Actinomycin D inhibits hemoglobin synthesis in rabbit reticulocyte lysates and binds to a 15 S cytoplasmic particle involved in the initiation step of translation

Felicetti, L.; Urbani, Claudio

doi:10.1016/0005-2787(77)90079-x

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Cycloheximide (CHM) inhibits protein synthesis (Ennis and Lubin 1964, Brown et al 1970, Jones 1977. Actinomycin D (AMD) inhibits DNA-dependent RNA synthesis (Goldberg andRabinowitz 1962, Felicetti andUrbani 1977).…”

Section: Time and Duration Of Amd Applicationmentioning

confidence: 99%

Influence of Cycloheximide and Actinomycin D on Initiation and Early Development of Adventitious Roots

Oppenoorth¹

1979

Physiologia Plantarum

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From leaf cuttings of the bean Phaseolus vulgaris L. adventitious roots form on the petiole. This root formation is stimulated by treatment with auxin. Simultaneous or subsequent application of cycloheximide irreversibly inhibited dedifferentiation, so that root production was completely prevented. The effects of actinomycin D application depended upon the stage of development of the root primordium. Cells in the first stage of dedifferentiation were extremely sensitive. When actinomycin D was applied later than 6 h after cutting, its inhibiting effect gradually diminished. It is concluded that an actinomycin D‐sensitive process occurring early in dedifferentiation is crucial for root initiation. A second, less actinomycin D‐sensitive process occurring later in dedifferentiation is required for the further development of the root primordium. During the initiation and development of the root primordium protein synthesis is required.

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Section: Time and Duration Of Amd Applicationmentioning

confidence: 99%

Influence of Cycloheximide and Actinomycin D on Initiation and Early Development of Adventitious Roots

Oppenoorth¹

1979

Physiologia Plantarum

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The Association of DNA and RNA with Membranes

Moyer

1979

International Review of Cytology

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Half‐Life of Histone H4 mRNA in Xenopus laevis Embryonic Cells at Different Stages

Atsuchi¹,

Tashiro²,

Fu³

et al. 1987

Dev Growth Differ

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By Northern blotting methods we have recently found that the relative amount of histone H4 mRNA per Xenopus embryo is constant during cleavage, doubles during the gastrula stage, then decreases at the neurula stage to the level slightly lower than that of the cleavage-embryo. At each developmental stage, the level of mRNA will depend on three parameters: amount of maternal mRNA remaining, rate of the synthesis of the mRNA and rate of the decay of the mRNA. In the present experiment, we compared rates of the decay of H4 mRNA in Xenopus embryonic cells at different stages by pulse-chase experiments with actinomycin D as an inhibitor of transcription.Under the conditions used, the half-life of H4 mRNA was estimated to be 90,80, and 100 min, for the late blastula, late gastrula and neurula stages, respectively. The decay of H4 mRNA with a half-life of 90 min at the late blastula stage predicts exhaustion of most of maternal H4 mRNA by the early gastrula stage. The slightly longer half-life of H4 mRNA in neurula than in late gastrula cells suggests that H4 mRNA is more stable in neurula cells than in late gastrula cells, and therefore, the large decrease in the level of H4 mRNA observed at the neurula stage does not depend on the increase in the turnover-rate of H4 mRNA. Probably, neurula cells synthesize less H4 mRNA than late gastrula cells but utilize it for longer time.Amphibian eggs and zygotes contain large amounts of maternal mRNAs. Therefore, the synthesis of proteins during very early stages of development appears to be regulated mainly at the translational level, and it is only later in the development that embryos rely their protein synthesis on new transcription from zygotic genomes (1).Histone mRNA is the first example of maternal mRNAs, that was discovered in sea urchin eggs (2, 3). In Xenopus embryos also, histone mRNA is probably one of the best known examples of maternal mRNAs (4): Several studies have been carried out by analyzing the products of in vitro translation of histone-synthesizing mRNAs (5-7), and by hybridization with specific histone mRNA-probes as in the case of HI (8-10) and H2 and H3 (11).In our recent studies on the expression of histone H4 gene, we have found that the activity of the expression of this gene does not depend on the occurrence of cell adhesion (12). In this experiment we noticed that the amount per embryo of this mRNA greatly differs between the gastrula and neurula stages: Its amount was fairly constant during cleavage, but almost doubled at the gastrula stage, and then decreased at the neurula stage to the level Present address:

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Actinomycin D inhibits hemoglobin synthesis in rabbit reticulocyte lysates and binds to a 15 S cytoplasmic particle involved in the initiation step of translation

Cited by 3 publications

References 22 publications

Influence of Cycloheximide and Actinomycin D on Initiation and Early Development of Adventitious Roots

Influence of Cycloheximide and Actinomycin D on Initiation and Early Development of Adventitious Roots

The Association of DNA and RNA with Membranes

Half‐Life of Histone H4 mRNA in Xenopus laevis Embryonic Cells at Different Stages

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