Activating effect of Tanacetum vulgare L. pectin polysaccharide on ionic channels of neuronal membrane

Abstract

The membranotropic effects of TVF tanacetan pectin polysaccharide derived from Tanacetum vulgare L. was studied by the voltage-clamp method on isolated neurons of Lymnaea stagnalis mollusk. TVF in concentrations of 0.1-10.0 microg/ml nonselectively activated the outward potassium and total inward (sodium and calcium) ionic currents (slightly dose-dependently and reversibly increased their amplitude by 5-10%) and decreased nonspecific leakage current.

Stabilizing effect of Silene pectin polysaccharide on electrical activity of the sinoatrial area in frog heart

“…The observed phenomenon of normal current passage between sinoatrial cells can be attributed to the presence of negatively charged carboxyl groups of galacturonic acid residues in silenan, which facilitates absorption the pectin (at least partial) on the endothelium or basal membrane and increases electrical resistance of tight junctions. This stabilizes the polarized lipid backbone of the sarcolemma containing dynamic protein molecules of ionic channels and ionic pumps and reduces leakage currents [3]. Presumably, due to the long molecule the pectin acts extracellularly.…”

“…The pectin has virtually no toxic effect on mouse macrophages upon intraperitoneal injection. Tanacetum pectin structurally similar to silenan slightly and reversibly increases slow outward K + current in pond snail neurons [3]. However, the mechanisms of pectin effects on stimulated cells remain unclear.…”

“…Silenan, a Silene pectin, is a branched pectin polysaccharide [3]. The pectin has virtually no toxic effect on mouse macrophages upon intraperitoneal injection.…”