Actinorhabdospora filicis gen. nov., sp. nov., a new member of the family Micromonosporaceae

Abstract

The actinomycete strains K12-0408 T and K12-0792 were isolated on CM-cellulose agar from rhizosphere soil of a pteridophytic plant collected in Tokyo prefecture, Japan. Their taxonomic positions were determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains K12-0408 T and K12-0792 were positioned within the family Micromonosporaceae. The strains formed extensively branched aerial and substrate mycelia. Long chains of cylindrical spores with smooth surfaces were formed on aerial hyphae. The cell wall contained meso-diaminopimelic acid, and galactose, glucose, mannose and ribose were detected in whole-cell hydrolysates. The predominant menaquinones were MK-10(H 4 ) and MK-10(H 6 ). The major cellular fatty acids were anteiso-C 17 : 0 and anteiso-C 17 : 0 2-OH. The DNA G+C contents of strains K12-0408 T and K12-0792 were 69.6 and 69.7 mol%, respectively. Based on data from the present polyphasic taxonomic study, strains K12-0408 T and K12-0792 represent a novel genus, for which the name Actinorhabdospora gen. nov. is proposed, with strain K12-0408 T (=NBRC 111897 T =TBRC 5327 T ) as the type strain of the type species, Actinorhabdospora filicis sp. nov.The family Micromonosporaceae was first described by Krasil'nikov (1938) and the description was amended by Koch et al. (1996), Stackebrandt et al. (1997) and Zhi et al. (2009) on the basis of chemotaxonomic data and 16S rRNA gene sequence analysis. During the characterization of actinomycetes isolated from rhizospheric soil of fern plants, strains K12-0408 T and K12-0792 were recovered. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences indicated that the two strains were distinct from genera within the family Micromonosporaceae. Herein, we report the results of phenotypic, chemotaxonomic and genomic analyses of strains K12-0408 T and K12-0792, which suggested that the strains represented a novel species of a new genus.Strains K12-0408 T and K12-0792 were isolated from rhizosphere soil of ferns (pteridophyte plants) collected at Fussa and Hamura city, respectively, located in Tokyo prefecture, Japan. The CM-cellulose (CMC) medium used for the isolation procedure contained the following: 1.0 % CM-cellulose sodium salts, 0.1 % K 2 HPO 4 , 0.1 % L-asparagine monohydrate, 0.0001 % FeSO 4 Á7H 2 O, 0.0001 % MnCl 2 Á4H 2 O, 0.0001 % ZnSO 4 Á7H 2 O and 1.5 % agar (adjusted to pH 7.0±0.2 with 1 M NaOH). The medium was supplemented with 0.025 % nalidixic acid and 0.02 % Benlate (Dupont) to minimize and inhibit growth of unwanted bacteria and fungi, respectively. The pure cultures of strains K12-0408 T and K12-0792 were maintained on International Streptomyces Project (ISP) medium 2 (Shirling & Gottlieb, 1966) and in 20 % (v/v) glycerol suspension at À20 C. Biomass for taxonomic characterization was obtained from cultures grown at 27 C for 7 days in yeast extract-dextrose (YD) broth containing 1.0 % yeast extract and 1.0 % glucose.The morphological characteristics of strains K12-0408 T and K12-0792 ...

Genome-based classification of micromonosporae with a focus on their biotechnological and ecological potential

“…This study was designed to explore the extent to which whole genome sequence data derived from type strains of the genus Micromonospora can be used to clarify relationships within this taxon and provide insights into the biological properties and biotechnological potential of micromonosporae. The genus Micromonospora 44 is the type genus of the family Micromonosporaceae 45 of the order Micromonosporales 46 ; the family encompasses 31 validly named genera which can be distinguished using a combination of chemotaxonomic, morphological and phylogenetic criteria 45 , 47 . The genus was proposed by Ørskov in 1923 48 for strains isolated from air that had been designated as “ Streptothrix chalcea ” by Foulerton 49 and then reclassified as Micromonospora chalcea , the type species of the genus.…”

Metabarcoding analysis reveals an interaction among distinct groups of bacteria associated with three different varietals of grapes used for wine production in Brazil

“…This is the first study that observed a positive correlation of soil potassium and the genera Oikopleura , Actinorhabdospora , Enteractinococcus , Paenisporosarcina , Thiobacillus , Ornithinicoccus , Oleiagrimonas , Candidatus Caldatribacterium , Anseongella , Bhargavaea , Globicatella , Planococcus , OLB17 , Xenophilus and Mitsuaria . These groups of bacteria were largely found in soils of cultures other than grapevines, although no correlation analysis with nutrients have been previously shown [ [52] , [53] , [54] , [55] , [56] , [57] , [58] , [59] , [60] , [61] , [62] ]. Dietzia and Xenophilus have also been found in other studies on grapevine soil [ 63 , 64 ].…”