Actinoplanes lutulentus sp. nov., isolated from mucky soil in China

Abstract

A novel actinomycete, designated strain NEAU-GRX6 T , was isolated from mucky soil collected from a stream of Jinlong Mountain in Harbin, Heilongjiang Province, north China, and characterized using a polyphasic approach. The isolate formed irregular sporangia containing motile sporangiospores on the substrate mycelium. The whole-cell sugars were xylose, glucose and galactose. The predominant menaquinones were MK-9(H 6 ), ) and MK-9(H 4 ). The major fatty acids were C 16 : 0 , C 15 : 0 , C 18 : 1 v9c, C 17 : 1 v7c and C 18 : 0 . The phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The DNA G+C content was 67 mol%. 16S rRNA gene sequence similarity studies showed that strain NEAU-GRX6T belonged to the genus Actinoplanes, being most closely related to Actinoplanes palleronii IFO 14916 T (97.80 % similarity) and Actinoplanes missouriensis NBRC 102363 T (97.76 %). However, the low observed levels of DNA-DNA relatedness allowed the isolate to be differentiated from the above-mentioned species of the genus Actinoplanes. Moreover, strain NEAU-GRX6 T could also be distinguished from A. palleronii IFO 14916 T and A.missouriensis NBRC 102363 T by phenotypic characteristics. Therefore, it is proposed that strain NEAU-GRX6 T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes lutulentus sp. nov. is proposed. The type strain is strain NEAU-GRX6 T (5CGMCC 4.7090The genus Actinoplanes, belonging to the family Micromonosporaceae, was described by Couch (1950). Actinoplanes philippinensis is the type species. Members of the genus develop spherical, cylindrical, digitate, lobate, bottle-or flaskshaped or very irregular sporangia that contain motile sporangiospores with tufts of polar flagella at the ends of sporangiophores on the substrate mycelium. The first detailed phenotypic and chemotaxonomic analysis of the genus was provided by Goodfellow et al. (1990), who determined the chemotaxonomic and phenotypic characteristics of species of the genus Actinoplanes and reported that chemical and numerical taxonomic data supported the integrity of the genus. A comprehensive phylogenetic analysis of the genus has been given by Tamura & Hatano (2001). At the time of writing, the genus Actinoplanes comprised 36 species, including recently described 'Actinoplanes hulinensis ' (Shen et al., 2013) and Actinoplanes siamensis (Suriyachadkun et al., 2013). During the investigation of potential sources of novel species and novel natural products, strain NEAU-GRX6 T was isolated from mucky soil collected from a stream of Jinlong Mountain in Harbin, Heilongjiang Province, north China. In this study, the taxonomic status of this strain is reported based on phylogenetic, chemotaxonomic and phenotypic evidence. It is proposed that strain NEAU-GRX6T should be classified as representing a novel species of the genus Actinoplanes. Strain NEAU-GRX6T was isolated from mucky soil collected from a stream of Jinlong Mountain in Harbin, Heilongjiang Province,...

Actinoplanes oblitus sp. nov., producing the glycopeptide antibiotic A477

“…Tetra-hydrogenated menaquinone with nine isoprene units – MK-9(H 4 ) – is commonly the predominant isoprenologue followed by minor amounts of hexa- and octahydrogenated menaquinones with nine isoprene units isoprenologues – MK-9(H 6 ) and MK-9(H 8 ) [2]. Iso/anteiso branched and monounsaturated fatty acids and/or cis -9,10-octadecanoic acid are often present, though their proportions may vary considerably between the species [2, 7–10, 12, 13].…”

“…At the time of writing (September 2023), the genus Actinoplanes consisted of 53 species with validly published species names, as reported in the List of Prokaryotic names with Standing in Nomenclature (https://lpsn.dsmz.de/genus/actinoplanes), including recently published Actinoplanes ramoplaninifer [7], Actinoplanes deserti [8], Actinoplanes flavus [9], Actinoplanes aksuensis , Actinoplanes polyasparticus , Actinoplanes hotanensis [10], and Actinoplanes maris [14]. Actinoplanes inhabit various natural environments and were isolated from temperate soils [15], mucky soils [12], arid and desert soils [8, 16], marine sediments [17], and rhizospheres of different plants [9, 18] or lichens [19]. Their distribution in different biotopes indicates the ecological importance of these microorganisms, which are mostly known for their ability to produce clinically relevant (or promising) drugs, such as acarbose [20], teicoplanin [21], purpuromycin [22, 23], ramoplanin [24], and friulimycin [25].…”

Actinoplanes sandaracinus sp. nov., a novel ligninase- producing and cellulose-degrading actinobacterium isolated from soil

“…Furthermore, members of the genus develop sporangia that can be spherical, cylindrical, digitate, lobate, bottle or flask-shaped, or irregular, containing motile spores. Clumps of polar flagella can be observed at the tips of sporangiophores on the substrate mycelia [8]. During our study of novel rare actinobacteria from soil sample collected from Chifeng City, Inner Mongolia Autonomous Region, an Actinoplanes -like strain designated as NEAU-A12 T with ligninase-producing and cellulose-degrading ability was isolated.…”

Actinoplanes rhizophilus sp. nov., an actinomycete isolated from the rhizosphere of Sansevieria trifasciata Prain

“…The detailed phenotypic and chemotaxonomic analysis of the genus was first provided by Goodfellow et al (1990), and a comprehensive phylogenetic analysis of the genus was given by Tamura & Hatano (2001). At the time of writing, the genus Actinoplanes comprises 37 species with validly published names (http://www.bacterio.net/actinoplanes.html), including the recently described Actinoplanes nipponensis (Wink et al , 2014), Actinoplanes lutulentus (Gao et al , 2014a) and Actinoplanes siamensis (Suriyachadkun et al , 2013). During an investigation exploring potential sources of novel species and novel natural products, strain NEAU-A-2 T was isolated from rhizosphere soil of Sansevieria trifasciata Prain.…”