Actinomycin D: a novel Pseudomonas aeruginosa quorum sensing inhibitor from the endophyte Streptomyces cyaneochromogenes RC1

Zeng, Yue-Xiang; Liu, Junsheng; Wang, Yingjie; Tang, Shi; Wang, Dayong; Deng, Shi-Ming; Jia, Ai‐Qun

doi:10.1007/s11274-022-03360-y

Cited by 29 publications

(13 citation statements)

References 55 publications

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“…Actinomycin D inhibited acute myeloid leukemia (AML) through targeting of an oncogenic mutant form of the nucleolar chaperone nucleophosmin 1 [ 35 ]. Actinomycin D, a potent anti-virulence agent, is a promising candidate to treat Pseudomonas aeruginosa infection by interfering with the quorum sensing (QS) systems [ 36 ]. One strain Streptomyces globisporus isolated from the intestinal tract of Periplaneta americana , produced actinomycin X2 and collismycin A which showed strong inhibition of MRSA.…”

Section: Discussionmentioning

confidence: 99%

Anti-Klebsiella pneumoniae activity of secondary metabolism of Achromobacter from the intestine of Periplaneta americana

Ping

Chen

et al. 2023

BMC Microbiol

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Background Klebsiella pneumoniae is one of the main pathogens of clinical isolation and nosocomial infections, as K. pneumoniae show broad-spectrum resistance to β-lactam and carbapenem antibiotics. It is emerging clinical need for a safe and effective drug to anti-K. pneumoniae. At present, Achromobacter mainly focused on its degradation of petroleum hydrocarbons, polycyclic aromatic hydrocarbons, assisting insects to decompose, degrade heavy metals and utilize organic matter, but there were few reports on the antibacterial activity of the secondary metabolites of Achromobacter. Results In this study, a strain WA5-4-31 from the intestinal tract of Periplaneta americana exhibited strong activity against K. Pneumoniae through preliminary screening. The strain was determined to be Achromobacter sp. through the morphological characteristics, genotyping and phylogenetic tree analysis, which is homologous to Achromobacter ruhlandii by 99%, its accession numbe in GenBank at National Center for Biotechnology Information (NCBI) is MN007235, and its deposit number was GDMCC NO.1.2520. Six compounds (Actinomycin D, Actinomycin X2, Collismycin A, Citrinin, Neoechinulin A and Cytochalasin E) were isolated and determined by activity tracking, chemical separation, nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis. Among them, Actinomycin D, Actinomycin X2, Collismycin A, Citrinin and Cytochalasin E showed a good effect on anti-K. pneumoniae, with MIC values of 16–64 µg/mL. Conclusions The study reported Achromobacter, which was from the intestinal tract of Periplaneta americana with the activity against K. Pneumoniae, can produce antibacterial compounds for the first time. It lays the foundation for development of secondary metabolites of insect intestinal microorganisms.

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Section: Discussionmentioning

confidence: 99%

Anti-Klebsiella pneumoniae activity of secondary metabolism of Achromobacter from the intestine of Periplaneta americana

Ping

Chen

et al. 2023

BMC Microbiol

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“…The endophyte Streptomyces cyaneochromogenes RC1 was the source of actinomycin D, which loosened biofilm structures and weakened motility and other virulence factors in P. aeruginosa PAO1. Real-time PCR indicated the downregulation of major QS gene expression as pilA , pqsR , lasI , rhlI, pslA, and rhlR and the dramatic reduction in signaling metabolites of N-butanoyl-L-homoserine lactone and N-(3-oxododecanoyl)-L-homoserine lactone (Zeng et al 2022 ). The uropathogen Serratia marcescens was found to be susceptible to gentamicin after its combination with phenol, 2,4-bis(1,1-dimethylethyl).…”

Section: Secondary Metabolites From Endophytic Bacteriamentioning

confidence: 99%

“… Inhibited staphyloxanthin production in S. aureus (32.4%), inhibited bacterial transcription/translation (70.0%) Inhibiting the virulence and growth of S. aureus Arora et al ( 2016 ) 36 Thioketopiperazine 2 Glycyrrhiza glabra Phoma sp. Inhibited staphyloxanthin production in S. aureus (42.1%), inhibited bacterial transcription/translation (90%) Inhibiting the virulence and growth of S. aureus Arora et al ( 2016 ) 37 Actinomycin D Areca catechu L Streptomyces cyaneochromogenes RC1 inhibition of motility and virulence factors as rhamnolipid, pyocyanin, siderophores, and protease Reduced the virulence factors and biofilm formation of P. aeruginosa PAO1 Zeng et al ( 2022 ) 38 1-(4-Amino-2-hydroxyphenyl)ethanone Leaves of Punica granatum Phomopsis liquidambari S47 Reduced the secretion of acyl-homoserine lactones Attenuated the virulence of P. aeruginosa PAO1 Zhou et al ( 2021 ) 39 Mixture of F.A: palmitic, arachidic, stearic, oleic, and linolenic fatty acids Coriandrum sativum leaves Arthrographis kalrae Inhibited water insoluble EPS production Inhibition of S. mutans biofilms at conc of 31 μg/mL Abdel-Aziz et al ( 2020 ) 40 Nor-harmane Leaves of Orychophragmus violaceus Irpex lacteus Reduced fimbriae production Suppressed S. marcescens NJ01 virulence factors Luo et al ( 2021 ); Lee et al ( 2017 ) 41 ...…”

Section: Secondary Metabolites From Endophytic Bacteriamentioning

confidence: 99%

Endophytes: a uniquely tailored source of potential antibiotic adjuvants

Moussa

2024

Arch Microbiol

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Multidrug microbial resistance is risking an annual loss of more than 10 million people’ lives by 2050. Solutions include the rational use of antibiotics and the use of drugs that reduce resistance or completely obliterate them. Here endophytes come to play due to their high-yield production and inherent nature to produce antimicrobial molecules. Around 40%, 45% and 17% of antibacterial agents were obtained from fungi, actinomycetes, and bacteria, respectively, whose secondary metabolites revealed effectiveness against resistant microbes such as MRSA, MRSE, and Shigella flexneri. Endophyte’s role was not confined to bactericidal effect but extended to other mechanisms against MDR microbes, among which was the adjuvant role or the “magic bullets". Scarce focus was given to antibiotic adjuvants, and many laboratories today just screen for the antimicrobial activity without considering combinations with traditional antibiotics, which means real loss of promising resistance combating molecules. While some examples of synthetic adjuvants were introduced in the last decade, the number is still far from covering the disused antibiotics and restoring them back to clinical use. The data compiled in this article demonstrated the significance of quorum sensing as a foreseen mechanism for adjuvants from endophytes secondary metabolites, which call for urgent in-depth studies of their molecular mechanisms. This review, comprehensively and for the first time, sheds light on the significance of endophytes secondary metabolites in solving AMR problem as AB adjuvants.

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“…Further, the genomic DNA was extracted and amplified using ITS primers (5 ′ -TCCGTAGGTGGAACCTGGG-3 ′ and 5 ′ -TCTCCGCTTATTGCT-3 ′ ) and the obtained sequence was aligned in the NCBI. Based on the sequence similarities, the phylogenetic tree was constructed by the neighbor-joining method with the 1000-bootstrap value using MEGA 7.0 software [46]. The W2-F1 strain was identified as P. sacchari.…”

Section: Identification Of Qsi Active-strain W2-f1mentioning

confidence: 99%

An Investigation of Quorum Sensing Inhibitors against Bacillus cereus in The Endophytic Fungus Pithomyces sacchari of the Laurencia sp.

Xiang,

Xu,

Yin

et al. 2024

Marine Drugs

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Bacillus cereus, a common food-borne pathogen, forms biofilms and generates virulence factors through a quorum sensing (QS) mechanism. In this study, six compounds (dankasterone A, demethylincisterol A3, zinnimidine, cyclo-(L-Val-L-Pro), cyclo-(L-Ile-L-Pro), and cyclo-(L-Leu-L-Pro)) were isolated from the endophytic fungus Pithomyces sacchari of the Laurencia sp. in the South China Sea. Among them, demethylincisterol A3, a sterol derivative, exhibited strong QS inhibitory activity against B. cereus. The QS inhibitory activity of demethylincisterol A3 was evaluated through experiments. The minimum inhibitory concentration (MIC) of demethylincisterol A3 against B. cereus was 6.25 μg/mL. At sub-MIC concentrations, it significantly decreased biofilm formation, hindered mobility, and diminished the production of protease and hemolysin activity. Moreover, RT-qPCR results demonstrated that demethylincisterol A3 markedly inhibited the expression of QS-related genes (plcR and papR) in B. cereus. The exposure to demethylincisterol A3 resulted in the downregulation of genes (comER, tasA, rpoN, sinR, codY, nheA, hblD, and cytK) associated with biofilm formation, mobility, and virulence factors. Hence, demethylincisterol A3 is a potentially effective compound in the pipeline of innovative antimicrobial therapies.

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Actinomycin D: a novel Pseudomonas aeruginosa quorum sensing inhibitor from the endophyte Streptomyces cyaneochromogenes RC1

Cited by 29 publications

References 55 publications

Anti-Klebsiella pneumoniae activity of secondary metabolism of Achromobacter from the intestine of Periplaneta americana

Anti-Klebsiella pneumoniae activity of secondary metabolism of Achromobacter from the intestine of Periplaneta americana

Endophytes: a uniquely tailored source of potential antibiotic adjuvants

An Investigation of Quorum Sensing Inhibitors against Bacillus cereus in The Endophytic Fungus Pithomyces sacchari of the Laurencia sp.

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