Actinomadura syzygii sp. nov., an endophytic actinomycete isolated from the roots of a jambolan plum tree (Syzygium cumini L. Skeels)

Rachniyom, Hathairat; Matsumoto, Atsuko; Indananda, Chantra; Duangmal, Kannika; Takahashi, Yōko; Thamchaipenet, Arinthip

doi:10.1099/ijs.0.000203

Cited by 18 publications

(9 citation statements)

References 42 publications

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“…Information on xylanase-producing Actinomadura species remains limited, although various species, such as A. keratinilytica Cpt20 (Taibi et al 2012), A. miaoliensis TF1 (Sriyapai et al 2018), A. xylanilytica BK147 T (Zucchi et al 2013), A. rudentiformis HMC1 T (le Roes and Meyers 2007), and A. kijaniata SCC 1256 T (Horan and Brodsky 1982), have been reported to display xylanolytic activity. Some Actinomadura species produced other enzymes, such as cellulase Goodfellow 2012, Sriyapai et al 2018), amylase (Horan and Brodsky 1982;Lee and Jeong 2006;Sazak et al 2012;Trujillo and Goodfellow 2012;Rachniyom et al 2015;Zhao et al 2015;Abagana et al 2016;Songsumanus et al 2016;Rachniyom et al 2018), gelatinase (Horan and Brodsky 1982;Mertz and Yao 1986;Zucchi et al 2013), and keratinase (Puhl et al 2009;Trujillo and Goodfellow 2012;Habbeche et al 2014;Elhoul et al 2016), which demonstrated that Actinomadura species are potential enzymes producers. Most of Actinomadura species were mesophilic bacteria, which can grow well at temperatures between 25°C to 40°C.…”

Section: Phylogenetic Tree Analysesmentioning

confidence: 95%

“…The Actinomadura species used in the phylogenetic tree (Figure 2), were mostly isolated from soil (Tomita et al 1989;Wink et al 2003;Lee and Jeong 2006;Tseng et al 2009;Trujillo and Goodfellow 2012;Zucchi et al 2013;Zhao et al 2015;Malisorn et al 2018); some species were associated with plants (Rachniyom et al 2015;Rachniyom et al 2018); one found in biofilters consisting of tree bark compost (Songsumanus et al 2016); and some were pathogens to animals, including humans (Trujillo and Goodfellow 2012). Information on xylanase-producing Actinomadura species remains limited, although various species, such as A. keratinilytica Cpt20 (Taibi et al 2012), A. miaoliensis TF1 (Sriyapai et al 2018), A. xylanilytica BK147 T (Zucchi et al 2013), A. rudentiformis HMC1 T (le Roes and Meyers 2007), and A. kijaniata SCC 1256 T (Horan and Brodsky 1982), have been reported to display xylanolytic activity.…”

Section: Phylogenetic Tree Analysesmentioning

confidence: 99%

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Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Rachmania¹,

Ningsih²,

Setyaningsih³

et al. 2019

Biodiversitas

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Abstract. Rachmania MK, Ningsih F, Setyaningsih PP, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridjal W. 2020. Xylan-degrading ability of thermophilic Actinobacteria isolated from soil in a geothermal area. Biodiversitas 21: 144-154. This study was conducted to obtain the potential isolates of xylan-degrading thermophilic Actinobacteria. Seventeen isolates were obtained from the soil samples collected from Cisolok geysers in West Java, Indonesia. These isolates were screened for their xylan-degrading ability on minimal medium with the addition of 0.5% xylan as substrate, and were incubated at various temperatures for 7 days. A total of 15, 14, 4, and 3 isolates showed a xylan-degrading ability at 45°C, 50°C, 55°C, and 60°C, respectively. The three isolates (SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4) that showed xylan-degrading ability at 60°C on 0.5% xylan were further tested by using minimal medium with the addition of 0.1% RBB-xylan as a substrate. The results showed that these isolates were also able to utilized RBB-xylan at 45 to 60°C. The xylanolytic activity of the crude enzymes from the three isolates on minimal medium containing 0.5% xylan or 0.1% RBB-xylan as substrates, indicated that these isolates were able to produce extracellular xylanase. This study revealed that the soil of Cisolok geysers served as an ideal source for finding the thermophilic Actinobacteria which produced xylanase at high temperatures.

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Section: Phylogenetic Tree Analysesmentioning

confidence: 95%

Section: Phylogenetic Tree Analysesmentioning

confidence: 99%

Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Rachmania¹,

Ningsih²,

Setyaningsih³

et al. 2019

Biodiversitas

View full text Add to dashboard Cite

show abstract

“…As shown in Figure 3, the three isolates and all closely related species are thermophilic, except for three species: A. barringtoniae, A. syzygii, and A. napierensis. These three species have growth temperature lower than 40 o C (Cook et al 2005;Rachniyom et al 2015;Rachniyom et al 2018) (Table 4).…”

Section: Phylogenetic Analysis Of Selected Isolatesmentioning

confidence: 99%

Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia

et al. 2019

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Abstract. Syafitri WA, Ningsih F, Setyaningsih PP, Rachmania MK, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridzal W. 2019. Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a geothermal area in West Java, Indonesia. Biodiversitas 20: 1929-1938. In this study, we describe the screening for amylolytic activity of 17 thermophilic Actinobacteria isolates obtained from the soil of Cisolok geysers, a geothermal area in West Java, Indonesia. All isolates were screened for amylolytic activity by the starch-agar plate method at various temperatures. The results showed that all of isolates were able to grow at 45oC. The growth abilities of the isolates grown in ISP 1 medium varied at temperatures from 45 to 60oC. Fifteen of the 17 isolates showed amylolytic activity at 45oC, 13 showed such activity at 50oC, and four showed activity at 55oC. Only three isolates, designated SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4, showed growth and amylolytic activity at 60oC. These three isolates were selected for molecular identification. The nearly full-length of 16S rRNA gene sequences data showed that these three isolates have a similarity of 99.93-100% with Actinomadura keratinilytica WCC-2265T and of 98.74-98.91% with A. miaoliensis BC 44T-5T. Phylogenetic tree shows that all three isolates are clustered together in a monophyletic group with the type strain of A. keratinilytica WCC-2265T as their most closely related species, with 100% bootstrap support. Based on sequencing of the 16S rRNA gene, phylogenetic comparison, and phenotypic characterization, the three isolates were identified as A. keratinilytica.

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“…The fatty acid profiles of Actinomadura species are found to be mostly complex mixtures of hexadecanoic (C 16:0 ), 14-methylpentadecanoic (iso-C 16:0 ) and 10-methyloctadecanoic acid [4]. Actinomadura species have been isolated from diverse habitats such as soil [5–8], marine sources [9], plant tissues [10, 11] and bee hives [12], and are a known source of diverse bioactive secondary metabolites. Over the last three decades, different bioactive secondary metabolites have been reported, including polyether antibiotic [13], anthracycline [14], polyketide [15] and β-carboline [16].…”

Section: Introductionmentioning

confidence: 99%

Actinomadura rubteroloni sp. nov. and Actinomadura macrotermitis sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis

Benndorf

Martin

Küfner

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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The taxonomic positions of two novel aerobic, Gram-positive actinobacteria, designated strains RB29T and RB68T, were determined using a polyphasic approach. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of RB29T were identified as Actinomadura rayongensis DSM 102126T (99.2 % similarity) and Actinomadura atramentaria DSM 43919T (98.7 %), and for strain RB68T was Actinomadura hibisca DSM 44148T (98.3 %). Digital DNA–DNA hybridization (dDDH) between RB29T and its closest phylogenetic neighbours, A. rayongensis DSM 102126T and A. atramentaria DSM 43919T, resulted in similarity values of 53.2 % (50.6–55.9 %) and 26.4 % (24.1–28.9 %), respectively. Additionally, the average nucleotide identity (ANI) was 93.2 % (94.0 %) for A. rayongensis DSM 102126T and 82.3 % (78.9 %) for A. atramentaria DSM 43919T. dDDH analysis between strain RB68T and A. hibisca DSM 44148T gave a similarity value of 24.5 % (22.2–27.0 %). Both strains, RB29T and RB68T, revealed morphological characteristics and chemotaxonomic features typical for the genus Actinomadura , such as the presence of meso-diaminopimelic acid in the cell wall, galactose and glucose as major sugar components within whole-cell hydrolysates and the absence of mycolic acids. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. Predominant menaquinones were MK-9(H6) and MK-9(H8) for RB29T and MK-9(H4) and MK-9(H6) for RB68T. The main fatty acids were identified as 10-methyloctadecanoic acid (10-methyl C18:0), 14-methylpentadecanoic acid (iso-C16:0), hexadecanoic acid (C16:0) and cis-9-octadecanoic acid (C18 : 1 ω9c). Here, we propose two novel species of the genus Actinomadura : Actinomadura rubteroloni sp. nov. with the type strain RB29T (=CCUG 72668T=NRRL B-65537T) and Actinomadura macrotermitis sp. nov. with the type strain RB68T (=CCUG 72669T=NRRL B-65538T).

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Actinomadura syzygii sp. nov., an endophytic actinomycete isolated from the roots of a jambolan plum tree (Syzygium cumini L. Skeels)

Cited by 18 publications

References 42 publications

Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a Geothermal Area in West Java, Indonesia

Actinomadura rubteroloni sp. nov. and Actinomadura macrotermitis sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis

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