Action of N-Bromosuccinimide on Ascorbic Acid

Barakat, M. Z.; El-Wahab, Mohamed Fathy Abd; El-Sadr, M. M.

doi:10.1021/ac60100a013

Cited by 76 publications

(17 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The vitamin content was expressed in mg 100 g -1 DM. Determination of vitamin C was performed by certification of NBS (N-bromosuccinimide) according to Barakat et al (1955) with modifications (Miranda et al, 2012). The content of vitamin C was expressed in mg vitamin C 100 g -1 DM.…”

Section: Experimental Analytical Proceduresmentioning

confidence: 99%

Influence of contrasting environments on seed composition of two quinoa genotypes: nutritional and functional properties

Miranda

Vega‐Gálvez

Martínez

et al. 2013

Chilean J. Agric. Res.

View full text Add to dashboard Cite

Quinoa (Chenopodium quinoa Willd.) in Chile represents a source of germplasm with high nutritional value. However, there is little information available related to quinoa seed quality grown under contrasting environments. In this study we evaluated the changes on seed composition of seeds of two lowland/coastal quinoa genotypes grown under arid (Vicuña) and cold-temperate (Temuco) conditions in Chile. Results showed that in the case of 'Regalona Baer' and 'Villarrica' genotypes the arid location (with irrigation) led to a significant increase (P < 0.05) in grain yield (4.2 and 5.1 t ha -1 , respectively), soluble dietary fiber (16.8 ± 0.4 and 28.9 ± 2.1 g kg -1 DM, respectively), vitamin B3 (2.44 ± 0.005 and 2.26 ± 0.04 mg 100 g -1 DM, respectively), saponins (3.22 ± 0.38 mg 100 g -1 DM, 'Regalona Baer'), phenolic compounds (19.2 ± 5.48 and 31.92 ± 1.14 mg gallic acid 100 g -1 DM, respectively) and components of proximate analysis, except protein content. The cold-temperate climate (rainfed) affected positively seed size (2.22 ± 0.17 mm 'Villarrica') and 1000 seed weight (3.08 ± 0.08 and 3.29 ± 0.08 g, respectively), as well as insoluble dietary fiber content (112.3 ± 23.8 g kg -1 DM, 'Regalona Baer'). Furthermore, vitamin C was higher in 'Regalona Baer' genotype at arid locality (31.22 ± 4.2 mg 100 g -1 DM), but much higher content was registered in 'Villarrica' genotype at cold-temperate climate (49.3 ± 5.36 mg 100 g -1 DM). The environment-induced relationship among variables and genotypes was consistent with principal component analysis (PCA). The arid region of Vicuña in Chile represents a potential area for quinoa cultivation for lowland/coastal quinoa genotypes, whose nutritional and functional features were affected positively, due to the much more stressing climatic conditions.

show abstract

Section: Experimental Analytical Proceduresmentioning

confidence: 99%

Influence of contrasting environments on seed composition of two quinoa genotypes: nutritional and functional properties

Miranda

Vega‐Gálvez

Martínez

et al. 2013

Chilean J. Agric. Res.

View full text Add to dashboard Cite

show abstract

“…L-Ascorbic acid, dehydroascorbic acid and D-isoascorbic acid were determined colori- metrically (Bessey et al 1947). Monosaccharides interfered with this assay so a titrimetric method (Barakat et al 1955) was used for ascorbic acid assays when sugars were added. The vitamin C saturation test (Varley, 1967) was performed on the experimental subjects to assess their nutritional status with respect to vitamin C .…”

Section: A T E R I a L S A N D Methodsmentioning

confidence: 99%

Absorption of vitamin C from the human buccal cavity

Sadoogh-Abasian

Evered

1979

Br J Nutr

View full text Add to dashboard Cite

I . Ascorbic acid was absorbed across the mucosa of the human mouth. 2. Omission of sodium ions from the medium decreased the absorption of ascorbic acid. 3. The presence of D-glucose, or 3-O-methyl-~-ghcose, increased the absorption of ascorbic acid but 4. Calcium ions also increased ascorbic acid absorption probably by a secondary effect on Na+ fluxes. 5. Buccal mucosa was also permeable to dehydroascorbic acid and D-isoascorbic. acid D-fructose had little effect and D-mannitol had no effect.Investigations using in vitro techniques have revealed sodium ion-dependent active transport of L-ascorbic acid from the small intestine of the guinea-pig (Stevenson & Brush, 1969) and the human (Stevenson, 1974). Both species have a nutritional requirement for vitamin C.Transport of certain food sugars across human buccal mucosa in vivo shows stereospecificity. For the natural isomers D-glucose and D-galactose the transport is at least partly sodium-dependent (Manning & Evered, I 976).It was the purpose of the present study to investigate the buccal absorption in vivo of L-ascorbic acid and compare it with the results obtained from small intestine. Other workers have already shown that buccal mucosa is permeable to ascorbic acid (Odumosu & Wilson, 1971.L-Ascorbic acid, analytical grade (Fisons Scientific Apparatus Ltd, Loughborough, England), dehydroascorbic acid (INC Pharmaceuticals Inc., Cleveland, Ohio, USA) and D-isoascorbic acid, Na salt (British Drug Houses Ltd, Poole, Dorset), were dried overnight over desiccant under vacuum. Other compounds used were analytical grade when available.Buccal absorption was measured as follows: 25 ml sample of the ascorbic acid solution prepared in appropriate buffer solution was preincubated at 37" for 5 min. Then the solution was circulated inside the mouth by the tongue and cheeks about once a second for 5 min. The solution was expelled into a beaker and the mouth rinsed for 5 sec with 10 ml of preincubated buffer without added ascorbic acid. This was expelled into the same beaker and the volume made up to 50 ml with buffer solution. After mixing a portion was then centrifuged at 3000 g for 10 min to remove insoluble material. At the beginning of each experiment a buccal blank, consisting of 25 ml of buffer solution at 37" but without ascorbic acid, was circulated in the mouth for 5 min then treated as before. Modified Krebs-Ringer buffer at pH 6 in mM concentrations: NaCl, 118.5; KCl, 4.7; KH,PO,, 1.2; MgSO,, 1.2; sodium citrate, 1.8; Na,HPO,, 6.2. For experiments with sodium-free buffer the sodium salts were substituted by potassium salts. Calcium ions were omitted otherwise calcium phosphate was precipitated. For experiments in which calcium was added (2.6 mM) the KH,PO,, Na,HPO, and sodium citrate were omitted and glycylglycine (1.8 mM) added (Evered & Sadoogh-Abasian, 1979). L-Ascorbic acid, dehydroascorbic acid and D-isoascorbic acid were determined colori-

show abstract

“…The amounts of B vitamins (as water soluble B1, B2, B3, B5, B6, B7, B9) in quinoa seed were made according to Kıvrak 2015 in UHPLC-MS / MS device. Vitamin C content was by certification of NBS (N-bromosuccinimide) determined according to Barakat et al (1955). Free amino acid profile analysis in quinoa seed were made using UHPLC-MS / MS device according to Kıvrak (2015) method.…”

Section: Analysesmentioning

confidence: 99%

Investigation of Some Quinoa (Chenopodium Quinoa) Genotypes in Terms of Quality Criteria

Koç

Çetin

2020

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

View full text Add to dashboard Cite

ABSRACT: Quinoa is a rapidly growing plant in the world in the last 20 years. The main reasons for this are that the nutritional contents are important for human health and food benefits. In this study, some quality criterion contents (Moisture, protein, saponin, fat, vitamin C, vitamin B, amino acid, mineral substance) of 20 genotypes belonging to quinoa, which is considered as a new plant for our country, were examined. Protein content is between 12.07% -13.19%, saponin content is between 0.82-1.87%, fat content is between 5.7-6.3%, vitamin C content is between 4.62-10.3 (mg kg -1 ), changed.

show abstract

Action of N-Bromosuccinimide on Ascorbic Acid

Cited by 76 publications

References 29 publications

Influence of contrasting environments on seed composition of two quinoa genotypes: nutritional and functional properties

Influence of contrasting environments on seed composition of two quinoa genotypes: nutritional and functional properties

Absorption of vitamin C from the human buccal cavity

Investigation of Some Quinoa (Chenopodium Quinoa) Genotypes in Terms of Quality Criteria

Contact Info

Product

Resources

About