Actions of Huangqi decoction against rat liver fibrosis: a gene expression profiling analysis

Zhang, Gui-biao; Song, Yanan; Chen, Qilong; Dong, Shu; Lu, Yi‐Yu; Su, Ming-yu; Liu, Ping; Su, Shi-Bing

doi:10.1186/s13020-015-0066-5

Cited by 25 publications

(21 citation statements)

References 51 publications

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“…Meaning that administrating with 3.0 g/kg TCM diet represented a stress factor, which was confirmed by the results of liver enzymes assay (Table ). Similar to our findings, a mixture of AM, CP and GU has been reported to be useful for the prevention of liver injury in rat liver model (Wang, Tong, Li, Cao, & Su, ; Zhang et al, , ). Another study reported that a mixture of A. membranaceus and G. glabra could offer hepatoprotective effects and was indicated by lowering ALT level (but not AST levels) in yellow perch ( Perca flavescens ) (Elabd, Wang, Shaheen, Yao, & Abbass, ).…”

Section: Discussionsupporting

confidence: 91%

Effects of dietary supplementation of Astragalus membranaceus , Codonopsis pilosula , and Glycyrrhiza uralensis extract mixture on growth performance, haematological parameters and hepatopancreatic performance in juvenile Pacific white shrimp ( Litopenaeus vannamei )

et al. 2019

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Pacific white shrimp is the major farmed shrimp species in the world. It is known to be very sensitive to the environmental and management changes such as intensification, which is one of the primary necessities to increase shrimp production, but represents a stressful condition, and needs to be managed properly to diminish its negative effects in aquaculture. In this study, juvenile Pacific white shrimp were fed diets supplementing with equal quantities of Astragalus membranaceus, Codonopsis pilosula and Glycyrrhiza uralensis extracts in different concentrations (0, 1.0, 2.0 and 3.0 g/kg) for 8 weeks. We subsequently estimated the effects of supplemented diets on growth performance, haematological parameters, and histological changes of hepatopancreas. Results revealed that the supplemented mixture had no benefits on growth and survival rate, but had favourable effects on haemocyte count, number of granulocytes, and hemocyanin concentration in the haemolymph (p > 0.05). We observed that R cells and B cells were increased in hepatopancreatic tubules of shrimps fed on supplemented diets. Besides, a diet containing 2.0 g/kg of extract mixture maintained the decreased levels of alanine aminotransferase (p < 0.05) and aspartate aminotransferase (p > 0.05). Among the used concentrations, 2.0 g/kg seemed to be the most suitable concentration. K E Y W O R D S Astragalus membranaceus, Codonopsis pilosula, Glycyrrhiza uralensis, histology, immune response, Litopenaeus vannamei How to cite this article: Prathomya P, Hussein HA, Tran NT, et al. Effects of dietary supplementation of Astragalus membranaceus, Codonopsis pilosula, and Glycyrrhiza uralensis extract mixture on growth performance, haematological parameters and hepatopancreatic performance in juvenile Pacific white shrimp (Litopenaeus vannamei). Aquac Res.

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Section: Discussionsupporting

confidence: 91%

Effects of dietary supplementation of Astragalus membranaceus , Codonopsis pilosula , and Glycyrrhiza uralensis extract mixture on growth performance, haematological parameters and hepatopancreatic performance in juvenile Pacific white shrimp ( Litopenaeus vannamei )

et al. 2019

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“…Huangqi decoction (HQD), a classic traditional Chinese medicine (TCM), consists of radix astragali and radix glycyrrhizae and is used for consumptive disease and chronic liver diseases since the Song Dynasty. In our previous preclinical studies, HQD reduced the liver injury caused by dimethylnitrosamine and decreased the liver fibrosis induced by bile duct ligation (Du et al, 2012 ; Liu et al, 2012 ; Li et al, 2015 ; Zhang G. B. et al, 2015 ).…”

Section: Introductionmentioning

confidence: 95%

Huangqi Decoction Alleviates Alpha-Naphthylisothiocyanate Induced Intrahepatic Cholestasis by Reversing Disordered Bile Acid and Glutathione Homeostasis in Mice

et al. 2017

Front. Pharmacol.

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Intrahepatic cholestasis is a serious symptom of liver disorders with limited therapies. In this study, we investigated the efficacy of Huangqi decoction (HQD), a two-herb classic traditional Chinese medicine (TCM), in the treatment of alpha-naphthylisothiocyanate (ANIT)-induced intrahepatic cholestasis in mice. HQD treatment ameliorated impaired hepatic function and tissue damage. A metabolomics study revealed that the endogenous metabolites significantly affected by HQD were related to bile acid (BA) biosynthesis and glutathione metabolism pathways. HQD treatment decreased the intrahepatic accumulation of cytotoxic BAs, normalized serum BA levels, and increased biliary and urinary BA excretion. Additionally, HQD restored the hepatic glutathione content and suppressed reactive oxygen species (ROS) in cholestatic mice. Protein and gene analysis revealed that HQD increased the expression of the hepatic metabolizing enzymes cytochrome P450 (CYP) 2B10 and UDP glucuronosyltransferase family 1 member A1 (UGT1A1), as well as multidrug resistance-associated protein 2 (Mrp2), Mrp3, and Mrp4, which play crucial roles in BA homeostasis. Further, HQD increased the protein expression of glutamate-cysteine ligase, which is involved in the synthesis of glutathione. Importantly, HQD increased the nuclear expression of nuclear factor-E2-related factor-2 (Nrf2). In conclusion, HQD protects against intrahepatic cholestasis by reversing the disordered homeostasis of BAs and glutathione.

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“…It is made up of Radix Astragali (RA) and Radix Glycyrrhizae (RG). HQD can slow the progress of liver fibrosis (Zhang et al, ) by decreasing the levels of inflammatory factors released by Kupffer cells and inhibiting the activation of hepatic stellate cells (HSC), the extracellular matrix (ECM) and collagen synthesis in the liver (Du et al, ; Liu et al, ). Some components of HQD were reported to show anti‐liver fibrosis effects.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantification of multiple components in rat plasma by UPLC‐MS/MS and pharmacokinetic study after oral administration of Huangqi decoction

Zeng

Wang

et al. 2018

Biomedical Chromatography

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A rapid, sensitive and accurate UPLC-MS/MS method was developed for the simultaneous quantification of components of Huangqi decoction (HQD), such as calycosin-7-O-β-d-glucoside, calycosin-glucuronide, liquiritin, formononetin-glucuronide, isoliquiritin, liquiritigenin, ononin, calycosin, isoliquiritigenin, formononetin, glycyrrhizic acid, astragaloside IV, cycloastragenol, and glycyrrhetinic acid, in rat plasma. After plasma samples were extracted by protein precipitation, chromatographic separation was performed with a C column, using a gradient of methanol and 0.05% acetic acid containing 4mm ammonium acetate as the mobile phase. Multiple reaction monitoring scanning was performed to quantify the analytes, and the electrospray ion source polarity was switched between positive and negative modes in a single run of 10 min. Method validation showed that specificity, linearity, accuracy, precision, extraction recovery, matrix effect and stability for 14 components met the requirements for their quantitation in biological samples. The established method was successfully applied to the pharmacokinetic study of multiple components in rats after intragastric administration of HQD. The results clarified the pharmacokinetic characteristics of multiple components found in HQD. This research provides useful information for understanding the relation between the chemical components of HQD and their therapeutic effects.

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Actions of Huangqi decoction against rat liver fibrosis: a gene expression profiling analysis

Cited by 25 publications

References 51 publications

Effects of dietary supplementation of Astragalus membranaceus , Codonopsis pilosula , and Glycyrrhiza uralensis extract mixture on growth performance, haematological parameters and hepatopancreatic performance in juvenile Pacific white shrimp ( Litopenaeus vannamei )

Effects of dietary supplementation of Astragalus membranaceus , Codonopsis pilosula , and Glycyrrhiza uralensis extract mixture on growth performance, haematological parameters and hepatopancreatic performance in juvenile Pacific white shrimp ( Litopenaeus vannamei )

Huangqi Decoction Alleviates Alpha-Naphthylisothiocyanate Induced Intrahepatic Cholestasis by Reversing Disordered Bile Acid and Glutathione Homeostasis in Mice

Simultaneous quantification of multiple components in rat plasma by UPLC‐MS/MS and pharmacokinetic study after oral administration of Huangqi decoction

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