Action of gonadotrophic hormones on cholesterol side-chain cleavage and cholesterol ester hydrolase in the ovary of the immature rat

Leaver, H.A.; Boyd, George S.

doi:10.1530/jrf.0.0630101

Cited by 5 publications

(1 citation statement)

References 18 publications

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“…The rate limiting step of all steroidogenetic tis¬ sues is the conversion of cholesterol to pregnenolone (Leaver & Boyd 1981). PGF2a might theoreti¬ cally initiate the functional luteolysis by acting at any level of the control mechanism that regulate the pregnenolone formation.…”

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confidence: 99%

Ovarian production of progesterone and 20α-dihydroprogesterone in vitro following prostaglandin F2α induced luteolysis in the superluteinized rat

Torjesen¹,

Aakvaag²

1984

Acta Endocrinologica

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Superluteinized rats were injected with the prostaglandin F2\g=a\(PGF2\g=a\) analogue cloprostenol to induce luteolysis. The treatment decreased progesterone production of ovarian homogenates from 8.9 \m=+-\0.5 to 4.0 \ m=+-\0.7 nmol/ovary/10 min (mean \ m=+-\sem) within 40 min. tochondrial fractions isolated from control and cloprostenol treated animals produced 4.7 \m=+-\0.4 and 2.8 \ m=+-\0.3 nmol progesterone/ovary/10 min, respectively. Thus, the PGF2\g=a\analogue treatment significantly reduced mitochondrial progesterone production. Addition of the 15 000 \m=x\ g supernatant fraction did not influence the progesterone production rates of the mitochondrial fraction. The basal progesterone secretion from quartered ovaries decreased from 1.50 \m=+-\0.15 to 0.38 \m=+-\0.05

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mentioning

confidence: 99%

Ovarian production of progesterone and 20α-dihydroprogesterone in vitro following prostaglandin F2α induced luteolysis in the superluteinized rat

Torjesen¹,

Aakvaag²

1984

Acta Endocrinologica

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Bioassay of fish gonadotrophin by ovarian mitochondrial cholesterol depletion

Deb

Jamaluddin

Bhattacharya

et al. 1985

General and Comparative Endocrinology

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Studies of the mechanism of action of prostaglandin F2α induced luteolysis in rats

Rajkumar¹,

Ganguli²,

Menon³

et al. 1988

Prostaglandins

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The effects of prostaglandin F2 alpha (PGF2 alpha) administration on the utilization of low density lipoprotein (LDL) and progesterone secretion were examined in dispersed luteal cells from rat ovaries. Immature rats were rendered pseudopregnant with administration of pregnant mare serum gonadotropin and human chorionic gonadotropin. Animals were sacrificed at different times after PGF2 alpha (5 mg/kg) or vehicle administration on day-5 of pseudopregnancy. Administration of PGF2 alpha in vivo decreased human chorionic gonadotropin (hCG) binding to luteal cell membranes in vitro but enhanced binding of LDL. Utilization of labelled cholesterol for steroid synthesis from reconstituted LDL [(3H)-CL-LDL] by dispersed luteal cells was enhanced following PGF2 alpha administration. This suggests that the LDL pathway is not suppressed during prostaglandin induced luteolysis. Progesterone and total progestin secretion in response to N6-2'-0-Dibutyryladenosine 3'5'-cyclic monophosphate (cAMP) was decreased at 2, 4 and 24 hours following PGF2 alpha administration demonstrating a post-cAMP defect in steroidogenesis. Addition of the hydroxylated sterols, 20 or 25-OH cholesterol as substrate stimulated progesterone secretion in vehicle treated rats in a dose dependent fashion with 20-OH cholesterol being more potent. Progesterone secretion in response to stimulation with luteinizing hormone (LH) and cAMP from vehicle treated rats was less than that observed with 20 or 25-OH cholesterol, indicating that endogenous substrate may be a limiting factor in steroid synthesis. The maximal capacity of luteal tissue to produce progestins following PGF2 alpha administration was determined with 20-OH cholesterol as the substrate. The results suggest that the post-cAMP defect at 4 hours following PGF2 alpha administration may be due to failure of the cells to mobilize endogenous cholesterol. However at 24 hours following PGF2 alpha administration the decreased ability of luteal cells to convert cholesterol to pregnenolone may contribute to decreased progesterone synthesis.

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Action of gonadotrophic hormones on cholesterol side-chain cleavage and cholesterol ester hydrolase in the ovary of the immature rat

Cited by 5 publications

References 18 publications

Ovarian production of progesterone and 20α-dihydroprogesterone in vitro following prostaglandin F2α induced luteolysis in the superluteinized rat

Ovarian production of progesterone and 20α-dihydroprogesterone in vitro following prostaglandin F2α induced luteolysis in the superluteinized rat

Bioassay of fish gonadotrophin by ovarian mitochondrial cholesterol depletion

Studies of the mechanism of action of prostaglandin F2α induced luteolysis in rats

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