Action Spectroscopy

Lipson, Edward D.

doi:10.1007/978-1-4684-5988-3_24

Cited by 9 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Action spectroscopy is a crucially important method that provides basic information for identification of photoreceptor molecules mediating a certain photochemical or photobiological response: if carefully determined, an action spectrum should match the absorption spectrum of the photoreceptor (Schfifer and Fukshansky 1984, Lipson 1995, Watanabe 1995. On the basis of action spectra determined for the photodispersal response (Diehn 1969a) and photoaccumulation (Checcucci et al 1976), flavin-like chromophores were suggested as candidates for the photoreceptor pigment mediating Euglena photomovements.…”

Section: Introductionmentioning

confidence: 99%

Discovery of signaling effect of UV-B/C light in the extended UV-A/blue-type action spectra for step-down and step-up photophobic responses in the unicellular flagellate algaEuglena gracilis

et al. 1998

View full text Add to dashboard Cite

Summary. Cultures of unicellular algal flagellate Euglena gracilisgrown in different conditions were subjected to action spectroscopy for step-down and step-up photophobic responses, respectively. The spectral region was extended into the UV-B/C as well as in the UV-A and visible regions with the Okazaki Large Spectrograph as the monochromatic light source. The photophobic responses of the cells were measured with an individual-cell assay method with the aid of a computerized video motion analyzer. In the UV-A and visible regions, the shapes of the action spectra were the so-called UV-A/blue type. In the newly studied UV-B/C region, new action peaks were found at 270 nm for the step-down response and at 280 nm for the step-up one. The absorption spectrum of flavin adenine dinucleotide (FAD) appeared to fit the action spectrum for the stepup response, whereas the shape of the step-down action spectrum, which has a UV-A peak (at 370 nm) higher than the blue peak (at 450 rim), appeared to be mimicked by the absorption spectrum of a mixed solution of 6-biopterin and FAD. These observations might also account for the fact that the UV-B/C peak wavelength at 270 nm of the action spectrum for the step-down response is shorter by 10 nm than the action spectrum for the step-up response at 280 nm.

show abstract

Section: Introductionmentioning

confidence: 99%

Discovery of signaling effect of UV-B/C light in the extended UV-A/blue-type action spectra for step-down and step-up photophobic responses in the unicellular flagellate algaEuglena gracilis

et al. 1998

View full text Add to dashboard Cite

show abstract

“…Thus, the characterization of light exposures for non-visual photobiological responses, including melatonin suppression, circadian phase-shifting, and tumor growth, should include radiometric quantification of irradiance and spectral power distribution [76][77] .…”

Section: Circadian Light Measurementmentioning

confidence: 99%