Activated intestinal microbiome-associated tryptophan metabolism upregulates aryl hydrocarbon receptor to promote osteoarthritis in a rat model

Chen, Liyile; Huang, Zeyu; Li, Qianhao; Chen, Changjun; Luo, Yuling; Kang, Pengde

doi:10.1016/j.intimp.2023.110020

Cited by 6 publications

(12 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of the 38 studies identified in this review, 24 were preclinical in vivo studies ( Table 2 ). Of these, 11 used an OA rat animal model (5 chemically induced OA [ 31 , 32 , 37 , 41 , 43 ], 1 surgically induced OA [ 36 ], 3 high fat diet-induced OA [ 34 , 42 , 44 ], 1 deoxycorticosterone acetate (DOCA) induced [ 46 ] and 1 antibiotic + tryptophan rich diet induced [ 40 ]), 11 used an OA mouse model (7 surgically induced OA [ 26 , 27 , 33 , 35 , 39 , 50 , 51 ], 2 high fat diet induced [ 44 , 45 ], 2 load induced [ 39 , 49 ], 1 used a spontaneous OA model in guinea pigs [ 52 ]) and 1 used a spontaneous OA model in rhesus macaques [ 47 ]. The number of animals used in these studies was variable (range: from 20 to 88) and not reported in a few articles ( n = 3) [ 26 , 31 , 38 ].…”

Section: Resultsmentioning

confidence: 99%

“…The number of animals used in these studies was variable (range: from 20 to 88) and not reported in a few articles ( n = 3) [ 26 , 31 , 38 ]. The main technique used to assess OA was histology, which in some studies was also combined with specific semi-quantitative scores (Mankin’s and Osteoarthritis Research Society International (OARSI)) [ 26 , 33 , 37 , 39 , 40 , 50 ] and/or immunohistochemistry [ 33 , 46 ], ramp test [ 32 ], micro-computed tomography [ 27 , 32 , 46 , 49 , 51 ], anterior drawer test, Magnetic Resonance Imaging [ 47 ], and dual-energy X-ray absorptiometry [ 27 ]. Some articles ( n = 5) were designed to test the changes in the gut microbiota and metabolism during the administration of various supplements, such as quercetin, bioactive compounds from chicken cartilage, extracellular vesicles (EVs) from raw milk, oligofructose and prebiotic fiber, for OA [ 26 , 31 , 32 , 33 , 34 ].…”

Section: Resultsmentioning

confidence: 99%

“…paracasei M5 and Costridium Sulfate) [ 38 ]. Three other studies investigated the association between gut dysbiosis induced by antibiotic administration (ampicillin, neomycin or antibiotic combined with a tryptophan-rich diet) and OA [ 27 , 39 , 40 ]. The effect of traditional Chinese medicine therapies, such as moxibustion and eletroacupuncture (EA), on OA and gut microbiota was reviewed in three articles [ 41 , 42 , 43 ].…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

The Complex Interplay between the Gut Microbiome and Osteoarthritis: A Systematic Review on Potential Correlations and Therapeutic Approaches

Marchese,

Contartese,

Giavaresi

et al. 2023

IJMS

View full text Add to dashboard Cite

The objective of this review is to systematically analyze the potential correlation between gut microbiota and osteoarthritis (OA) as well as to evaluate the feasibility of microbiota-targeted therapies for treating OA. Studies conducted from October 2013 to October 2023 were identified via a search on electronic databases such as PubMed, Web of Science, and Scopus, following established PRISMA statement standards. Two reviewers independently screened, assessed, and extracted relevant data, and then they graded the studies using the ROBINS I tool for non-randomized interventions studies and SYRCLE’s risk-of-bias tool for animal studies. A search through 370 studies yielded 38 studies (24 preclinical and 14 clinical) that were included. In vivo research has predominantly concentrated on modifying the gut microbiota microenvironment, using dietary supplements, probiotics, and prebiotics to modify the OA status. Lactobacilli are the most thoroughly examined with Lactobacillus acidophilus found to effectively reduce cartilage damage, inflammatory factors, and pain. Additionally, Lactobacillus M5 inhibits the development of OA by preventing high-fat diet (HFD)-induced obesity and protecting cartilage from damage. Although there are limited clinical studies, certain compositions of intestinal microbiota may be associated with onset and progression of OA, while others are linked to pain reduction in OA patients. Based on preclinical studies, there is evidence to suggest that the gut microbiota could play a significant role in the development and progression of OA. However, due to the scarcity of clinical studies, the exact mechanism linking the gut microbiota and OA remains unclear. Further research is necessary to evaluate specific gut microbiota compositions, potential pathogens, and their corresponding signaling pathways that contribute to the onset and progression of OA. This will help to validate the potential of targeting gut microbiota for treating OA patients.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

The Complex Interplay between the Gut Microbiome and Osteoarthritis: A Systematic Review on Potential Correlations and Therapeutic Approaches

Marchese,

Contartese,

Giavaresi

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…There is a relationship between their abnormal metabolism and the development and progression of OA [68,69]. First, abnormalities in the tryptophan metabolic pathway may lead to a shift of tryptophan to the synthetic pathway of inflammatory mediators, while reducing the utilization of tryptophan to the synthetic pathway in the growth and repair process, which may lead to damage of the osteoarticular cartilage [70,71]. In addition, tryptophan can be metabolized into serotonin, which has important effects on the growth and differentiation of articular cartilage cells [72], and a deficiency of serotonin may lead to articular cartilage damage [73,74].…”

Section: Discussionmentioning

confidence: 99%

Differential Metabolites in Osteoarthritis: A Systematic Review and Meta-Analysis

Liao,

Han,

Wang

et al. 2023

Nutrients

View full text Add to dashboard Cite

(1) Many studies have attempted to utilize metabolomic approaches to explore potential biomarkers for the early detection of osteoarthritis (OA), but consistent and high-level evidence is still lacking. In this study, we performed a systematic review and meta-analysis of differential small molecule metabolites between OA patients and healthy individuals to screen promising candidates from a large number of samples with the aim of informing future prospective studies. (2) Methods: We searched the EMBASE, the Cochrane Library, PubMed, Web of Science, Wan Fang Data, VIP Date, and CNKI up to 11 August 2022, and selected relevant records based on inclusion criteria. The risk of bias was assessed using the Newcastle–Ottawa quality assessment scale. We performed qualitative synthesis by counting the frequencies of changing directions and conducted meta-analyses using the random effects model and the fixed-effects model to calculate the mean difference and 95% confidence interval. (3) Results: A total of 3798 records were identified and 13 studies with 495 participants were included. In the 13 studies, 132 kinds of small molecule differential metabolites were extracted, 58 increased, 57 decreased and 17 had direction conflicts. Among them, 37 metabolites appeared more than twice. The results of meta-analyses among four studies showed that three metabolites increased, and eight metabolites decreased compared to healthy controls (HC). (4) Conclusions: The main differential metabolites between OA and healthy subjects were amino acids (AAs) and their derivatives, including tryptophan, lysine, leucine, proline, phenylalanine, glutamine, dimethylglycine, citrulline, asparagine, acetylcarnitine and creatinine (muscle metabolic products), which could be potential biomarkers for predicting OA.

show abstract

“…Trp not only decreased the expression of pro-inflammatory factors Lj-TNF-α, Lj-IL-1β, and Lj-NF-κB, but also up-regulated the expression of anti-inflammatory factor Lj-TGF-β. A rat OA model was established using antibiotics models (without antibiotics as control) in combination with a high Trp diet to investigate the pathogenesis of OA by examining the aromatic receptor AhR family 1 cytochrome P450, polypeptide 1 (CyP1A1), bone and cartilage metabolic markers, inflammation, and gut microbial-associated Trp metabolites . The results showed that the severity of cartilaginous OA was positively correlated with the expression of AhR and CyP1A1 in chondrocytes, in which antibiotics reduced the relative abundance of cartilaginous lactic acid bacteria to alleviate synovial inflammation, and the high levels of Trp metabolism antagonized the effectiveness of antibiotics to exacerbate OA.…”

Section: Effects Of Trp Metabolites On Variety Of Diseasesmentioning

confidence: 99%

Recent Advances in Current Uptake Situation, Metabolic and Nutritional Characteristics, Health, and Safety of Dietary Tryptophan

Zhu,

Yang,

Zhao

2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

Tryptophan (Trp) is an essential amino acid which is unable to be synthesized in the body. Main sources of Trp are uptake of foods such as oats and bananas. In this review, we describe the status of current dietary consumption, metabolic pathways and nutritional characteristics of Trp, as well as its ingestion and downstream metabolites for maintaining body health and safety. This review also summarizes the recent advances in Trp metabolism, particularly the 5-HT, KYN, and AhR activation pathways, revealing that its endogenous host metabolites are not only differentially affected in the body but also are closely linked to health. More attention should be paid to targeting its specific metabolic pathways and utilizing food molecules and probiotics for manipulating Trp metabolism. However, the complexity of microbiota−host interactions requires further exploration to precisely refine targets for innovating the gut microbiota-targeted diagnostic approaches and informing subsequent studies and targeted treatments of diseases.

show abstract

Activated intestinal microbiome-associated tryptophan metabolism upregulates aryl hydrocarbon receptor to promote osteoarthritis in a rat model

Cited by 6 publications

References 43 publications

The Complex Interplay between the Gut Microbiome and Osteoarthritis: A Systematic Review on Potential Correlations and Therapeutic Approaches

The Complex Interplay between the Gut Microbiome and Osteoarthritis: A Systematic Review on Potential Correlations and Therapeutic Approaches

Differential Metabolites in Osteoarthritis: A Systematic Review and Meta-Analysis

Recent Advances in Current Uptake Situation, Metabolic and Nutritional Characteristics, Health, and Safety of Dietary Tryptophan

Contact Info

Product

Resources

About