Actinomyces succiniciruminis sp. nov. and Actinomyces glycerinitolerans sp. nov., two novel organic acid-producing bacteria isolated from rumen

Abstract

Two bacterial strains, Am4 and G10 were isolated from rumen fluid of different ruminants: cow (Holstein-Friesian) and sheep (Slovenskè merino), respectively. They were isolated from different hosts and regions, but showed 99.2% similarity of the 16S rRNA genes. Both strains are versatile and ferment various sugars to mainly succinate and lactate and small amounts of acetate and formate. The 16S rRNA sequences of Am4 and G10 revealed that they belonged to the genus Actinomyces, and are related to Actinomyces ruminicola JCM 13352 with 97.0% and 97.4% similarity, respectively. DDH showed strain Am4 and G10 had only 55.8 and 43.3% similarity with the Actinomyces ruminicola JCM 13352, and had 69.9% similarity among each other. Comparing strain Am4 and G10, gANI value and dDDH were 92.9% and 68.6%, respectively. Additionally, AAI between the strains was 95.8%. MLSA of housekeeping genes showed difference of metG and pheS. The G+C% contents of strain Am4 and G10 were 69.8% and 68.5%, respectively. MK-10(H) was the principal quinone for strain Am4 (82%) and G10 (91%) with small amounts of MK-10(H) and MK-10(H) for both strains. Only MK-9(H) was detected in strain Am4. MALDI-TOF analysis of protein profiles also revealed that Am4 and G10 are different from each other and from Actinomyces ruminicola JCM 13352. Based on phylogenetic and physiological characteristics, together with genome comparison and MLSA we propose two novel species in the genus Actinomyces: Actinomyces succiniciruminis sp. nov. (type strain Am4=TISTR 2317=DSM 10376) and Actinomyces glycerinitolerans sp. nov. (type strain G10=TISTR 2318=DSM 10377).

Microbial Diversity and Organic Acid Production of Guinea Pig Faecal Samples

“…A bicarbonate-buffered anaerobic medium (BM) used in this experiment was prepared and supplemented as previously described [26, 28]. Then, 0.5% (w/v) of each substrate was added into serum bottles (duplicate bottles for each enrichment) before autoclaving.…”

Draft Genome Sequence of Actinomyces succiniciruminis Strain Am4 T , Isolated from Cow Rumen Fluid

“…Here, we report the draft genome sequence of a species belonging to the genus Actinomyces that originates from a cow rumen. Actinomyces succiniciruminis (Am4 T ) was isolated from an enrichment of amylopectin using rumen microorganisms (1). …”

“…This genus currently contains 47 species and two subspecies that are Gram-positive, pleomorphic, nonmotile, anaerobic, and aerotolerant bacteria with high G+C content (2). Actinomyces succiniciruminis can convert amylopectin, starch, and starch waste to mainly succinate, lactate, and small amounts of acetate and formate (1). …”

“…Genomic DNA of strain Am4 T was extracted from glucose grown cells (1) using the MasterPure complete DNA and RNA purification kit (Epicenter, Madison, WI). Genome sequencing was performed on an Illumina MiSeq sequencer with a read length of 250 bp and an insert size of 500 at GATC-Biotech, Konstanz, Germany.…”

Actinomyces wuliandei sp. nov., Corynebacterium liangguodongii sp. nov., Corynebacterium yudongzhengii sp. nov. and Oceanobacillus zhaokaii sp. nov., isolated from faeces of Tibetan antelope in the Qinghai-Tibet plateau of China

“…Since the initial description more than a century ago [1] and recent revision with the help of genome-scale data [2], the genus Actinomyces is now a large group of Gram-stain-positive, non-spore-forming, rod-shaped bacteria with high DNA G+C content [3,4]. Members of Actinomyces are widely distributed in nature such as in the intestinal mucous membranes of humans and animals [5], and some of them are potential pathogens [6]. In previous studies, we have isolated several novel species of the genus Actinomyces from wild animals [7][8][9][10].…”