Activated β-catenin induces myogenesis and inhibits adipogenesis in BM-derived mesenchymal stromal cells

Shang, Yan Chang; Zhang, Chao; Wang, S H; Xiong, Fu; Zhao, Chengliang; Peng, Funing; Feng, Shan Wei; Yu, Mei; Li, Min-Rui; Zhang, Y N

doi:10.1080/14653240701508437

Cited by 58 publications

(54 citation statements)

References 46 publications

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“…www.nature.com/aps Shang YC et al Acta Pharmacologica Sinica npg we have found that the ectopic expression of activated betacatenin and pretreatment with Wnt3a-conditioned medium induces bone marrow MSCs to differentiate into myogenic cells [18,19] . These studies collectively demonstrate that Wnt signaling induces myogenesis in adult stem cells.…”

Section: Wwwchinapharcom Shang Yc Et Almentioning

confidence: 93%

“…We have previously demonstrated that the activation of Wnt3a signaling promotes myogenic differentiation of bone marrow MSCs in vitro [18,19] . In the present study, we provide evidence for effective stem cell transplantation therapy by activation of Wnt3a signaling in dystrophic mice.…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies have shown that Wnt3a induces myogenic differentiation in bone marrow MSCs via the ectopic expression of activated beta-catenin or pretreatment with Wnt3a conditioned medium in vitro [18,19] . In the current study, we hypothesized that Wnt3a would improve the myogenic differentiation efficiency of transplanted MSCs and repair the muscle damage in vivo.…”

Section: Acta Pharmacologica Sinica Npgmentioning

confidence: 99%

“…MSCs were induced to differentiate into myogenic, osteogenic, and adipogenic as described previously [18,19] . The percentage of MHC-positive cells was calculated as the ratio of positive cells to the total cells counted.…”

Section: Multilineage Differentiation Of Wnt3a-treated Mscsmentioning

confidence: 99%

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Activation of Wnt3a signaling promotes myogenic differentiation of mesenchymal stem cells in mdx mice

et al. 2016

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Aim: Duchenne muscular dystrophy (DMD) is an X-linked genetic muscular disorder with no effective treatment at present. Mesenchymal stem cell (MSC) transplantation has been used to treat DMD, but the efficiency is low. Our previous studies show that activation of Wnt3a signaling promotes myogenic differentiation of MSCs in vitro. Here we report an effective MSC transplantation therapy in mdx mice by activation of Wnt3a signaling. Methods: MSCs were isolated from mouse bone marrow, and pretreated with Wnt3a-conditioned medium (Wnt3a-CM), then transplanted into mdx mice. The recipient mice were euthanized at 4, 8, 12, 16 weeks after the transplantation, and muscle pathological changes were examined. The expression of dystrophin in muscle was detected using immunofluorescence staining, RT-PCR and Western blotting. Results: Sixteen weeks later, transplantation of Wnt3a-pretreated MSCs in mdx mice improved the characteristics of dystrophic muscles evidenced by significant reductions in centrally nucleated myofibers, the variability range of cross-sectional area (CSA) and the connective tissue area of myofibers. Furthermore, transplantation of Wnt3a-pretreated MSCs in mdx mice gradually and markedly increased the expression of dystrophin in muscle, and improved the efficiency of myogenic differentiation. Conclusion: Transplantation of Wnt3a-pretreated MSCs in mdx mice results in long-term amelioration of the dystrophic phenotype and restores dystrophin expression in muscle. The results suggest that Wnt3a may be a promising candidate for the treatment of DMD.

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Section: Wwwchinapharcom Shang Yc Et Almentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Acta Pharmacologica Sinica Npgmentioning

confidence: 99%

Section: Multilineage Differentiation Of Wnt3a-treated Mscsmentioning

confidence: 99%

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Activation of Wnt3a signaling promotes myogenic differentiation of mesenchymal stem cells in mdx mice

et al. 2016

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“…Pax3 is necessary for skeletal myogenesis and acts upstream of MyoD during skeletal muscle development; Gli factors could induce the expression of Myf-5, which is a direct target of Wnt/β-catenin (Borello et al 2006;Gustafsson et al 2002;Ridgeway and Skerjanc 2001). The Wnt signaling pathway could enhance myogenesis and inhibit adipogenesis in cultured mesenchymal stem cells (Du et al 2011;Shang et al 2007). Livestock carcasses are composed of muscle, fat, connective tissue, and bone; muscle fibers form lean meat, whereas fat cells and fibroblasts are embedded in connective tissue (Du et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Sequencing and characterization of lncRNAs in the breast muscle of Gushi and Arbor Acres chickens

Ren

et al. 2018

Genome

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Chicken muscle quality is one of the most important factors determining the economic value of poultry, and muscle development and growth are affected by genetics, environment, and nutrition. However, little is known about the molecular regulatory mechanisms of long non-coding RNAs (lncRNAs) in chicken skeletal muscle development. Our study aimed to better understand muscle development in chickens and thereby improve meat quality. In this study, Ribo-Zero RNA-Seq was used to investigate differences in the expression profiles of muscle development related genes and associated pathways between Gushi (GS) and Arbor Acres (AA) chickens. We identified two muscle tissue specific expression lncRNAs. In addition, the target genes of these lncRNAs were significantly enriched in certain biological processes and molecular functions, as demonstrated by Gene Ontology (GO) analysis, and these target genes participate in five signaling pathway, as revealed by an analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Taken together, these data suggest that different lncRNAs might be involved in regulating chicken muscle development and growth and provide new insight into the molecular mechanisms of lncRNAs.

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Stem Cells and Their Application in Orthopedics

Li¹,

Zhu²,

Sun³

et al. 2022

Biofabrication for Orthopedics

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Activated β-catenin induces myogenesis and inhibits adipogenesis in BM-derived mesenchymal stromal cells

Cited by 58 publications

References 46 publications

Activation of Wnt3a signaling promotes myogenic differentiation of mesenchymal stem cells in mdx mice

Activation of Wnt3a signaling promotes myogenic differentiation of mesenchymal stem cells in mdx mice

Sequencing and characterization of lncRNAs in the breast muscle of Gushi and Arbor Acres chickens

Stem Cells and Their Application in Orthopedics

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