Actinophytocola algeriensis sp. nov., an actinobacterium isolated from Saharan soil

Abstract

During our investigations of new actinobacterial taxa, a novel actinobacterial strain, designated MB20 T , was isolated from a Saharan soil sample, collected in the Mzab region (Ghardaïa province, southern Algeria). In order to reveal its taxonomic position, the novel strain was characterized following a polyphasic taxonomic approach. It was noticed that strain MB20 T produced white, branched and fragmented substrate mycelium with no aerial mycelium on most of the media tested. Chemotaxonomic and phylogenetic studies clearly demonstrated that strain MB20 T belonged to the family Pseudonocardiaceae and was closely related to the genus Actinophytocola. Cell-wall hydrolysates contained meso-diaminopimelic acid but not glycine, and whole-cell hydrolysates contained galactose, glucose and ribose. The diagnostic phospholipid was phosphatidylethanolamine. Mycolic acids were not detected while the predominant fatty acid was found to be iso-branched hexadecanoate (iso-C 16 : 0 ). The major menaquinone was MK-9 (H 4 ). Results of the 16S rRNA gene sequence comparison revealed that strain MB20 T shared the highest degree of similarity with Actinophytocola gilvus DSM 45828 T (98.5 %), Actinophytocola corallina DSM 45659 T (98.0 %) and Actinophytocola timorensis DSM 45660 T (97.5 %). However, DNA-DNA hybridization studies showed only 32.9 % similarity with A. timorensis, 23.7 % similarity with A. gilvus and 17.9 % similarity with A. corallina. On the basis of phenotypic characteristics, 16S rRNA gene sequence comparisons and DNA-DNA hybridization, strain MB20 T was revealed to be a representative of a novel species of the genus Actinophytocola, for which the name Actinophytocola algeriensis sp. nov. (type strain MB20 T =DSM 46746 T =CECT 8960 T ) is proposed.

Coupling the endophytic microbiome with the host transcriptome in olive roots

“…In addition, these connections were stronger (lower GD and M) than in the AL network ( Table 4 ). Considering the predominance of Actinophytocola in the AH group and the precedents on the adaptive capabilities of this genus to water stress conditions [50] , [51] , [53] , the topological parameters found in this network suggested that the communities of each group responded to water stress (at least under the conditions found in the orchard at the time of sampling) in a very different way. In AH cultivars, the whole community seemed to work coordinately to adapt quickly to those stressing conditions.…”

“…Indeed, representatives of Actinophytocola spp. have been found in highly arid environments like the Sahara, Tenger Desert (China) and Mongolia [50] , [51] , [52] , [53] . Interestingly, Actinophytocola was not only isolated from soils but also from inside the root of Thai glutinous rice plants ( Oryza sativa sp.)…”

Nitrate determines the bacterial habitat specialization and impacts microbial functions in a subsurface karst cave

“…Wb1-P19 is a genus named after an uncultured clone in cave water, which phylogenetically clusters with sulfur- or nitrite-oxidizing autotrophs ( Holmes et al, 2001 ). Species in Conexibacter , Sphingomonas , Kribbella , Nocardioides , Amycolatopsis , Streptomyces , Actinophytocola , Aeromicrobium , Stackebrandtia , and Rubrobacter can reduce nitrate into nitrite ( Pranamuda et al, 1997 ; Monciardini et al, 2003 ; Keulen et al, 2005 ; Labeda and Kroppenstedt, 2005 ; Prakash and Lal, 2006 ; Ramasamy et al, 2012 ; Seki et al, 2012 ; Suzuki, 2015 ; Bouznada et al, 2016 ; Guerrero-Cruz et al, 2019 ; Curtis et al, 2020 ). Nitrosospira is a ubiquitous ammonium-oxidizing bacterium (AOB) found in various environments ( Norton et al, 2008 ).…”

New Glycosylated Polyene Macrolides: Refining the Ore from Genome Mining

“…Actinophytocola algeriensis was isolated from a Saharan soil sample from Southern Algeria [124]. The genome contains a BGC encoding a complete 17-module PKS for a tetraene with a side-chain (Figure 30; Supplementary Materials Figures S23 and S24).…”