Action of euptox A from Ageratina adenophora juice on human cell lines: A top-down study using FTIR spectroscopy and protein profiling

André, Rebeca; Catarro, Joana; Freitas, Dália; Pacheco, Rita; Oliveira, M. Conceição; Serralheiro, Maria Luísa; Falé, Pedro L.

doi:10.1016/j.tiv.2019.03.012

Cited by 17 publications

(9 citation statements)

References 31 publications

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“…Chromatography separation was carried out on a Kinetex 1.7 µm C18 100 Å, LC column 150 × 2.1 mm (Phenomenex, California, USA), at flow rate of 150 µL/min. Mobile phase consisted of 0.1% (v/v) of acid formic in water (A) and 0.1% (v/v) of acid formic in acetonitrile (B), the elution conditions were described in André et al [54]. The column and the sampler were maintained at 35 °C and 10 °C, respectively.…”

Section: Methodsmentioning

confidence: 99%

Bioactivities of Centaurium erythraea (Gentianaceae) Decoctions: Antioxidant Activity, Enzyme Inhibition and Docking Studies

et al. 2019

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Centaurium erythraea is recommended for the treatment of gastrointestinal disorders and to reduce hypercholesterolemia in ethno-medicinal practice. To perform a top-down study that could give some insight into the molecular basis of these bioactivities, decoctions from C. erythraea leaves were prepared and the compounds were identified by liquid chromatography-high resolution tandem mass spectrometry (LC–MS/MS). Secoiridoids glycosides, like gentiopicroside and sweroside, and several xanthones, such as di-hydroxy-dimethoxyxanthone, were identified. Following some of the bioactivities previously ascribed to C. erythraea, we have studied its antioxidant capacity and the ability to inhibit acetylcholinesterase (AChE) and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR). Significant antioxidant activities were observed, following three assays: free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) reduction; lipoperoxidation; and NO radical scavenging capacity. The AChE and HMGR inhibitory activities for the decoction were also measured (56% at 500 μg/mL and 48% at 10 μg/mL, respectively). Molecular docking studies indicated that xanthones are better AChE inhibitors than gentiopicroside, while this compound exhibits a better shape complementarity with the HMGR active site than xanthones. To the extent of our knowledge, this is the first report on AChE and HMGR activities by C. erythraea decoctions, in a top-down analysis, complemented with in silico molecular docking, which aims to understand, at the molecular level, some of the biological effects ascribed to infusions from this plant.

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Section: Methodsmentioning

confidence: 99%

Bioactivities of Centaurium erythraea (Gentianaceae) Decoctions: Antioxidant Activity, Enzyme Inhibition and Docking Studies

et al. 2019

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“…Chromatography separation was carried out on an Intensity Solo 2 1.8 µm C18 100 × 2.1 mm column (Bruker Daltonics, Bremen, Germany). The mobile phase, the elution conditions, and data acquisition by mass spectrometry as described in Reference [22]. The column and the sampler were maintained at 35 • C and 10 • C, respectively.…”

Section: Uhplc-hrms/ms Mass Spectrometrymentioning

confidence: 99%

Melanin: Production from Cheese Bacteria, Chemical Characterization, and Biological Activities

Ferraz

Pacheco

Vaz

et al. 2021

IJERPH

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Pigments are compounds of importance to several industries, for instance, the food industry, where they can be used as additives, color intensifiers, and antioxidants. As the current trend around the world is shifting to the use of eco-friendly commodities, demand for natural dyes is increasing. Melanins are pigments that are produced by several microorganisms. Pseudomonas putida ESACB 191, isolated from goat cheese rind, was described as a brown pigment producer. This strain produces a brown pigment via the synthetic Müeller-Hinton Broth. This brown compound was extracted, purified, analyzed by FTIR and mass spectrometry, and identified as eumelanin. The maximum productivity was 1.57 mg/L/h. The bioactivity of eumelanin was evaluated as the capacity for scavenging free radicals (antioxidant activity), EC50 74.0 ± 0.2 μg/mL, and as an acetylcholinesterase inhibitor, with IC50 575 ± 4 μg/mL. This bacterial eumelanin did not show cytotoxicity towards A375, HeLa Kyoto, HepG2, or Caco2 cell lines. The effect of melanin on cholesterol absorption and drug interaction was evaluated in order to understand the interaction of melanin present in the cheese rind when ingested by consumers. However, it had no effect either on cholesterol absorption through an intestinal simulated barrier formed by the Caco2 cell line or with the drug ezetimibe.

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“…Chromatography separation was carried out on an Intensity Solo 2 1.8 µm C18 100 × 2.1 mm column (Bruker Daltonics, Bremen, Germany). The mobile phase, the elution conditions, and data acquisition by mass spectrometry were done as described in [31]. The column and the sampler were maintained at 35 • C and 10 • C, respectively.…”

Section: Chemical Analysis By Hplc-dad and Lc-hrms/msmentioning

confidence: 99%

Effect of Food Preparations on In Vitro Bioactivities and Chemical Components of Fucus vesiculosus

André

Guedes

Melo

et al. 2020

Foods

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Fucus vesiculosus is a brown macroalgae used in food and generally considered safe to be consumed, according to EU Directive (EC 258/97). The aim of this study is to analyze the effect of food preparation on F.vesiculosus of different origins on what concerns its chemical constituents and final bioactivities. The aqueous extract of the seaweeds were obtained at different temperatures, similar to food preparation and then purified by SPE. The compound identification was carried out by Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS/MS) and algae extracts microstructure were observed by Scanning Electron Microscopy (SEM). The activities were determined by using antioxidant activity, inhibition of acetylcholinesterase (AChE) and 3-hidroxi-3-methyl-glutaril-CoA (HMG-CoA) reductase (HMGR) together with Caco-2 cells line simulating the intestinal barrier. The activity of AChE and the HMGR were inhibited by the extracts giving IC50 values of 15.0 ± 0.1 µg/mL and 4.2 ± 0.1 µg/mL, respectively and 45% of the cholesterol permeation inhibition. The main compounds identified were phlorotannins and peptides derivatives. The mode of preparation significantly influenced the final bioactivities. Moreover, the in vitro results suggest that the preparation of F. vesiculosus as a soup could have hypercholesterolemia lowering effect.

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Action of euptox A from Ageratina adenophora juice on human cell lines: A top-down study using FTIR spectroscopy and protein profiling

Cited by 17 publications

References 31 publications

Bioactivities of Centaurium erythraea (Gentianaceae) Decoctions: Antioxidant Activity, Enzyme Inhibition and Docking Studies

Bioactivities of Centaurium erythraea (Gentianaceae) Decoctions: Antioxidant Activity, Enzyme Inhibition and Docking Studies

Melanin: Production from Cheese Bacteria, Chemical Characterization, and Biological Activities

Effect of Food Preparations on In Vitro Bioactivities and Chemical Components of Fucus vesiculosus

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