Actinomycetes from marine habitats and their enzymatic potential

Singh, Satya P.; Thumar, Jignasha T.; Gohel, Sangeeta D.; Kikani, Bhavtosh A.; Shukla, Rushit J.; Sharma, Amit Kumar; Dangar, Kruti G.

doi:10.1533/9781908818355.2.191

Cited by 13 publications

(9 citation statements)

References 111 publications

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“…The seafloor covers about 70% of the Earth's surface and is carpeted by marine sediments, which are mixtures of complex organic and inorganic particles that accumulated due to accretion and erosion of the continents, oceanic biological activities, volcanic eruptions, and chemical processes within the ocean [1,2]. Given their vast coverage, marine sediments harbor remarkable diverse microbial communities accounting for 12-45% of the total microbial biomass or~0.6-2% of the whole biosphere [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Marine Sediment-Derived Streptomyces Strain Produces Angucycline Antibiotics against Multidrug-Resistant Staphylococcus aureus Harboring SCCmec Type 1 Gene

Sabido

Tenebro

Suarez

et al. 2020

JMSE

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The Philippine archipelago is geographically positioned in the tropics with rich areas of marine biodiversity. Its marine sediments harbor actinomycetes that exhibit antibacterial activity. Screening of actinomycetes isolated from marine sediments collected near the coast of Islas de Gigantes, Iloilo showed one isolate that exhibited high activity against the multidrug-resistant Staphylococcus aureus (MRSA) strain carrying the Staphylococcal Cassette Chromosome mec (SCCmec) type 1 gene, a biomarker for drug resistance. The isolate was identified as Streptomyces sp. strain DSD011 based on its 16s rRNA and protein-coding genes (atpD, recA, rpoB, and trpB) sequences, and was found to be a new species of salt-tolerant marine Streptomyces. Further, the strain harbors both non-ribosomal peptide synthetase (NRPS) and type II polyketide synthase (PKS) in its genome. The targeted chromatographic isolation and chemical investigations by Liquid Chromatography Mass Spectrometry-Time of Flight (LCMS-TOF), tandem mass spectrometry (MS/MS), and Global Natural Product Social molecular networking (GNPS) of the antibiotics produced by the strain, afforded the two polycyclic aromatic polyketide angucycline glycosides, fridamycin A (1) and fridamycin D (2), which are products of type II PKS biosynthesis. Compounds 1 and 2 displayed antibacterial activity against MRSA with minimum inhibitory concentration(MIC) of 500 μg/mL and 62.5 μg/mL, respectively. These results suggest that the underexplored marine sediments near the coast of Islas de Gigantes, Iloilo offer access to undiscovered Streptomyces species that are invaluable sources of antibiotic leads.

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Section: Introductionmentioning

confidence: 99%

Marine Sediment-Derived Streptomyces Strain Produces Angucycline Antibiotics against Multidrug-Resistant Staphylococcus aureus Harboring SCCmec Type 1 Gene

Sabido

Tenebro

Suarez

et al. 2020

JMSE

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“…1 Hence marine microbes offer novel biocatalysis and value added molecules. 2 Marine microbial diversity enacts limitless pool of novel metabolite chemistry that contributed an important source to the innovative biotechnology. 3 The search for novel antibiotics from the terrestrial microbes have diminished since this resource has been extensively explored and precious compounds have been already derived from these microbes.…”

Section: Introductionmentioning

confidence: 99%

Improved Bioactive Metabolite Production by Saccharopolyspora halotolerans VSM-2 Using Response Surface Methodology and Unstructured Kinetic Modelling

Managamuri¹,

Vijayalakshmi²,

Indupalli³

et al. 2018

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Background:This study targets to optimize and analyse the interactive effects of process variables for improved bioactive metabolite production using RSM and unstructured kinetic modelling by S. halotolerans VSM 2. Materials and Methods: RSM was applied to optimize the interactive effects of five variables, viz., time of incubation, pH, temperature, concentration of maltose and meat extract on bioactive metabolite production and its effect against the five responses viz., S. flexneri, S. marcescens, P. vulgaris, P. aeruginosa and E. coli. Models of Logistic and Luedeking-Piret were used to simulate the cellular increase and bioactive metabolite production. Results: RSM optimal conditions for the bioactive metabolite production recorded were incubation time (12days), pH (8), and temperature (25 0 C), concentrations of maltose and meat extract (1 % w/v) (each). The effect of the bioactive metabolite produced (zone of inhibition) against the responses were found to be 17 mm for S. flexneri, 17 mm for S. marcescens, 16 mm for P. vulgaris, 17 mm for P. aeruginosa and 18 mm for E coli. The data obtained from experimental values are in close agreement with the predicted values of RSM. Model adequacy was evaluated using ANOVA variance where the quadratic effect of p<0.0001 which imply the significance of the model. The unstructured-, mathematical-kinetic models provided a better approximation of profiles of S. halotolerans VSM 2 growth, optimized media utilization and bioactive metabolite production. Conclusion: Optimization of the independent variables for the production of the bioactive metabolite using RSM by S. halotolerans VSM 2 and its effect against the five responses were documented. The predicted values are in good agreement with the experimental values. Unstructured models provided a better approximation of kinetic profiles for bioactive metabolite production by S. halotolerans VSM 2.

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“…Actinobacteria have gained significance not only due to their taxonomy, diversity and ecological significance, but also on account of their enzymes, unique metabolites and bioactive compounds [1], [2].…”

Section: Introductionmentioning

confidence: 99%

“…Studies on the marine actinobacteria in recent years have emerged as one of the major aspects of research [1] . Actinobacteria have gained significance not only due to their taxonomy, diversity and ecological significance, but also on account of their enzymes, unique metabolites and bioactive compounds [1] , [2] .…”

Section: Introductionmentioning

confidence: 99%

“…However, studies on the salt tolerant haloalkaliphilic actinobacteria are quite rare. Haloalkaliphilic actinomycetes and their proteases from the saline habitats of the coastal Gujarat have been studied during the last several years [2] , [10] , [11] , [26] , [27] , [28] , [29] . In the present report, we describe the effect of amino acids on the production of alkaline protease in a newly isolated marine actinomycete, Nocardiopsis dassonvillei OK-18 from the Okha Port (22.4667°N 69.0833°E), Arabian Sea.…”

Section: Introductionmentioning

confidence: 99%

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Actinomycetes from marine habitats and their enzymatic potential

Cited by 13 publications

References 111 publications

Marine Sediment-Derived Streptomyces Strain Produces Angucycline Antibiotics against Multidrug-Resistant Staphylococcus aureus Harboring SCCmec Type 1 Gene

Marine Sediment-Derived Streptomyces Strain Produces Angucycline Antibiotics against Multidrug-Resistant Staphylococcus aureus Harboring SCCmec Type 1 Gene

Improved Bioactive Metabolite Production by Saccharopolyspora halotolerans VSM-2 Using Response Surface Methodology and Unstructured Kinetic Modelling

Effect of amino acids on the repression of alkaline protease synthesis in haloalkaliphilic Nocardiopsis dassonvillei

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