-forming strains were isolated from rhizosphere soil of a cactus growing on a cliff.…”