Action of a deproteinized xenogenic biomaterial in the process of bone repair in rats submitted to inhalation of cigarette smoke

Buchaim, Daniela Vieira; Bueno, Patrícia Cincotto dos Santos; Andreo, Jesús Carlos; Roque, Domingos Donizeti; Roque, José Sidney; Zilio, Marcelo Garcia; Salatin, Jefferson Augusto; Kawano, Natália; Furlanette, Gabriela

doi:10.1590/s0102-865020180040000004

Cited by 7 publications

(7 citation statements)

References 31 publications

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“…The distribution of animals in their respective groups in the present study, as well as the number of specimens (n), was based on the principle of the 3 R's, in which there is a commitment by the world scientific community to follow the Russell-Burch Principles (1959) "reduction, replacement and refinement" in the use of animals that, increasingly, remain active in scientific and academic circles. Therefore, it was decided to not perform groups with defects filled only by clot, autogenous bone [3,17] or Bio-Oss ® [5,7,28,45], widely previously published in the literature, including the same methodology used in the present experiment and also from the same research group [39,[46][47][48], focusing on only in the originality and aims of the research.…”

Section: Discussionmentioning

confidence: 99%

“…15 scalpel blade, a 20 mm long linear incision was made in the craniocaudal direction, sectioning the skin and muscle fasciae, reaching the periosteum and extending it for tibial exposure. With an AR 6 steel carbide drill (Beavers Dental ® , Morrisburg, ON, Canada, K0C 1 × 0/Ormex SA) coupled to a 1500 rpm low-speed micromotor, a 2 mm diameter cavity was prepared [31][32][33][34][35] in a depth reaching the bone marrow without damaging the contralateral cortical [18,[36][37][38][39], with abundant irrigation of 0.9% sodium chloride solution.…”

Section: Monocortical Defect Surgerymentioning

confidence: 99%

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Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones

et al. 2020

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Bone defects cause aesthetic and functional changes that affect the social, economic and especially the emotional life of human beings. This complication stimulates the scientific community to investigate strategies aimed at improving bone reconstruction processes using complementary therapies. Photobiomodulation therapy (PBMT) and the use of new biomaterials, including heterologous fibrin biopolymer (HFB), are included in this challenge. The objective of the present study was to evaluate the influence of photobiomodulation therapy on bone tibial reconstruction of rats with biomaterial consisting of lyophilized bovine bone matrix (BM) associated or not with heterologous fibrin biopolymer. Thirty male rats were randomly separated into three groups of 10 animals. In all animals, after the anesthetic procedure, a noncritical tibial defect of 2 mm was performed. The groups received the following treatments: Group 1: BM + PBMT, Group 2: BM + HFB and Group 3: BM + HFB + PBMT. The animals from Groups 1 and 3 were submitted to PBMT in the immediate postoperative period and every 48 h until the day of euthanasia that occurred at 14 and 42 days. Analyses by computed microtomography (µCT) and histomorphometry showed statistical difference in the percentage of bone formation between Groups 3 (BM + HB + PBMT) and 2 (BM + HFB) (26.4% ± 1.03% and 20.0% ± 1.87%, respectively) at 14 days and at 42 days (38.2% ± 1.59% and 31.6% ± 1.33%, respectively), and at 42 days there was presence of bone with mature characteristics and organized connective tissue. The µCT demonstrated BM particles filling the defect and the deposition of new bone in the superficial region, especially in the ruptured cortical. It was concluded that the association of PBMT with HFB and BM has the potential to assist in the process of reconstructing bone defects in the tibia of rats.

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Section: Discussionmentioning

confidence: 99%

Section: Monocortical Defect Surgerymentioning

confidence: 99%

Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones

et al. 2020

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“…There are currently available treatment options for the repair of bone defects, but their effectiveness is limited in large defects, and the influence of extrinsic factors such as smoking and alcohol exposure are unfavourable in this process [1,2,3]. Annually more than two million bone grafts are performed worldwide, the second most frequent tissue transplantation, being surpassed only by blood transfusion [4,5].…”

Section: Introductionmentioning

confidence: 99%

Fibrin Sealant Derived from Human Plasma as a Scaffold for Bone Grafts Associated with Photobiomodulation Therapy

Pomini

Buchaim

Andreo

et al. 2019

IJMS

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Fibrin sealants derived from human blood can be used in tissue engineering to assist in the repair of bone defects. The objective of this study was to evaluate the support system formed by a xenograft fibrin sealant associated with photobiomodulation therapy of critical defects in rat calvaria. Thirty-six rats were divided into four groups: BC (n = 8), defect filled with blood clot; FSB (n = 10), filled with fibrin sealant and xenograft; BCPBMT (n = 8), blood clot and photobiomodulation; FSBPBMT (n = 10), fibrin sealant, xenograft, and photobiomodulation. The animals were killed after 14 and 42 days. In the histological and microtomographic analysis, new bone formation was observed in all groups, limited to the defect margins, and without complete wound closure. In the FSB group, bone formation increased between periods (4.3 ± 0.46 to 6.01 ± 0.32), yet with lower volume density when compared to the FSBPBMT (5.6 ± 0.45 to 10.64 ± 0.97) group. It was concluded that the support system formed by the xenograft fibrin sealant associated with the photobiomodulation therapy protocol had a positive effect on the bone repair process.

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“…Biomaterials are traditionally classified according to their origin into synthetic or natural materials. Natural biomaterials for use as membranes can be autologous, such as platelet-rich fibrin [ 36 ], fibrin sealants [ 37 , 38 , 39 , 40 , 41 ], or xenogenous [ 42 , 43 , 44 ]. The three membranes used in the present study belong to the latter category.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Study of Nasal Bone Reconstruction with Collagen, Elastin and Chitosan Membranes in Abstainer and Alcoholic Rats

et al. 2022

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The aim of the present study was to evaluate the use of collagen, elastin, or chitosan biomaterial for bone reconstruction in rats submitted or not to experimental alcoholism. Wistar male rats were divided into eight groups, submitted to chronic alcohol ingestion (G5 to G8) or not (G1 to G4). Nasal bone defects were filled with clot in animals of G1 and G5 and with collagen, elastin, and chitosan grafts in G2/G6, G3/G7, and G4/G8, respectively. Six weeks after, all specimens underwent radiographic, tomographic, and microscopic evaluations. Bone mineral density was lower in the defect area in alcoholic animals compared to the abstainer animals. Bone neoformation was greater in the abstainer groups receiving the elastin membrane and in abstainer and alcoholic rats receiving the chitosan membrane (15.78 ± 1.19, 27.81 ± 0.91, 47.29 ± 0.97, 42.69 ± 1.52, 13.81 ± 1.60, 18.59 ± 1.37, 16.54 ± 0.89, and 37.06 ± 1.17 in G1 to G8, respectively). In conclusion, osteogenesis and bone density were more expressive after the application of the elastin matrix in abstainer animals and of the chitosan matrix in both abstainer and alcoholic animals. Chronic alcohol ingestion resulted in lower bone formation and greater formation of fibrous connective tissue.

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Action of a deproteinized xenogenic biomaterial in the process of bone repair in rats submitted to inhalation of cigarette smoke

Abstract: The bone matrix acted as an osteoinductive biomaterial, biocompatible and aided in the repair process, mainly in the initial period of recovery.

Cited by 7 publications

References 31 publications

Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones

Photobiomodulation Therapy Associated with Heterologous Fibrin Biopolymer and Bovine Bone Matrix Helps to Reconstruct Long Bones

Fibrin Sealant Derived from Human Plasma as a Scaffold for Bone Grafts Associated with Photobiomodulation Therapy

In Vivo Study of Nasal Bone Reconstruction with Collagen, Elastin and Chitosan Membranes in Abstainer and Alcoholic Rats

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