Actions of Prostaglandin F2α and Prolactin on Intercellular Adhesion Molecule-1 Expression and Monocyte/Macrophage Accumulation in the Rat Corpus Luteum1

Olson, Kristen K.; Anderson, L. E.; Wiltbank, Milo C.; Townson, David H.

doi:10.1095/biolreprod64.3.890

Cited by 8 publications

(3 citation statements)

References 71 publications

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“…Several reports have shown instead the involvement of PRL in the apoptosis/structural luteal regression (8,22,32,35,43). However, toward the end of pregnancy, the rat CL loses its capacity to respond to PRL.…”

Section: Discussionmentioning

confidence: 99%

Activity and expression of different members of the caspase family in the rat corpus luteum during pregnancy and postpartum

Peluffo

Stouffer²,

Tesone

2007

American Journal of Physiology-Endocrinology and Metabolism

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Studies were designed to examine the expression and activity of four caspases that contribute to the initial (caspases-2, -8, and -9) and final (caspase-3) events in apoptosis in the rat corpus luteum (CL) during pregnancy (days 7, 17, 19, and 21 of gestation), postpartum (days 1 and 4), and after injection (0, 8, 16, 24, and 36 h) of the physiological luteolysin PGF2alpha. In addition, the temporal relationship of caspase expression/activity relative to steroid production and luteal regression was evaluated. During pregnancy, the activity of all four caspases was significantly greater on day 19, before a decline in CL progesterone (P) and CYP11A1 levels at day 21 of gestation. The levels of the caspase-3 active fragment (p17, measured by Western blot) also increased at days 19 and 21 of pregnancy. Immunohistochemical analyses detected specific staining for the caspases in luteal cells (large and small) as well as in endothelial cells. However, the percentage of apoptotic cells did not increase in the CL until postpartum. Following PGF2alpha injection, there was a significant decrease in CL P by 24 h, although the activity of all four caspases did not increase until 36 h posttreatment. The active p17 fragment of caspase-3 also significantly increased at 36 h post-PGF2alpha. These results suggest that an increase in the activity of caspases-2, -8, -9, and -3 is associated with the early events of natural luteolysis at the end of pregnancy. Also, the exogenous administration of the luteolysin PGF2alpha may regulate members of the caspase family.

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Section: Discussionmentioning

confidence: 99%

Activity and expression of different members of the caspase family in the rat corpus luteum during pregnancy and postpartum

Peluffo

Stouffer²,

Tesone

2007

American Journal of Physiology-Endocrinology and Metabolism

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“…The senescent cells can potentially attract and activate different immune cells subsets (such as NK cells, B cells, T cells, neutrophils, dendritic cells or monocytes/macrophages), which in turn, can be engaged in the immune response and removal of senescent cells [ 104 ]. Interestingly, two research teams reported ICAM1 expression up-regulation in the senescent cells [ 105 , 106 ], which may facilitate communication with immune cells and induce luteal regression, as described by Olson et al in rats [ 107 ]. In our previous studies, ICAM1 mRNA concentration was up-regulated [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Chemerin Impact on Alternative mRNA Transcription in the Porcine Luteal Cells

Makowczenko

Jastrzebski

Paukszto

et al. 2022

Cells

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Chemerin participates in the regulation of processes related to physiological and disorder mechanisms in mammals, including metabolism, obesity, inflammation, and reproduction. In this study, we have investigated chemerin influence on alternative mRNA transcription within the porcine luteal cell transcriptome, such as differential expression of long non-coding RNAs (DELs) and their interactions with differentially expressed genes (DEGs), differences in alternative splicing of transcripts (DASs), and allele-specific expression (ASEs) related to the single nucleotide variants (SNVs) frequency. Luteal cells were collected from gilts during the mid-luteal phase of the oestrous cycle. After in vitro culture of cells un-/treated with chemerin, the total RNA was isolated and sequenced using the high-throughput method. The in silico analyses revealed 24 DELs cis interacting with 6 DEGs and trans-correlated with 300 DEGs, 137 DASs events, and 18 ASEs. The results enabled us to analyse metabolic and signalling pathways in detail, providing new insights into the effects of chemerin on the corpus luteum functions related to inflammatory response, leukocyte infiltration, the occurrence of luteotropic and luteolytic signals (leading to apoptosis and/or necroptosis). Validation of the results using qPCR confirmed the predicted expression changes. Chemerin at physiological concentrations significantly modifies the transcription processes in the porcine luteal cells.

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“…A histerectomia, retirada cirúrgica do útero, em ovelhas e vacas prolongou a vida do CL e a presença do concepto no útero promoveu a manutenção do CL 60 . O bloqueio da síntese de P 4 resulta da interação da PGF2α com seus respectivos receptores localizados nas células luteais esteroidogênicas 57,58,61,62,63,64 . As prostaglandinas são mediadores locais produzidos por vários tecidos que desempenham um importante papel em processos biológicos e patológicos.…”

Section: Síntese De Prostaglandina Pgf2á No Endométriounclassified

Mecanismos endócrinos e moleculares envolvidos na formação do corpo lúteo e na luteólise: revisão de literatura

Bertan

Binelli

Madureira

et al. 2006

Braz. J. Vet. Res. Anim. Sci.

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ResumoO corpo lúteo (CL) é uma estrutura endócrina transitória formada por células luteais esteroidogênicas pequenas (SLC) e grandes (LLC), que associadas aos fibroblastos e a uma ampla rede de capilares constituem uma estrutura especializada na síntese de progesterona (P 4 ). De maneira geral, para a síntese de P 4 nas células luteais esteroidogênicas, o colesterol se liga a receptores específicos na membrana celular e é transportado ao citosol. Posteriormente, o colesterol dirige-se a membrana mitocondrial interna e por ação da enzima P450scc transforma-se em pregnenolona. No retículo endoplasmático liso a pregnenolona é convertida a P 4 pela enzima 3β-hidroxiesteróide deidrogenase (3β-HSD). A proteína de regulação aguda da esteroidogênese (StAR), o receptor benzodiazepínico tipo periférico (PBR) e a endozepina participam no transporte do colesterol para os diferentes compartimentos mitocondriais. Assim, supõe-se que a capacidade de síntese de P 4 no CL esteja relacionada à concentração celular de receptores que captam o colesterol, às enzimas P450scc e 3β-HSD, e às proteínas celulares transportadoras de colesterol. Na espécie bovina, as LLC são responsáveis por mais de 80% da produção deste hormônio no CL. A menor concentração de proteínas transportadoras de colesterol na mitocôndria parecem limitar a síntese de P 4 nas SLC. A P 4 favorece um meio ambiente uterino apropriado para o desenvolvimento do(s) concepto(s), dependendo da espécie. Na maioria das espécies, a ausência da fertilização ou a incapacidade do concepto em sinalizar sua existência no útero estabelecem a luteólise. Tal evento fisiológico caracteriza-se pela regressão funcional e estrutural do(s) corpo(s) lúteo(s). Para o estabelecimento e a manutenção da prenhez torna-se necessário o bloqueio da luteólise por mecanismos que diferem entre as espécies. Em primatas e equídeos esse reconhecimento ocorre pela secreção de gonadotrofinas específicas e em ruminantes pela presença de fatores anti-luteolíticos. Esta revisão tem como objetivo caracterizar os mecanisnos endócrinos e moleculares envolvidos na formação do corpo lúteo e na luteólise. Introdução

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Actions of Prostaglandin F2α and Prolactin on Intercellular Adhesion Molecule-1 Expression and Monocyte/Macrophage Accumulation in the Rat Corpus Luteum1

Cited by 8 publications

References 71 publications

Activity and expression of different members of the caspase family in the rat corpus luteum during pregnancy and postpartum

Activity and expression of different members of the caspase family in the rat corpus luteum during pregnancy and postpartum

Chemerin Impact on Alternative mRNA Transcription in the Porcine Luteal Cells

Mecanismos endócrinos e moleculares envolvidos na formação do corpo lúteo e na luteólise: revisão de literatura

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