Action of plasma jets of a low-current spark discharge on microorganisms (Escherichia coli)

Балданов, Б. Б.; Семенов, А. П.; Ranzhurov, Ts. V.; Nikolaev, E. N.; Gomboeva, S. V.

doi:10.1134/s1063784215110043

Cited by 10 publications

(8 citation statements)

References 8 publications

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“…We have developed a nonequilibrium plasma source operating under atmospheric pressure for generating low-temperature (cold) plasma based on plasma jets of a low-current spark discharge [10,14] under the atmospheric pressure (Fig. 1).…”

Section: Methodsmentioning

confidence: 99%

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Cold atmospheric argon plasma jet source and its application for bacterial inactivation

et al. 2019

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In this work, the efficiency of inactivation of a cold argon plasma jet at atmospheric pressure (APPJ) on the basis of a lowcurrent spark discharge into microorganisms with different characteristics of the cell walls was evaluated. Gram-negative bacteria Escherichia coli M17, gram-positive bacteria Bacillus subtilis 534 and Bacillus cereus IP 5832, and the yeast Saccharomyces cerevisiae were seeded on cultured Petri dishes. A plasma jet with an average power of 0.85 W and a flow rate of argon of 6.7 l/min was directed perpendicular to the Petri dishes with agar. The distance to the agar varied from 0.5 to 3 cm, and the treatment time varied from 5 to 300 s. The efficiency of inactivation was assessed by measuring the area of inactivation zones (where there was no growth of microorganisms). It was shown that gram-negative bacteria E. coli M17 is most susceptible to exposure to the plasma jet, and gram-positive bacteria B. cereus IP 5832 and yeast S. cerevisiae are most stable. It is established that an increase in the treatment time of plasma jets of a low-current spark allows effective inactivation of microorganisms over a much larger area.

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Section: Methodsmentioning

confidence: 99%

“…Most plasma jets have a diameter of a few millimeters. On the basis of the low-current spark discharge in argon at atmospheric pressure, it is possible to receive homogeneous enough plasma jets of big diameters which are perspective, for example, in medicine for treatment of vast wounds [14].…”

Section: Introductionmentioning

confidence: 99%

Cold atmospheric argon plasma jet source and its application for bacterial inactivation

et al. 2019

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“…The optical density A of the band with λ=300 is 0.013±0.001 units of optical density. It corresponds to the concentration of [NO 3 -]=(1.86±0.15)·10 -3 mol/L. The measured value of the sample pH was 2.8±0.1.…”

Section: Experiments Resultsmentioning

confidence: 99%

“…In the last decade, medical and biological effects of cold gas-discharge plasma and plasma radiations have actively been studied and devices based on gasdischarge technologies have been created [1][2][3]. These are plasma generators and generators of various types of plasma, which are the sources of active particles.…”

Section: Introductionmentioning

confidence: 99%

Sources of Gas-Discharge Plasma: Effect of the Absorbed Dose and Active Particle Composition on Physicochemical Transformations in Biological Substrates

Пискарев¹,

Astaf’Eva²,

Иванова³

2018

Sovrem Tehnol Med

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The aim of the investigation was to study the effect of the absorbed dose and composition of active particles of various gas-discharge plasma sources on physicochemical changes in biological substrates.Materials and Methods. A generator of flash corona electrical discharge plasma, pulsed sources of plasma radiation Pilimin IR-10, Pilimin IR-1, and Brig, OUFK-01 Solnyshko quartz ultraviolet irradiator with a DKB-9 low-pressure mercury lamp served as the sources of gas-discharge plasma. The radiation dose was measured using a Fricke dosimeter. Optical density of the solutions was registered with the help of SF-102 spectrophotometer. Fluorat-02 Panorama spectrophotometer was used to study fluorescence spectra of tryptophan in the aqueous solution at 10 mg/L concentration after treatment with gas-discharge plasma sources. Concentration of sulfhydryl (-SH) groups before and after irradiation with Pilimin IR-10 and DKB-9 UV lamp was determined for aqueous solutions of albumin and methemoglobin.Results. Emission spectra of the generator of flash corona electrical discharge plasma and spark discharge plasma in the air for Pilimin IR-10 generator have been analyzed. The spectral data show that plasma of the spark electrical discharge is weakly ionized, thermal radiation serves as the main acting factor.A wide peak in the range of ~360 nm region, where there is a peak of nitrous acid NO 2 absorption, is observed in the spectra of water samples exposed to plasma radiation of Pilimin IR-10, Pilimin IR-1, and Brig generators. The peak has a structure connected with the formation of nitrogen compounds of a more complicated nature. Increase of radiation pulse duration results in the increase of optical density at the wavelengths less than 330 nm. 355-360 nm peak height relative to the substrate does not practically change.Under the action of the mercury lamp radiation, hydrogen peroxide and NH 4 + ions are generated in water. The highest absorption dose is noted after the treatment with flash corona electrical discharge plasma, the lowest with radiation of the DKB-9 UV lamp and Brig generator.The concentration of -SH-groups in tryptophan, albumin, and methemoglobin increases when exposed to plasma radiation. The absorbed dose generated by the Pilimin IR-10 generator exceeds 4.5 times the dose generated by the UV lamp, i.e. the effect is caused by a specific reaction mechanism and is not connected directly with the dose.Conclusion. The main role in the alterations in biological substrates exposed to various plasma sources is played by the composition of the active particles generated by the source. These data allow the development of more effective gas-discharge devices for biomedical purposes and can be employed for implementation of innovation plasma technologies in medicine.

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“…It is observed that the inhibition zone diameter extends with increased plasma exposure time. Some authors have reported that the increase of plasma treatment time leads to increasing effectively the inactivation of microorganisms [31].…”

Section: Resultsmentioning

confidence: 99%

Studying the non-thermal plasma jet characteristics and application on bacterial decontamination

Al-rawaf

Fuliful

Khalaf³

et al. 2018

J Theor Appl Phys

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Non-thermal atmospheric-pressure plasma jet represents an excellent approach for the decontamination of bacteria. In this paper, we want to improve and characterize a non-thermal plasma jet to employ it in processes of sterilization. The electrical characteristics was studied to describe the discharge of the plasma jet and the development of plasma plume has been characterized as a function of helium flow rate. Optical emission spectroscopy was employed to detect the active species inside the plasma plume. The inactivation efficiency of non-thermal plasma jet was evaluated against Staphylococcus aureus bacteria by measuring the diameter of inhibition zone and the number of surviving cells. The results presented that the plasma plume temperature was lower than 34 C at a flow rate of 4 slm, which will not cause damage to living tissues. The diameter of inhibition zone is directly extended with increased exposure time. We confirmed that the inactivation mechanism was unaffected by UV irradiation. In addition, we concluded that the major reasons for the inactivation process of bacteria is because of the action of the reactive oxygen and nitrogen species which formed from ambient air, while the charged particles played a minor role in the inactivation process.

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Action of plasma jets of a low-current spark discharge on microorganisms (Escherichia coli)

Cited by 10 publications

References 8 publications

Cold atmospheric argon plasma jet source and its application for bacterial inactivation

Cold atmospheric argon plasma jet source and its application for bacterial inactivation

Sources of Gas-Discharge Plasma: Effect of the Absorbed Dose and Active Particle Composition on Physicochemical Transformations in Biological Substrates

Studying the non-thermal plasma jet characteristics and application on bacterial decontamination

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