Action of permethrin on Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) semi-engorged females: Morpho-physiological evaluation of salivary glands

Nodari, Elen Fernanda; Roma, Gislaine Cristina; Furquim, Karim Christina Scopinho; Bechara, Gervásio Henrique; Camargo‐Mathias, Maria Izabel

doi:10.1016/j.ttbdis.2012.03.008

Cited by 14 publications

(22 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, the presence of myelin figures and lysosomes (structures that should only be observed in glands in more advanced stages of engorgement) was also observed. These data corroborate Nodari et al [16][17][18], who reported that permethrin would be accelerating the degeneration of the salivary glands of the R. sanguineus s. l. females subjected to different concentrations of the product.…”

Section: Discussionsupporting

confidence: 90%

“…Thus, the ultrastructural and cytochemical data obtained in this study complement studies by Nodari et al [16][17][18], confirming the neurotoxic action [9], of permethrin. Even in lower concentrations than the ones commercially recommended, the product would act on the morphophysiology of the salivary emergent Life Sciences Research Nodari et al glands of R. sanguineus s. l. semi-engorged females, accelerating the degeneration process through atypical cell death, by apoptosis (fragmentation) and autophagy (vacuolation) simultaneously.…”

Section: Discussionsupporting

confidence: 89%

“…The ultrastructural data regarding the salivary glands of the females from the control group corroborate light microscopy analyses by Nodari et al [16][17][18] , who demonstrated that in this group the acini were intact, maintaining original shape; i.e., rounded, and presenting intact cells. The acini I cells presented a well-defined basal membrane labyrinth, as described in literature [22], with randomly distributed mitochondria throughout the cytoplasm and the presence of lipid inclusions (drops).…”

Section: Discussionsupporting

confidence: 84%

“…Considering this, the present study had the objective to cytochemically analyze the alterations in the nucleolar structure and in the ultrastructure of the salivary gland cells of R. sanguineus s. l. semi-engorged females subjected to different concentrations of permethrin (206, 1031 and 2062 ppm). Studies by Nodari et al [16][17][18] have demonstrated that this pyrethroid would accelerate the degeneration of the glandular tissue in this species.…”

Section: Introductionmentioning

confidence: 79%

“…Studies on tick salivary glands degeneration [11][12][13], mainly addressing the influence of synthetic acaricides on the morphophysiology [14][15][16][17][18] and ultrastructure, are scarce in the literature. Considering this, the present study had the objective to cytochemically analyze the alterations in the nucleolar structure and in the ultrastructure of the salivary gland cells of R. sanguineus s. l. semi-engorged females subjected to different concentrations of permethrin (206, 1031 and 2062 ppm).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Morpho-ultrastructural alterations in the salivary glands of semi-engorged Riphicephalus sanguineus s.l. females subjected to permethrin

2017

ELSR

View full text Add to dashboard Cite

Ticks are arthropods of great medical-veterinary importance, and several methods have been developed to control them. The synthetic acaricide permethrin is one of the most widely used substances to control this pest. The present study brings cytochemical and ultrastructural data on the alterations in the salivary gland cells of Rhipicephalus sanguineus sensu lato semi-engorged females subjected to 206, 1031, and 2062 ppm of permethrin, showing that this chemical acts on the glandular acini, affecting the morpho-physiology of their cells, making them irregular, causing intense cytoplasmic vacuolation, altering the mitochondria and causing the emergence of lysosomes, myelin figures and spherocrystals. In addition, the results demonstrated that the chemical affected the nuclei and nucleoli of the cells, causing alterations in size, disorganizing and fragmenting them. Such alterations were mainly observed when processes of apoptotic death occurred, and indicated the occurrence of precocious degeneration of the glandular tissue caused by the exposure to the product, even in lower concentrations than the ones recommended by the manufacturer. Under normal conditions, this process would only occur in the end of the engorgement period. Therefore, these findings confirmed and demonstrated that, even at low concentrations, this acaricide would be able to accelerate the process of glandular tissue degeneration through atypical cell death, where the cell death by apoptosis (fragmentation) and autophagy (vacuolation) occur simultaneously.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Morpho-ultrastructural alterations in the salivary glands of semi-engorged Riphicephalus sanguineus s.l. females subjected to permethrin

2017

ELSR

View full text Add to dashboard Cite

show abstract

The occurrence of autophagic cell death in the tegument of rabbits pre‐infested withRhipicephalus sanguineusand exposed to selamectin (active principle of acaricide pfizer revolution^®)

Bozzatto

Oliveira

Furquim

et al. 2013

Microscopy Res & Technique

Self Cite

View full text Add to dashboard Cite

Ticks of Rhipicephalus sanguineus species have great medical and veterinary importance for being a vector of various diseases. In an attempt to minimize their action on the host, people have resorted to chemical control by using various acaricides, such as selamectin. Although previous studies have demonstrated its toxic action in domestic animals, no studies focused on the detection of cell death when exposed to selamectin. For this reason, the technique for detecting autophagic cell death was used in order to demonstrate the responses of rabbits' skin tissues pre-infested with R. sanguineus and exposed to different concentrations of selamectin. The obtained results when exposed to 100 and 80% concentrations of selamectin showed a strong mark of acid phosphatase on the cells of the connective tissue of the dermis and hair follicles, whereas the ones exposed to the 50% concentration had a weak mark on the cells of the connective tissue of the dermis and moderate staining in hair follicles. It became clear that, when used at high concentrations (100 and 80%), selamectin is capable to induce a large scale occurrence of the autophagic cell death process. On the other hand, the concentration of 50% causes minor morphophysiological changes in the skin of rabbit hosts when evaluated the cell death process. Therefore, the data confirms that selamectin is a powerful dose-dependent toxic agent causes increased activity of the enzyme acid phosphatase.

show abstract

One-month comparative efficacy of three topical ectoparasiticides against adult brown dog ticks (Rhipicephalus sanguineus sensu lato) on mixed-bred dogs in controlled environment

Varloud

Fourie

2015

Parasitol Res

View full text Add to dashboard Cite

This study was designed to compare the therapeutic and residual efficacy for 1 month of three topical ectoparasiticides on mixed-bred dogs against the brown dog tick, Rhipicephalus sanguineus. Adult dogs (n = 32, 10.8–18.4 kg BW) were allocated to 4 groups (n = 8) and infested with 50 adult ticks on days −8, −2, 7, 14, 21, and 28. Within each group, dogs were treated topically on day 0 with a control solution (CS), Vectra® 3D (DPP), Frontline® Plus (FM), or K9 Advantix® (IP). Ticks were enumerated on dogs 24 h after treatment and each subsequent tick infestation by in situ thumb count assessment without removal and at 48 h by combing and removal. Acaricidal efficacy was calculated using arithmetic means for all 24 and 48 h tick count assessments. From 42 to 56 % of the total, infested ticks were found on dogs 48 h post-challenge in the CS group. Therapeutic efficacy for all treatments ranged from 45.5 to 64.6 % after 48 h of infestation. Residual efficacy after FM treatment was consistently lower compared to DPP or IP treatments at the 24 h assessments on days 8, 22, 23, and 29. Residual efficacy measured at this last time point was 94.8 % for DPP, 83.1 % for IP, and 46.9 % for FM. This study demonstrates that permethrin-based formulations (DPP and IP) provided a quicker onset of residual protection against brown dog ticks compared to FM. Although DPP and IP are both permethrin-based formulations, DPP exhibited consistently higher residual acaricidal efficacies and was the only treatment that provided >90 % protection for 1 month at 24 h post challenge.

show abstract

Action of permethrin on Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) semi-engorged females: Morpho-physiological evaluation of salivary glands

Cited by 14 publications

References 38 publications

Morpho-ultrastructural alterations in the salivary glands of semi-engorged Riphicephalus sanguineus s.l. females subjected to permethrin

Morpho-ultrastructural alterations in the salivary glands of semi-engorged Riphicephalus sanguineus s.l. females subjected to permethrin

The occurrence of autophagic cell death in the tegument of rabbits pre‐infested withRhipicephalus sanguineusand exposed to selamectin (active principle of acaricide pfizer revolution^®)

One-month comparative efficacy of three topical ectoparasiticides against adult brown dog ticks (Rhipicephalus sanguineus sensu lato) on mixed-bred dogs in controlled environment

Contact Info

Product

Resources

About

Action of permethrin on Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) semi-engorged females: Morpho-physiological evaluation of salivary glands

Cited by 14 publications

References 38 publications

Morpho-ultrastructural alterations in the salivary glands of semi-engorged Riphicephalus sanguineus s.l. females subjected to permethrin

Morpho-ultrastructural alterations in the salivary glands of semi-engorged Riphicephalus sanguineus s.l. females subjected to permethrin

The occurrence of autophagic cell death in the tegument of rabbits pre‐infested withRhipicephalus sanguineusand exposed to selamectin (active principle of acaricide pfizer revolution®)

One-month comparative efficacy of three topical ectoparasiticides against adult brown dog ticks (Rhipicephalus sanguineus sensu lato) on mixed-bred dogs in controlled environment

Contact Info

Product

Resources

About

The occurrence of autophagic cell death in the tegument of rabbits pre‐infested withRhipicephalus sanguineusand exposed to selamectin (active principle of acaricide pfizer revolution^®)