Action of IGF-I on Expression of the Long Form of the Leptin Receptor (ObRb) in the Prepubertal Period and Throughout the Estrous Cycle in the Mature Pig Ovary

Gregoraszczuk, Ewa Ł.; Ptak, Anna; Wojciechowicz, Tatiana; Nowak, Krzysztof W.

doi:10.1262/jrd.18071

Cited by 19 publications

(16 citation statements)

References 23 publications

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“…Furthermore, leptin receptor mRNA increased as the porcine GC luteinized during 96 h in culture. Moreover, OB-Rb gene expression increased during follicular development and in CL of mature porcine ovaries compared with prepubertal ovaries (Gregoraszczuk et al, 2007). These results suggest that the expression of leptin and of the leptin receptor correlates with the maximal progesterone content.…”

Section: Discussionmentioning

confidence: 70%

Expression of leptin and its receptor genes in the ovarian follicles of cycling and early pregnant pigs

Smolińska

Kamiński

Siawrys

et al. 2013

Animal

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Leptin is a polypeptide hormone produced primarily by adipocytes. It has been implicated in the regulation of satiety and energy homeostasis. Leptin has been suggested to play a role in reproduction based on its involvement in the regulation of the hypothalamic-pituitary-gonadal axis via endocrine, paracrine and/or autocrine pathways. The aim of the present study was to localize the cellular distribution of leptin and the long isoform of leptin receptor (OB-Rb) genes in porcine ovarian antral follicles and to compare the expression levels of leptin and OB-Rb mRNAs in porcine granulosa cells (GC), theca interna (TIC) and theca externa (TEC) cells during the luteal phase of the estrous cycle and in early pregnancy. The expression of leptin and OB-Rb genes was detected in GC, TIC and TEC. Significantly higher levels of leptin gene expression in GC were observed during the mid-and late-luteal phases of the cycle than on days 30 to 32 of pregnancy. On days 14 to 16 of pregnancy, leptin mRNA expression was higher than that on days 14 to 16 of the cycle. The expression of the OB-Rb gene in GC and TEC increased during pregnancy in comparison with the analyzed luteal phases of the cycle. Our results validate the hypothesis that locally produced leptin plays a role in the regulation of porcine reproduction at the ovarian level and exerts a direct effect on porcine follicles. The differences in OB-Rb gene expression in porcine GC and theca cells also suggest that their sensitivity to leptin varies in the ovaries of pregnant and cyclic pigs.

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Section: Discussionmentioning

confidence: 70%

Expression of leptin and its receptor genes in the ovarian follicles of cycling and early pregnant pigs

Smolińska

Kamiński

Siawrys

et al. 2013

Animal

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“…It is a well known fact that resistin, like other adipokines such as leptin (Gregoraszczuk et al 2007, Vázquez et al 2015, adiponectin (Chabrolle et al 2007, Palin et al 2012, chemerin (Reverchon et al 2012) or visfatin (Reverchon et al 2013a) can affect reproduction function. The first indication that resistin was involved in the regulation of the female reproductive system may be the discovery that resistin was expressed in rat ovaries throughout the oestrous cycle and was elevated in animals with induced ovarian cysts (Jones et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Resistin is a survival factor for porcine ovarian follicular cells

Rak¹,

Drwal²,

Wróbel³

et al. 2015

Reproduction

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Previously, we demonstrated the expression of resistin in the porcine ovary, the regulation of its expression and its direct effect on ovarian steroidogenesis. The objective of this study was to examine the effect of resistin on cell proliferation and apoptosis in a co-culture model of porcine granulosa and theca cells. First, we analysed the effect of resistin at 1 and 10 ng/ml alone or in combination with FSHand IGF1 on ovarian cell proliferation with an alamarBlue assay and protein expression of cyclins A and B using western blot. Next, the mRNA and protein expression of selected pro-apoptotic and pro-survival regulators of cell apoptosis, caspase-9, -8 and -3 activity and DNA fragmentation using real time PCR, western blot, fluorescent assay and an ELISA kit, respectively, were analysed after resistin treatment. Furthermore, we determined the effect of resistin on the protein expression of ERK1/2, Stat and Akt kinase. Using specific inhibitors of these kinases, we also checked caspase-3 activity and protein expression. We found that resistin, at both doses, has no effect on cell proliferation. The results showed that resistin decreased pro-apoptotic genes, which was confirmed on protein expression of selected factors. We demonstrate an inhibitory effect of resistin on caspase activity and DNA fragmentation. Finally, resistin stimulated phosphorylation of the ERK1/2, Stat and Akt and kinases inhibitors reversed resistin action on caspase-3 activity and protein expression to control. All of these results showed that resistin has an inhibitory effect on porcine ovarian cell apoptosis by activation of the MAPK/ERK, JAK/Stat and Akt/PI3 kinase signalling pathways. Reproduction (2015) 150 343-355

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“…IGF-I appears to have a stimulatory effect on leptin receptor expression in prepubertal pig follicular cells [30]. On the other hand, leptin does not significantly affect IGF-I secretion either in a dose or a time-dependent manner in rat endometrial stromal cells [31].…”

Section: Discussionmentioning

confidence: 99%

Changes in Nocturnal Leptin and Insulin Concentrations in Prepubertal Low Birth Weight Children after Administration of the IGF-I/IGFBP-3 Complex

Íñiguez¹,

Román²,

Ávila

et al. 2009

Horm Res Paediatr

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Background: There is limited information regarding the effects of IGF-I and/or IGFBP-3 on circulating leptin concentrations. Aim: To determine the effects of IGF-I on leptin and insulin concentrations, we examined leptin and insulin nocturnal profiles before and after the administration of the IGF-I/IGFBP-3 complex (Iplex™) to prepubertal, low birth weight children. Methods: We studied 20 prepubertal children (11 boys and 9 girls), born after a full-term pregnancy with a mean birth weight below 2.8 kg. They were studied at the mean age of 7.3 ± 0.5 years (range 4–11 years). Their mean height was –1.8 ± 0.3 SDS and their mean BMI was 0.1 ± 0.2 SDS at the time of the study. The children were studied on 2 separate occasions, the first under basal conditions, and the second time after s.c. administration of 1 mg/kg of Iplex™ at 21.00 h. Blood samples for determination of leptin and insulin were obtained every 60 min between 23.00 h and 07.00 h, while the children were sleeping. In each patient, we calculated the leptin and insulin mean area under the curve, both under basal conditions and after the administration of the IGF-I/IGFBP-3 complex. Results: Mean nocturnal leptin area under the curve exhibited a significant increase (2.7 ± 2.1 vs. 11.2 ± 2.6 ng/ml h, p < 0.05), whereas that for mean insulin showed a slight decrease, which did not reach statistical significance after administration of the IGF-I/IGFBP-3 complex. Conclusion: These findings indicate that administration of the IGF-I/IGFBP-3 complex increases circulating leptin concentrations in short prepubertal, small for gestational age children, suggesting that IGF-I and/or IGFBP-3 may stimulate leptin secretion by adipose tissue.

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Action of IGF-I on Expression of the Long Form of the Leptin Receptor (ObRb) in the Prepubertal Period and Throughout the Estrous Cycle in the Mature Pig Ovary

Cited by 19 publications

References 23 publications

Expression of leptin and its receptor genes in the ovarian follicles of cycling and early pregnant pigs

Expression of leptin and its receptor genes in the ovarian follicles of cycling and early pregnant pigs

Resistin is a survival factor for porcine ovarian follicular cells

Changes in Nocturnal Leptin and Insulin Concentrations in Prepubertal Low Birth Weight Children after Administration of the IGF-I/IGFBP-3 Complex

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