“…the CO 2 evolved from the substrate added with the test material minus the CO 2 evolved from the substrate (blank). As mentioned before, however, the CO 2 is not the only product and, in order to completely describe the biodegradation of a material, it is very important to identify and quantify the possible by-products (Bellia et al, 1999). In fact, the accumulation of slowly or non degradable by-products or residues in soil would mean an overall biodegradability lower than expected and, on the other hand, possible negative effects depending on oxygen uptake for biodegradation, on the possible toxicity of by-products, on the development of competitive biomass, etc.…”