Action of a midcycle contraceptive (R 2323) on the human endometrium

Azadian-Boulanger, G.; Secchi, Jean; Laraque, F.; Raynaud, Jean‐Pierre; Sakiz, E

doi:10.1016/0002-9378(76)90807-3

Cited by 30 publications

(10 citation statements)

References 13 publications

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“…NCSs have strictly been observed post ovulation, only on cycle days 16-24, and are not detected in pregnancy (Clyman, 1963). They appear to be induced by progesterone and are sensitive to oral and intrauterine contraceptives (Azadian-Boulanger et al, 1976;FeriaVelasco et al, 1972;Kohorn et al, 1970;Kohorn et al, 1972;PryseDavies et al, 1979;Roberts et al, 1975;Wynn, 1967). Finally, in several cases of unexplained infertility the absence or delayed appearance of NCSs was noted as the sole abnormal endometrial parameter (Dockery et al, 1996;Gore and Gordon, 1974;Kohorn et al, 1972).…”

Section: Introductionmentioning

confidence: 99%

Nuclear pore complex proteins mark the implantation window in human endometrium

Guffanti

Kittur

Brodt

et al. 2008

Journal of Cell Science

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Nucleolar channel systems (NCSs) are membranous organelles appearing transiently in the epithelial cell nuclei of postovulatory human endometrium. Their characterization and use as markers for a healthy receptive endometrium have been limited because they are only identifiable by electron microscopy. Here we describe the light microscopic detection of NCSs using immunofluorescence. Specifically, the monoclonal nuclear pore complex antibody 414 shows that NCSs are present in about half of all human endometrial epithelial cells but not in any other cell type, tissue or species. Most nuclei contain only a single NCS of uniform 1 μm diameter indicating a tightly controlled organelle. The composition of NCSs is as unique as their structure; they contain only a subset each of the proteins of nuclear pore complexes, inner nuclear membrane, nuclear lamina and endoplasmic reticulum. Validation of our robust NCS detection method on 95 endometrial biopsies defines a 6-day window, days 19-24 (±1) of an idealized 28 day cycle, wherein NCSs occur. Therefore, NCSs precede and overlap with the implantation window and serve as potential markers of uterine receptivity. The immunodetection assay, combined with the hitherto underappreciated prevalence of NCSs, now enables simple screening and further molecular and functional dissection.

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Section: Introductionmentioning

confidence: 99%

Nuclear pore complex proteins mark the implantation window in human endometrium

Guffanti

Kittur

Brodt

et al. 2008

Journal of Cell Science

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“…This organelle appears during a 3-to 4-d window in the midsecretory phase of the menstrual cycle when the endometrium is receptive to implantation of the fertilized egg (Clyman, 1963;Gordon, 1975). Oral contraceptives and intrauterine devices interfere with the timing and/or overall appearance of the NCS (Wynn, 1967;Feria-Velasco et al, 1972;Azadian-Boulanger et al, 1976;van Santen et al, 1988;Dockery et al, 1997). Several cases of unexplained primary infertility have been characterized by the absence or delayed development of NCSs as the only ultrastructural alteration in endometrial epithelial cells (Kohorn et al, 1972;Gore and Gordon, 1974;Dockery et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

The Nucleolar Channel System of Human Endometrium Is Related to Endoplasmic Reticulum and R-Rings

Kittur¹,

Zapantis

Aubuchon

et al. 2007

MBoC

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The nucleolar channel system (NCS) is a well-established ultrastructural hallmark of the postovulation endometrium. Its transient presence has been associated with human fertility. Nevertheless, the biogenesis, composition, and function of these intranuclear membrane cisternae are unknown. Membrane systems with a striking ultrastructural resemblance to the NCS, termed R-rings, are induced in nuclei of tissue culture cells by overexpression of the central repeat domain of the nucleolar protein Nopp140. Here we provide a first molecular characterization of the NCS and compare the biogenesis of these two enigmatic organelles. Like the R-rings, the NCS consists of endoplasmic reticulum harboring the marker glucose-6-phosphatase. R-ring formation initiates at the nuclear envelope, apparently by a calcium-mediated Nopp140-membrane interaction, as supported by the calcium-binding ability of Nopp140, the inhibition of R-ring formation by calcium chelators, and the concentration of Nopp140 and complexed calcium in R-rings. Although biogenesis of the NCS may initiate similarly, the reduced presence of complexed calcium and Nopp140 suggests the involvement of additional factors. INTRODUCTIONFor close to half a century, the nucleolar channel system (NCS) has been known as an ultrastructural hallmark of the postovulation human endometrium (Dubrauszky and Pohlmann, 1960;Clyman, 1963;Moricard and Moricard, 1964;Terzakis, 1965). It consists of several layers of tubular membrane cisternae in the nuclei of endometrial epithelial cells and is often associated with the nuclear envelope and nucleoli, hence the name (for a review see Spornitz, 1992). This organelle appears during a 3-to 4-d window in the midsecretory phase of the menstrual cycle when the endometrium is receptive to implantation of the fertilized egg (Clyman, 1963;Gordon, 1975). Oral contraceptives and intrauterine devices interfere with the timing and/or overall appearance of the NCS (Wynn, 1967;Feria-Velasco et al., 1972;Azadian-Boulanger et al., 1976;van Santen et al., 1988;Dockery et al., 1997). Several cases of unexplained primary infertility have been characterized by the absence or delayed development of NCSs as the only ultrastructural alteration in endometrial epithelial cells (Kohorn et al., 1972;Gore and Gordon, 1974;Dockery et al., 1996). This and other correlative evidence suggests a role for the NCS in the preparation of the uterine surface for embryo implantation and points to the NCS as a long sought after marker for human receptivity. Nevertheless, despite this tantalizing data and the detailed ultrastructural description of the NCS, it has resisted molecular characterization. Here we provide first insights as to the composition of this enigmatic organelle.On the basis of their remarkable ultrastructural resemblance to the NCS, we previously characterized membrane systems, R-rings, that are induced in nuclei of tissue culture cells by overexpression of the nucleolar protein Nopp140 (Isaac et al., 1998;Isaac et al., 2001). Nopp140 functions as a chaper...

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“…The above data were originally taken as clear evidence of the need to treat asymptomatic endometriosis [12], but the recent evidence of the temporary effect of drug therapy [7] weakens these conclusions. Hence, while there appears to be a need to treat, we are left with the dilemma of 'what drug and for how long'.…”

Section: Resultsmentioning

confidence: 99%

“…Gestrinone (Roussel Uclaf, Paris) is a 19-nosteroid derivative which has androgenic, anti-progcstogenic and anti-oestrogenic actions [7,8]. In controlled studies, it has been shown to be effective in the treatment of endomet riosis [9,10].…”

Section: A Placebo-controlled Trial Of Gestrinone In Asymp Tomatic Enmentioning

confidence: 99%