Activation and thermal stabilization of a recombinant γ-glutamyltranspeptidase from Bacillus licheniformis ATCC 27811 by monovalent cations

Lin, Long-Liu; Lu, Bo-Yuan; Chi, Meng-Chun; Huang, Yingwei; Lin, Min-Guan; Wang, Tzu-Fan

doi:10.1007/s00253-022-11836-y

Cited by 7 publications

(6 citation statements)

References 90 publications

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“…Theanine is an FDA approved health drink additive. The addition of monovalent cations (Na + , Cs + ) in the presence of Mg 2+ to γ-glutamyl transpeptidase (GGT) from Bacillus licheniformis enhanced the production of theanine by 15% relative to the absence of metal additives [19]. The productivity analysis was carried out in a reaction mixture containing 25 µg mL −1 GGT, 250 mM L-Gln as donor, 600 mM ethylamine as acceptor, ±200 mM Na + or ±Cs + as well as ±200 mM Mg 2+ in 50 mM borate buffer (pH 10.5) at 37 • C for 4 h. The reason for the higher productivity in the presence of monovalent cations was found to be an increase in both the V max (from 17 to 28 µM min −1 ) and thermostability (t 1/2 at 60 • C, from 16 to 74 min −1 ).…”

Section: Higher Productivity Due To Additivesmentioning

confidence: 99%

“…However, the immobilized enzyme could be reused 5 times with only 10% loss in activity up to 3 cycles, thereby minimizing manufacturing costs [28]. Recently, GGT from the same organism showed 15% enhancement in theanine productivity in the presence of metal ion additives (Na + or Cs + + Mg 2+ ) due to the simultaneous increase in activity and stability (see Section 5.3) [19]. It would be interesting to investigate the productivity of theanine with immobilized GGT in the presence of metal ion additives by combining the advantages of immobilization (recycling of GGT) and additives (enhanced productivity).…”

Section: Ggt Catalyzed Processes For the Synthesis Of Pharmaceuticalsmentioning

confidence: 99%

“…This shows the significance of optimization of temperature for maximizing the yield. Recently, the addition of metal ion additives increased the t 1/2 of thermal inactivation of GGT by 5-fold, resulting in increased productivity due to the ability of the enzyme to better resist the thermal unfolding over an extended period (see Section 5.3) [19]. [18] with permission from Elsevier).…”

Section: Lipase Mediated Hydrolysismentioning

confidence: 99%

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Evaluating Enzymatic Productivity—The Missing Link to Enzyme Utility

Siddiqui

Ertan

Poljak

et al. 2022

IJMS

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Kinetic productivity analysis is critical to the characterization of enzyme catalytic performance and capacity. However, productivity analysis has been largely overlooked in the published literature. Less than 0.01% of studies which report on enzyme characterization present productivity analysis, despite the fact that this is the only measurement method that provides a reliable indicator of potential commercial utility. Here, we argue that reporting productivity data involving native, modified, and immobilized enzymes under different reaction conditions will be of immense value in optimizing enzymatic processes, with a view to accelerating biotechnological applications. With the use of examples from wide-ranging studies, we demonstrate that productivity is a measure of critical importance to the translational and commercial use of enzymes and processes that employ them. We conclude the review by suggesting steps to maximize the productivity of enzyme catalyzed reactions.

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Section: Higher Productivity Due To Additivesmentioning

confidence: 99%

Section: Ggt Catalyzed Processes For the Synthesis Of Pharmaceuticalsmentioning

confidence: 99%

Section: Lipase Mediated Hydrolysismentioning

confidence: 99%

See 1 more Smart Citation

Evaluating Enzymatic Productivity—The Missing Link to Enzyme Utility

Siddiqui

Ertan

Poljak

et al. 2022

IJMS

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“…Thus, increasing the spore quantity in the broth is a critical factor in improving the formulation's quality. However, the current research on B. licheniformis primarily revolves around its application effects and mechanisms of action 7–9 . There is a relatively limited amount of research dedicated to studying the promotion of spore formation in B. licheniformis and other spore‐forming bacteria, with a major focus on optimizing the initial culture media and fixed cultivation conditions.…”

Section: Introductionmentioning

confidence: 99%

“…However, the current research on B. licheniformis primarily revolves around its application effects and mechanisms of action. [7][8][9] There is a relatively limited amount of research dedicated to studying the promotion of spore formation in B. licheniformis and other spore-forming bacteria, with a major focus on optimizing the initial culture media and fixed cultivation conditions. In their study, Posada-Uribe successfully enhanced the spore ratio of B. subtilis EA-CB0575 by 94.2% through the optimization of the culture medium and the precise control of agitation speed and aeration rate.…”

mentioning

confidence: 99%

Enhancing Bacillus licheniformis sporulation with automatic nitrogen feeding based on online detection of exhaust CO₂

Ding,

Wang,

Jiang

et al. 2023

AIChE Journal

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The Bacillus licheniformis formulation is effective in environmental remediation, gut microbiota modulation, and soil improvement. Adequate spore quantity is crucial for activity of B. licheniformis formulations. This study aimed to enhance B. licheniformis BF‐002 sporulation by optimizing nitrogen source feeding. In the initial four manual feeding strategies, continuous feeding based on exhaust CO2 resulted in the highest cell and spore quantities (1.44 × 1010 and 6.12 × 109 cfu/mL). Python‐based automatic feeding, based on online exhaust CO2 detection, was developed and implemented in 5 and 50 L cultivations. Compared to manual feeding, cell and spore quantities increased by 31.25% and 97.7%, respectively. Substituting costly peptone (fish) with NH4Cl reduced spore quantity by 8.46% and 9.25% in 5 and 50 L cultivations, while cutting nitrogen source cost by 87.08% and 91.39%. Employing exhaust CO2 concentration for automatic nitrogen supplementation effectively promotes Bacillus growth and spore formation.

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Enhanced sporulation of B. licheniformis BF‐002 through automatic co‐feeding of carbon and nitrogen sources

Ding,

Liu,

Hou

et al. 2024

Biotech & Bioengineering

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Bacillus licheniformis formulations are effective for environmental remediation, gut microbiota modulation, and soil improvement. An adequate spore quantity is crucial for the activity of B. licheniformis formulations. This study investigated the synergistic effects of carbon/nitrogen source consumption and concentration on B. licheniformis BF‐002 cultivation, with the aim of developing an automatic co‐feeding strategy to enhance spore production. Initial glucose (10 g/L) and amino nitrogen (1.5 g/L) concentrations promote cell growth, followed by reduced glucose (2.0 g/L) and amino nitrogen (0.5 g/L) concentrations for sustained spore generation. The spore quantity reached 2.59 × 1010 CFU/mL. An automatic co‐feeding strategy was developed and implemented in 5 and 50 L cultivations, resulting in spore quantities of 2.35 × 1010 and 2.86 × 1010 CFU/mL, respectively, improving by 6.81% and 30.00% compared to that with a fixed glucose concentration (10.0 g/L). The culture broth obtained at both the 5 and 50 L scales was spray‐dried, resulting in bacterial powder with cell viability rates of 85.94% and 82.68%, respectively. Even after exposure to harsh conditions involving high temperature and humidity, cell viability remained at 72.80% and 69.89%, respectively. Employing the automatic co‐feeding strategy increased the transcription levels of the spore formation‐related genes spo0A, spoIIGA, bofA, and spoIV by 7.42%, 8.46%, 8.87%, and 9.79%, respectively. The proposed strategy effectively promoted Bacillus growth and spore formation, thereby enhancing the quality of B. licheniformis formulations.

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Activation and thermal stabilization of a recombinant γ-glutamyltranspeptidase from Bacillus licheniformis ATCC 27811 by monovalent cations

Cited by 7 publications

References 90 publications

Evaluating Enzymatic Productivity—The Missing Link to Enzyme Utility

Evaluating Enzymatic Productivity—The Missing Link to Enzyme Utility

Enhancing Bacillus licheniformis sporulation with automatic nitrogen feeding based on online detection of exhaust CO₂

Enhanced sporulation of B. licheniformis BF‐002 through automatic co‐feeding of carbon and nitrogen sources

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Activation and thermal stabilization of a recombinant γ-glutamyltranspeptidase from Bacillus licheniformis ATCC 27811 by monovalent cations

Cited by 7 publications

References 90 publications

Evaluating Enzymatic Productivity—The Missing Link to Enzyme Utility

Evaluating Enzymatic Productivity—The Missing Link to Enzyme Utility

Enhancing Bacillus licheniformis sporulation with automatic nitrogen feeding based on online detection of exhaust CO2

Enhanced sporulation of B. licheniformis BF‐002 through automatic co‐feeding of carbon and nitrogen sources

Contact Info

Product

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Enhancing Bacillus licheniformis sporulation with automatic nitrogen feeding based on online detection of exhaust CO₂