Activated CD8+ T cells from aged mice exhibit decreased activation-induced cell death

Hsu, Hui‐Chen; Shi, Jianzhong; Yang, PingAr; Xu, Xin; Dodd, Christopher H.; Matsuki, Yasunori; Zhang, Huang‐Ge; Mountz, John D.

doi:10.1016/s0047-6374(01)00279-2

Cited by 37 publications

(23 citation statements)

References 54 publications

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“…These data are in line with that from Hsu et al [38] demonstrating that, after 96 h of activation, the percentage of Fas + FasL + CD8 + cells is significantly lower in 18-month-old mice than in young mice. Nevertheless they present a diminished Fas expression in response to anti-μ, aged mice B cells are able to up-regulate other activation markers such as CD69, CD80, CD86 and CD25 at the same magnitude as B cells from young mice.…”

Section: Discussionsupporting

confidence: 92%

B cells from aged mice exhibit reduced apoptosis upon B-cell antigen receptor stimulation and differential ability to up-regulate survival signals

Montes

Maletto

Rodríguez

et al. 2005

Clinical and Experimental Immunology

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Section: Discussionsupporting

confidence: 92%

B cells from aged mice exhibit reduced apoptosis upon B-cell antigen receptor stimulation and differential ability to up-regulate survival signals

Montes

Maletto

Rodríguez

et al. 2005

Clinical and Experimental Immunology

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“…Although more virus-specific CD8 T cells are generated during the peak of a primary response in young mice than in aged mice, there was minimal difference in the number of virus-specific memory CD8 T cells between young and aged mice, suggesting decreased apoptosis of virus-specific CD8 T cells in aged mice (13). It has also been reported that activated CD8 T cells from aged mice exhibit decreased activation-induced cell death in vitro (17).…”

Section: Discussionmentioning

confidence: 87%

Cutting Edge: T Cells from Aged Mice Are Resistant to Depletion Early During Virus Infection

Jiang

Anaraki

Blank³

et al. 2003

The Journal of Immunology

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Aging is associated with decreased expansion of T cells upon stimulation. In young mice, infection induces a transient T cell depletion followed by the development of an Ag-specific T cell response that controls the infection. We found that T cells were depleted early after infection with E55 + murine leukemia retrovirus in young, but not aged, mice. Adoptive transfer experiments showed donor T cells of young, but not aged, mice were depleted due to apoptosis in various tissues of young recipients. However, T cells of neither young nor aged donors were depleted in aged recipients. These results indicate that both environmental and intrinsic cellular properties limit depletion of T cells of aged mice and suggest a novel explanation for the decreased T cell response associated with aging.

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“…Except for intracellular staining of IL-12 (20), single-cell suspensions of thymocytes (1 ϫ 10 6 ) were washed once with FACS buffer (5% FCS and 0.1% sodium azide in PBS), and incubated first with unconjugated anti-CD16/ CD32 (Fc Block; BD PharMingen, San Diego, CA) at room temperature for 20 min. Cells were then incubated with a biotin-conjugated Ab at room temperature for 20 min and washed once with FACS buffer.…”

Section: Flow Cytometry Analysismentioning

confidence: 99%

“…Cells (30,000/sample) were analyzed by flow cytometry on a FACScan (BD Biosciences). For intracellular staining of IL-12 (20), single-cell suspensions prepared from the thymus were incubated with a protein transport inhibitor, GolgiStop, according to the manufacturer's protocol (BD PharMingen) at 37°C for 4 h, then stained with Abs for cell surface Ags as described above and incubated with Cytofix/Cytoperm solution (BD PharMingen) in the dark for 20 min before incubation with the PE-IL-12 p40/p70 Ab. The histogram analysis was performed using WinMDI software (email address: trotter@scripps.edu).…”

Section: Flow Cytometry Analysismentioning

confidence: 99%

IL-12 Inhibits Thymic Involution by Enhancing IL-7- and IL-2-Induced Thymocyte Proliferation

Hsu

Stockard³

et al. 2004

The Journal of Immunology

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IL-12 has been reported to affect thymic T cell selection, but the role of IL-12 in thymic involution has not been studied. We found that in vivo, IL-12b knockout (IL-12b−/−) mice exhibited accelerated thymic involution compared with wild-type (WT) B6 mice. This is characterized by an increase in thymocytes with the early development stage phenotype of CD25−CD44+CD4−CD8− in aged IL-12b−/− mice. Histologically, there were accelerated degeneration of thymic extracellular matrix and blood vessels, a significantly decreased thymic cortex/medulla ratio, and increased apoptotic cells in aged IL-12b−/− mice compared with WT mice. There was, however, no apparent defect in thymic structure and thymocyte development in young IL-12−/− mice. These results suggest the importance of IL-12 in maintaining thymic integrity and function during the aging process. Surprisingly, in WT B6 mice, there was no age-related decrease in the levels of IL-12 produced from thymic dendritic cells. Stimulation of thymocytes with IL-12 alone also did not enhance the thymocyte proliferative response in vitro. IL-12, however, provided a strong synergistic effect to augment the IL-7 or IL-2 induced thymocyte proliferative response, especially in aged WT and IL-12b−/− mice. Our data strongly support the role of IL-12 as an enhancement cytokine, which acts through its interactions with other cytokines to maintain thymic T cell function and development during aging.

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Activated CD8+ T cells from aged mice exhibit decreased activation-induced cell death

Cited by 37 publications

References 54 publications

B cells from aged mice exhibit reduced apoptosis upon B-cell antigen receptor stimulation and differential ability to up-regulate survival signals

B cells from aged mice exhibit reduced apoptosis upon B-cell antigen receptor stimulation and differential ability to up-regulate survival signals

Cutting Edge: T Cells from Aged Mice Are Resistant to Depletion Early During Virus Infection

IL-12 Inhibits Thymic Involution by Enhancing IL-7- and IL-2-Induced Thymocyte Proliferation

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