Action of Cyclic Guanosine Monophosphate on Thyroid Weight and Protein

Pisarev, Mario A.; DeGroot, Leslie J.; Wilber, John F.; Altschuler, N

doi:10.1210/endo-88-4-1074

Cited by 19 publications

(7 citation statements)

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“…The interaction between cyclic AMP and cyclic CMP was studied. Previous work from our laboratory showed that cyclic GMP stimulates the in vivo thyroid protein bio¬ synthesis (Pisarev et al 1971), and the results presented here confirm that both nucleotides have a parallel action on protein and RNA synthesis. This is consistent with the hypothesis on the role of cyclic AMP in the mediation of hormone action (Schell-Frederick 8c Dumont 1970).…”

Section: Resultssupporting

confidence: 87%

“…Furthermore, cyclic CMP also blocked the in vitro stimulatory action of cyclic AMP on the incorporation of [3H]leucine into protein, and of [3H]uridine into RNA. Moreover, cyclic GMP has also been shown to stimulate thyroid protein biosynthesis (Pisarev et al 1971;DeNayer 1973) and guanylate cyclase and protein kinase sensitive to cyclic AMP and cyclic GMP have been found in the thyroid (Barmasch et al 1973;Kuo et al 1970). Thyrotrophin (TSH) regulates thyroid protein biosynthesis mediated by adenylate cyclase and cyclic AMP (Schell-Frederick 8c Dumont 1970).…”

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confidence: 99%

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Action of Cyclic Nucleotides on Protein and Rna Synthesis in the Thyroid

Pisarev

1977

Acta Endocrinologica

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Thyrotrophin (TSH) regulates the biosynthesis of thyroid protein and RNA. This action is mediated by adenylate cyclase and cyclic AMP. In the present study the action of cyclic GMP and cyclic CMP was investigated in beef slices. Both cyclic AMP and cyclic GMP significantly increased the incorporation of [3H]uridine into RNA. These effects were blocked by actinomycin D, suggesting that their action is located at a preor at a transcriptional step. The PCA soluble fraction radioactivity followed in parallel with the variations observed in the RNA fraction, supporting the view that cyclic nucleotides may regulate RNA by modulating the nucleotide precursors pool. * Established investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina.Abbreviations: TSH: thyrotrophin; TCA: trichloroacetic acid: PCA: perchloric acid; cyclic AMP, GMP and CMP stand for the 3'5' cyclic monophosphate compounds of adenosine, guanosine and cytidine, respectively.

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Section: Resultssupporting

confidence: 87%

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confidence: 99%

Action of Cyclic Nucleotides on Protein and Rna Synthesis in the Thyroid

Pisarev

1977

Acta Endocrinologica

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“…Previous evidence has shown, in thyroid cells, the existence of NOS activity and NO-mediated cGMP formation (Esteves et al 1992, Millatt et al 1993, Colin et al 1995. Morever, GC activity has been found in rat thyroid tissue (Barmasch et al 1973) and cGMP can influence different thyroid parameters (Pisarev et al 1971, De Nayer 1973.…”

Section: Figurementioning

confidence: 93%

Role of cyclic 3'5' guanosine monophosphate and nitric oxide in the regulation of iodide uptake in calf thyroid cells

Krawiec²,

Silberschmidt³

et al. 1997

Journal of Endocrinology

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Sodium nitroprusside (SNP) spontaneously produces nitric oxide (NO). In many cell types, this activates the soluble form of the enzyme guanylyl cyclase (GC), resulting in the elevation of cGMP. We herein report the role of NO and cGMP on iodide uptake in primary cultures of calf thyroid cells. Iodide uptake is the limiting step in thyroid hormone biosynthesis and a typical functional parameter. The effect of SNP on this parameter was thus determined. In cells treated with TSH for 72 h, addition of 5 m SNP for the last 2 h caused a significant inhibition on iodide uptake, with no change in cells not treated with TSH. This action was mimicked by an analogue of cGMP, 8Br-cGMP, and blocked by reduced hemoglobin, thus suggesting that it is mediated by the GC-cGMP pathway. SNP also inhibited the stimulation caused by forskolin or analogues of cAMP, indicating that the effect takes place in this pathway, which would be distal to cAMP generation. The accumulation of radioiodine by thyroid cells is a consequence of the balance between influx and efflux. The studies demonstrate that SNP does not affect iodide efflux, thus revealing that it inhibits the influx.

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“…Although no increase of guanyl cyclase by TSH stimulation has been reported (Yamashita and Field, 1972), there are several reports on the stimulation by cGMP of thyroidal protein synthesis in vivo and in vitro, and also on the iodination of thyroglobulin, (Pisarev et al, 1971 ;Varrone et al, 1973). In our experiment, cGMP-PDE activity was not augmented by TSH stimulation.…”

Section: Methodsmentioning

confidence: 99%

Changes in the cyclic nucleotides of rat thyroid, pituitary and plasma caused by methylthiouracil treatment.

Ochi¹,

Hachiya

Kajita

et al. 1978

Endocrinol Japon

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SynopsisChanges in the content of cyclic nucleotides (cAMP and cGMP) and related enzyme activities were observed in the rat thyroid, pituitary and plasma during the prolonged increase of endogenous TSH produced by treatment with methylthiouracil (MTU). Experiments were performed after 4 weeks treatment with MTU.The wet weight and cAMP content per wet weight of the thyroid increased 3 and 1.4 times respectively, but cGMP showed a slight decrease. Pituitary weight increased 1.3 times, but cAMP and cGMP content did not change. The cAMP level in plasma also increased about 1.3 times, but cGMP did not increase.The cAMP-phosphodiesterase activity in the thyroid, pituitary and plasma was increased 1.9, 1.4 and 1.3 times respectively after MTU treatment, while cGMPphosphodiesterase showed no significant change. ATPase activity in the thyroid and pituitary was also increased more than 1.5 times after MTU treatment, while 5'-nucleotidase activity decreased remarkably. These data indicate that the metabolism of the cyclic nucleotide system in the thyroid is stimulated by TSH.

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Action of Cyclic Guanosine Monophosphate on Thyroid Weight and Protein

Cited by 19 publications

References 0 publications

Action of Cyclic Nucleotides on Protein and Rna Synthesis in the Thyroid

Action of Cyclic Nucleotides on Protein and Rna Synthesis in the Thyroid

Role of cyclic 3'5' guanosine monophosphate and nitric oxide in the regulation of iodide uptake in calf thyroid cells

Changes in the cyclic nucleotides of rat thyroid, pituitary and plasma caused by methylthiouracil treatment.

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