Action of prolactin, IFN-γ, TNF-α and LPS on heme oxygenase-1 expression and VEGF release in human monocytes/macrophages

Malaguarnera, Lucia; Imbesi, Rosa; Rosa, Michelino Di; Scuto, Anna; Castrogiovanni, Paola; Messina, Angelo; Sanfilippo, S

doi:10.1016/j.intimp.2005.04.002

Cited by 51 publications

(34 citation statements)

References 32 publications

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“…Human monocyte-derived macrophages (HMMs) were isolated from fresh buffy coat of healthy volunteers as previously described [29]. The buffy coat were diluted with phosphate-buffered saline (PBS) supplemented with 2.5 mM EDTA and layered onto Ficoll-Hypaque gradients (Gibco, Invitrogen, Milan, Italy).…”

Section: Cellsmentioning

confidence: 99%

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Comparison of YKL-39 and CHIT-1 expression during macrophages differentiation and polarization

Rosa¹,

Tibullo²,

Malaguarnera³

et al. 2013

MRI

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The chitinase-like proteins YKL-39 (chitinase 3-like-2) and Chitortriosidase (CHIT-1) are members of the chitinases family. YKL-39 expression has been associated with osteoarthritis, whereas CHIT-1 activity is regarded as a biochemical marker of macrophage activation. So far, the physiological or pathological role of YKL-39 in the inflammation is still poorly understood. We compared YKL-39 and CHIT-1 modulation during monocyte to macrophage transition and polarization. Gene expression analysis was investigated by real-time PCR from mRNA of human monocytes obtained from buffy coat of healthy volunteers, from mRNA of polarized macrophages to classically activated macrophages (or M 1 ), obtained by interferon-γ and lipopolysaccharide exposure, and from mRNA of alternatively activated macrophages (or M 2 ) obtained by interleukin-4 exposure. We demonstrated different variations of YKL-39 and CHIT-1 production during macrophages polarization. CHIT-1 levels gradually increase in the course of the time with a peak of expression between the fifth and the seventh day of culture. In contrast, YKL-39 expression was unaltered in the diverse stage of HMMs differentiation, but increased significantly in M 1 polarized macrophages and reverted to base levels in M 2 polarized macrophages. These findings indicated that the function of YKL-39 is much more restricted and selective than that exerted by CHIT-1.

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Section: Cellsmentioning

confidence: 99%

“…The cells were further purified using CD14 magnetic beads (Miltenyi Biotec) according to the manufacturer's instructions. The HMMs were cultured in on Iscove's medium supplemented with 10% of fetal calf serum (FCS) 2 mM glutamine and 1% of penicillin/streptomycin (Invitrogen, Milan, Italy) [29].…”

Section: Cellsmentioning

confidence: 99%

Comparison of YKL-39 and CHIT-1 expression during macrophages differentiation and polarization

Rosa¹,

Tibullo²,

Malaguarnera³

et al. 2013

MRI

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“…Therefore, the role of PRL on distinct phases of wound healing appears very interesting. In our previous studies 15,16 , it was shown that PRL receptors were present on macrophages, highlighting a role of PRL in the activation of them. There are evidences 17 , which also indicate that human dermal fibroblasts synthesize and release PRL in vitro, suggesting a potential local source of PRL in human skin.…”

Section: Transmission Electron Microscopymentioning

confidence: 85%

“…It is secreted by different cell-types in response to several growth factors 25,26 ; from our recent studies, it seems that VEGF is also produced by macrophages stimulated by PRL 15,16 . PRL-receptor (PRL-R) is widely expressed in several and distinct tissues, and it was identified in human skin too 27,28 .…”

Section: Discussionmentioning

confidence: 99%

“…It could be possible that keratinocytes, expressing PRL-R and proliferating in response to PRL in vitro 27 , are the potential target cells of PRL produced by fibroblasts, and this PRL-production may represent a time signal for keratinocyte proliferation necessary for restoration of epidermis during re-epithelialization, in which intact skin regenerates over the newly formed tissues. Once again macrophages and then PRL are involved; in fact, stimulation of dermal fibroblasts and the resulting keratinocyte proliferation is supported by cytokines 9 , produced also by macrophages activated by PRL 15,16 . In our previous studies we investigated on PRL role in activating macrophages Licensee to evaluate a possible PRL induction in angiogenesis 15,16 .…”

Section: Discussionmentioning

confidence: 99%

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The role of prolactin in the healing process: A preliminary morphological study

Musumeci¹,

Trovato²,

Avola³

et al. 2013

OA Anatomy

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IntroductionThe authors hypothesized a possible use of prolactin as a therapeutic treatment of skin lesions. We investigated the relationship between topic prolactin-treatment of skin lesions and wound healing process in rats. Materials and methodsSkin lesions were produced in both control and experimental rats. PRLtreatment (100 µl of PRL 2 ng/ml in PBS) was performed by dripping on lesions of experimental rats. Control rats were treated with only PBS. After 1 week, fragments of tissue from the bottom of lesions were obtained from three rats of both control and experimental groups. The authors continued the PRL-treatment to other rats until complete healing. In skin specimens, analysis of the ultrastructural features of cells, cellular aggregates and extra-cellular matrix components were made by transmission electron microscopy. In skin specimens, computerized morphometry measurements of different cell types were also made. ResultsResults showed that progression of the healing process was more advanced in treated rats as evidenced by the different percentages of distinct cell types after 1 week of PRLtreatment. Moreover, in treated rats,

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Is there an increased cardiovascular risk in patients with prolactinoma? A challenging question

Yazıcı

Sünbül

Yaşar

et al. 2021

J of Clinical Ultrasound

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Purpose: Epicardial adipose tissue thickness (EATT) is considered to be a surrogate for visceral fat and a novel cardiovascular risk indicator. Hyperprolactinemia has been shown to be associated with increased cardiovascular risk. The aim was to evaluate the association between EATT, carotid intima media thickness (CIMT), and cardiac functions in patients with prolactinoma.Methods: Patients with the diagnosis of prolactinoma were included. The control group consisted of healthy age matched individuals with normal prolactin levels. Prolactin, fasting blood glucose (FBG), insulin, hemoglobin A1c (HbA1c), alanine aminotransferase (ALT), total cholesterol, triglycerides, and high (HDL) and low density lipoprotein (LDL) cholesterol were measured. EATT, CIMT, cardiac systolic, and diastolic functions were determined using echocardiography.Results: We evaluated 67 patients with prolactinoma (aged 40.7 ± 11.9 years, F/M: 51/16) and 57 controls (aged 42.5 ± 7.4 years, F/M: 36/21). Of the 67 patients, 24 had normal prolactin levels. FBG level was higher in prolactinoma patients than in controls. Patients and controls had similar HbA1c, HOMA-IR, ALT, total, HDL, LDL cholesterol, and triglycerides levels, and similar cardiac systolic and diastolic functions. Prolactinoma patients had greater EATT (3.0 ± 0.5 mm vs. 2.6 ± 0.4 mm, p < 0.001) and CIMT (0.57 ± 0.08 mm vs. 0.52 ± 0.04 mm, p = 0.03) than controls. EATT was correlated with body mass index, FBG, HbA1c, and triglyceride levels.Conclusions: EATT and CIMT were greater in patients with prolactinoma, although they had normal cardiac systolic and diastolic functions. K E Y W O R D S cardiovascular risk, carotid intima media thickness, echocardiography, epicardial adipose tissue thickness, prolactinoma 1 | INTRODUCTION Prolactinoma is the most common pituitary adenoma. 1 Prolactinoma comes into attention due to the classical manifestations of increased prolactin levels which are oligomenorrhea, galactorrhea, infertility, loss of libido, and sexual dysfunction. 2 Prolactin, however, has been implicated in some other metabolic actions, which may become evident in prolactinoma patients. Patients with hyperprolactinemia have been shown to have insulin resistance and high levels of atherogenic lipoproteins. 3-5 Insulin resistance observed in hyperprolactinemic patients has been shown to be associated with endothelial dysfunction, and correction of the

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Action of prolactin, IFN-γ, TNF-α and LPS on heme oxygenase-1 expression and VEGF release in human monocytes/macrophages

Cited by 51 publications

References 32 publications

Comparison of YKL-39 and CHIT-1 expression during macrophages differentiation and polarization

Comparison of YKL-39 and CHIT-1 expression during macrophages differentiation and polarization

The role of prolactin in the healing process: A preliminary morphological study

Is there an increased cardiovascular risk in patients with prolactinoma? A challenging question

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