ACTION OF OESTRADIOL-17β AND CYCLIC AMP ON THE SYNTHESIS OF RNA, PHOSPHOPROTEINS AND PHOSPHOLIPIDS IN THE UTERUS OF OVARIECTOMIZED RATS IN VITRO

Rao, K. N.; Gp, Talwar

doi:10.1677/joe.0.0540215

Cited by 8 publications

(3 citation statements)

References 20 publications

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“…Possibly the best indication of this functional interdependence is provided by studies on the anucleolate mutant of Xenopus laevis, where synthesis of both rRNA and polyamines is virtually absent (Russell, 1971). Our failure to stimulate RNA synthesis with cyclic AMP is at variance with previous reports on other steroid-sensitive systems, such as rat uterus (Sharma & Talwar, 1970;Rao & Talwar, 1972). It should be emphasized, however, that the selection of radioactive precursor (Miller & Baggett, 1972) and large changes in the pools of nucleotides (Oliver & Kellie, 1970;Greenman, 1970Greenman, , 1971 are known to influence dramatically the incorporation of radioactive precursors into uterine RNA during whole-tissue incubations.…”

Section: Discussioncontrasting

confidence: 88%

A reappraisal of the effects of adenosine 3′:5′-cyclic monophosphate on the function and morphology of the rat prostate gland

Ae²,

1973

Biochemical Journal

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1. A comparison was made of the binding of 5alpha-dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one) and cyclic AMP in the rat prostate gland. Distinct binding mechanisms exist for these compounds, and cyclic AMP cannot serve as a competitor for the 5alpha-dihydrotestosterone-binding sites and vice versa. In contrast with the results obtained with 5alpha-dihydrotestosterone, very small amounts of cyclic AMP are retained in nuclear chromatin and the overall binding of this cyclic nucleotide is not markedly affected by castration. 2. Androgenic stimulation does not lead to major increases in the adenylate cyclase activities associated with any subcellular fraction of the prostate gland. Accordingly, changes in the concentration of cyclic AMP in the prostate gland after hormonal treatment are likely to be small, but these were not measured directly. 3. When administered to whole animals in vivo, small amounts of non-degraded cyclic AMP are found in the prostate gland but sufficient to promote an activation of certain carbohydrate-metabolizing enzymes in the cell supernatant fraction. The stimulatory effects of cyclic AMP were not evident with cytoplasmic enzymes engaged in polyamine synthesis or nuclear RNA polymerases. These latter enzymes were stimulated solely by the administration of testosterone. 4. By making use of antiandrogens, a distinction can be drawn between the biochemical responses attributable to the binding of 5alpha-dihydrotestosterone but not of cyclic AMP. Evidence is presented to suggest that the stimulation of RNA polymerase, ornithine decarboxylase and S-adenosyl-l-methionine decarboxylase is a consequence of the selective binding of 5alpha-dihydrotestosterone. Only the stimulation of glucose 6-phosphate dehydrogenase can be attributed to cyclic AMP or other metabolites of testosterone. 5. Overall, this study indicates that the formation of cyclic AMP is not a major feature of the androgenic response and affects only a restricted number of biochemical processes. Certainly, cyclic AMP cannot be considered as interchangeable with testosterone and its metabolites in the control of the function of the prostate gland. This difference is additionally emphasized by the failure of cyclic AMP to restore the morphology of the prostate gland in castrated animals; morphological restoration only follows the administration of androgens.

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Section: Discussioncontrasting

confidence: 88%

A reappraisal of the effects of adenosine 3′:5′-cyclic monophosphate on the function and morphology of the rat prostate gland

Ae²,

1973

Biochemical Journal

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“…Studies were mainly on gamete physiology and regulation of gametogenesis and fertility control measures in terms of promoting or inhibiting fertilization using rodents (Raj and Moudgal, 1970;Gaur and Talwar, 1975;Talwar et al, 1979) and non-human primate models (Moudgal et al, 1971;Anand Kumar et al, 1980. Experimental approaches involved gonadectomy models (Rao and Talwar, 1972;Lohiya and Dixit, 1974), reproductive endocrinology (Mukku and Moudgal, 1970;Singh et al, 1971), in vitro culture of reproductive tissues and their derived cell types (Talwar and Sharma, 1975;Paul et al, 1978) and reproductive toxicological studies (Kar et al, 1967). Besides, work was also carried out on screening of Indian plants for antifertility effect (Satyavati, 1984) and on endocrine disruptors (Batra, 1966).…”

Section: Gamete Development and Functionmentioning

confidence: 99%

Research on early mammalian development in India

Seshagiri¹,

Vani²

2020

Int. J. Dev. Biol.

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Historically, research in India on early mammalian development had only begun, rather modestly, in the last century, unlike the USA and UK. In India, initial studies were on gonadal and reproductive tissue development and function and they were limited to anatomical and histological characterization. This was followed by research on fertility regulation and contraception. Since the 1960s, a major initiative took place regarding endocrine biochemistry and the use of antifertility agents in inhibiting gonadal function and early development. Post-independence, the Indian government´s funding support enabled universities and institutions to embark on various research disciplines in biology but with no particular emphasis on developmental biology per se. Subsequently, India made significant progress in the area of mammalian reproduction and development, but not specifically in the core aspects of developmental biology. Reasons for this could be due to the nation’s compulsion to invest and embark on socio-economic and infrastructure development and on research involving family planning methods for reversible-affordable contraceptives to curtail population growth. With regard to the latter, biologists were involved in hormone-based contraception research. During this pursuit, insights were achieved into basic aspects of the development of gonads, gametes and embryos. Notwithstanding this, in the post-1980s through to the present time, Indian scientists have contributed to (i) the understanding of the cellular and molecular regulation of early development, (ii) developing genetically modified mouse models, (iii) using assisted reproductive technologies, generating mammalian progeny, including humans and (iv) deriving pluripotent stem cell lines for developmental studies. This article provides a perspective on the past and current status of early mammalian development research in India.

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“…injection of diethylstilboestrol into ovariectomized rats. Furthermore, Hechter, Yoshinaga, Halkerston & Birchall (1967) showed that exo¬ genous cyclic AMP mimicked the anabolic effects of oestradiol on the rat uterus in vitro with respect to RNA, protein, lipid and glycogen synthesis (see also Sharma & Talwar, 1970;Rao & Talwar, 1972;Singhal & Lafreniere, 1972) and Griffin & Szego (1968) obtained stimulation of uterine amino acid uptake in vitro by cyclic AMP.…”

Section: Introductionmentioning

confidence: 94%

MECHANISM OF OESTRADIOL ACTION ON THE RAT UTERUS: INDEPENDENCE OF CYCLIC AMP, PROSTAGLANDIN E2 AND Β-Adrenergic MEDIATION

Zor¹,

Koch²,

Lamprecht³

et al. 1973

Journal of Endocrinology

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The hypothesis that cyclic AMP plays an essential role in mediating the biological action of oestradiol on the uterus, was tested by determining the tissue concentration of the cyclic nucleotide after incubation of uteri of immature rats with oestradiol or after injection of this steroid into immature or ovariectomized rats. The effect of known stimulants of uterine adenyl cyclase, namely \g=b\-adrenergicdrugs and prostaglandin E2 (PGE2), on the level of cyclic AMP in the uterus was also examined both in vitro and in vivo.In either system, oestradiol failed to enhance the concentration of cyclic AMP in the uterine tissue, whereas adrenaline or the almost purely \ g = b \ \ x = r e q -\ adrenergic agonist isoprenaline (isoproterenol) caused cyclic AMP accumulation that was susceptible to inhibition by the \g=b\-adrenergicblocking agent propranolol. Prostaglandin E2, and to a much lesser degree prostaglandin F2\ g=a\ , increased cyclic AMP concentration in the uterus, but the effect of PGE2 was not inhibited by propranolol. It may be concluded that oestradiol does not cause appreciable stimulation of PGE2 synthesis or activation of \g=b\-adrenergicreceptors in the rat uterus since, otherwise, increased cyclic AMP production should have been observed after the treatment with oestradiol.Isoprenaline mimicked the stimulatory action of oestradiol on uterine ornithine decarboxylase. However, this action of isoprenaline was abolished by propranolol, whereas that of oestradiol was only slightly, though significantly, inhibited.The present findings do not support the view that the action of oestradiol on the uterus is mediated by cyclic AMP, and also suggest that \g=b\-adrenergic receptors and PGE2 can have only a minor role, if any, in the mechanism of action of this hormone. * In partial fulfilment of the requirements for the Ph.D. decree of the Graduate School of the Weizmann

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ACTION OF OESTRADIOL-17β AND CYCLIC AMP ON THE SYNTHESIS OF RNA, PHOSPHOPROTEINS AND PHOSPHOLIPIDS IN THE UTERUS OF OVARIECTOMIZED RATS IN VITRO

Cited by 8 publications

References 20 publications

A reappraisal of the effects of adenosine 3′:5′-cyclic monophosphate on the function and morphology of the rat prostate gland

A reappraisal of the effects of adenosine 3′:5′-cyclic monophosphate on the function and morphology of the rat prostate gland

Research on early mammalian development in India

MECHANISM OF OESTRADIOL ACTION ON THE RAT UTERUS: INDEPENDENCE OF CYCLIC AMP, PROSTAGLANDIN E2 AND Β-Adrenergic MEDIATION

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