Actinomyces georgiae sp. nov., Actinomyces gerencseriae sp. nov., Designation of Two Genospecies of Actinomyces naeslundii, and Inclusion of A. naeslundii serotypes II and III and Actinomyces viscosus serotype II in A. naeslundii Genospecies 2

Johnson, John L.; Moore, L. V. H.; Kaneko, Beverly; Moore, W. E. C.

doi:10.1099/00207713-40-3-273

Cited by 153 publications

(144 citation statements)

References 27 publications

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“…Cells were lysed and saponified and the acids were extracted as described previously (5). A 2-bl portion of washed extract was chromatographed on a fused-silica capillary column with a model HP-5890A chromatograph (HewlettPackard Co., Palo Alto, Calif.) equipped with a flame ionization detector and a model HP-3392A integrator (HewlettPackard).…”

Section: Methodsmentioning

confidence: 99%

Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; Hallella seregens gen. nov., sp. nov.; Johnsonella ignava gen. nov., sp. nov.; and Dialister pneumosintes gen. nov., comb. nov., nom. rev., Anaerobic Gram-Negative Bacilli from the Human Gingival Crevice

Moore

Moore²

1994

International Journal of Systematic Bacteriology

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111

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Many undescribed species of bacteria occur among the floras of the human gingival crevice and periodontal pockets (6,(8)(9)(10)(11)(12)(13)(14). Among these are organisms previously referred to as "Bacteroides D19" (9,11,12), "Bacteroides D26" (9, 14), and "Bacteroides D42" (9, 13), as well as one additional species ("Bacteroides D12") that we have not reported previously. We have found that "Bacteroides D19" is associated with gingivitis (11, 12) and periodontitis (9) and that "Bacteroides D42" is associated with periodontitis (9, 13), whereas "Bacteroides D26" is associated principally with oral health (9,14). One purpose of this study was to describe these four species.In addition, we propose reclassification of Bacteroides pneumosintes, a relatively common oral species that has been associated with gingivitis (1, 11, 12) and various infections above the diaphragm (3). This species is a small, non-fermentative, gram-negative, anaerobic rod, originally isolated from nasopharyngeal secretions of patients with influenza during the epidemics of 1918 through 1921 (15). In 1970 the species was reclassified in the genus Bacteroides by Holdeman and Moore (4). This nonfermentative species was excluded from the genus Bacteroides as redefined by Shah and Collins (16), and its taxonomic position currently is incertae sedis. MATERIALS AND METHODSBacterial strains. Unless otherwise stated, all strains were from our Virginia Polytechnic Institute and State University Anaerobe Laboratory Culture Collection and were isolated from the gingival crevices of clinically healthy persons or persons with gingivitis or periodontitis.Characterization of strains. Tests to determine fermentation, enzymatic properties, fermentation products, and antimicrobial susceptibility in prereduced media and polyacrylamide gel electrophoresis of soluble proteins were performed as described previously (2, 7).To determine cellular fatty acids, unless indicated otherwise, actively growing cultures were inoculated into tubes containing 10 ml of prereduced anaerobically sterilized peptone-yeast extract-glucose (PYG) broth (2). Cultures were inoculated under an oxygen-free carbon dioxide atmosphere or under an atmosphere containing an oxygen-free 10% carbon dioxide-90% nitrogen gas mixture. Tubes were stoppered with neoprene stoppers and were incubated at 37°C. All subsequent steps in sample preparation, including centrifugation, were done in the same tubes closed with Teflon-lined screw caps under an aerobic atmosphere.Sedimented cells from cultures that had been incubated at 37°C for 18 to 24 h were used immediately or were frozen and later thawed. Cells were lysed and saponified and the acids were extracted as described previously (5). A 2-bl portion of washed extract was chromatographed on a fused-silica capillary column with a model HP-5890A chromatograph (HewlettPackard Co., Palo Alto, Calif.) equipped with a flame ionization detector and a model HP-3392A integrator (HewlettPackard). The gas flow rates were ca. 400 ml/min for air, 30 ml/min for hydr...

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Section: Methodsmentioning

confidence: 99%

Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; Hallella seregens gen. nov., sp. nov.; Johnsonella ignava gen. nov., sp. nov.; and Dialister pneumosintes gen. nov., comb. nov., nom. rev., Anaerobic Gram-Negative Bacilli from the Human Gingival Crevice

Moore

Moore²

1994

International Journal of Systematic Bacteriology

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111

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“…Molecular chemical analysis and biochemical profiling also show the new bacterium is phenotypically distinct from all currently recognized species. Phenotypically the unknown organism most closely resembles Actinomyces viscosus serotype I (which includes the type strain of the species ;Johnson et al, 1990). However, it can be distinguished from Actinomyces viscosus serotype I in producing acid from melezitose.…”

mentioning

confidence: 99%

“…The results of neighbour-joining analysis showed Actinomyces viscosus serotype I, Actinomyces naeslundii and Actinomyces sp. serotype WVA 963 (Johnson et al, 1990) to be the nearest phylogenetic relatives of the unidentified bacterium (Fig. 2).…”

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confidence: 99%

Actinomyces bowdenii sp. nov., isolated from canine and feline clinical specimens

Pascual

Foster²,

Falsen

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

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Four strains of a previously undescribed Actinomyces-like bacterium were isolated from canine and feline clinical specimens. Phenotypic studies indicated the strains were members of the genus Actinomyces, and most closely resembled Actinomyces viscosus serotype I and Actinomyces slackii. Comparative 16s rRNA gene sequencing studies demonstrated the unknown bacterium constitutes a new subline within a group of Actinomyces species, which includes Actinomyces bovis, the type species of the genus. Based on phylogenetic and phenotypic evidence it is proposed that the unknown bacterium be classified as Actinomyces bowdenii sp. nov. The type strain of Actinomyces bowdenii is CCUG 3742IT.

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“…Actinomyces odontolyticus, Actinomyces naeslundii, and Actinomyces viscosus are the primary Actinomyces species in infants' mouths (21) as well as in early dental plaque (13,17). Actinomyces georgiae, Actinomyces gerensceriae, and Actinomyces meyeri have been isolated from gingival crevices of periodontally healthy individuals (3,10). Two new Actinomyces species of oral origin have been described recently: Actinomyces radicidentis from infected root canals (4) and Actinomyces graevenitzii from respiratory tract secretions (19) and infants' saliva (21).…”

mentioning

confidence: 99%

Phenotypic Identification of Actinomyces and Related Species Isolated from Human Sources

et al. 2001

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Recent advancements in chemotaxonomic and molecular biology-based identification methods have clarified the taxonomy of the genus Actinomyces and have led to the recognition of several new Actinomyces and related species. Actinomyces-like gram-positive rods have increasingly been isolated from various clinical specimens. Thus, an easily accessible scheme for reliable differentiation at the species level is needed in clinical and oral microbiology laboratories, where bacterial identification is mainly based on conventional biochemical methods. In the present study we designed a two-step protocol that consists of a flowchart that describes rapid, cost-efficient tests for preliminary identification of Actinomyces and closely related species and an updated more comprehensive scheme that also uses fermentation reactions for accurate differentiation of Actinomyces and closely related species.

show abstract

Actinomyces georgiae sp. nov., Actinomyces gerencseriae sp. nov., Designation of Two Genospecies of Actinomyces naeslundii, and Inclusion of A. naeslundii serotypes II and III and Actinomyces viscosus serotype II in A. naeslundii Genospecies 2

Cited by 153 publications

References 27 publications

Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; Hallella seregens gen. nov., sp. nov.; Johnsonella ignava gen. nov., sp. nov.; and Dialister pneumosintes gen. nov., comb. nov., nom. rev., Anaerobic Gram-Negative Bacilli from the Human Gingival Crevice

Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; Hallella seregens gen. nov., sp. nov.; Johnsonella ignava gen. nov., sp. nov.; and Dialister pneumosintes gen. nov., comb. nov., nom. rev., Anaerobic Gram-Negative Bacilli from the Human Gingival Crevice

Actinomyces bowdenii sp. nov., isolated from canine and feline clinical specimens

Phenotypic Identification of Actinomyces and Related Species Isolated from Human Sources

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