Activation-induced cellular accumulation of histamine in immature but not mature murine mast cells

Abstract

Mast cell activation involves the rapid release of inflammatory mediators, including histamine, from intracellular granules. The cells are capable of regranulation and multiple rounds of activation. The goal of this study was to determine if there are changes in the content of pre-formed mast cell mediators after a round of activation. After 24 h, the histamine content of bone marrow-derived mast cells (BMMC), but not that of peritoneal mast cells, exceeded the amount in resting cells. Accumulation of histamine in BMMC peaked at 72 h of activation, and returned toward preactivation levels by 96 h. The increase in histamine content was accompanied by an increase in the gene expression of histidine decarboxylase. No increases in beta hexosaminidase or murine mast cell protease-6 were observed. These findings indicate that BMMC respond to activation by increasing total cell-associated histamine content. This increase may be important to the response of these cells upon subsequent exposure to antigens.

Central Nervous System Mast Cells in Peripheral Inflammatory Nociception

“…This suggests a potential contribution of preformed mediators, but a much stronger contribution of newly formed mediators. One hour stimulation of mast cells with TNP is sufficient to induce degranulation, release of histamine and other preformed mediators [26]. Intrathecal histamine administration is pro-nociceptive in mice [27] and spinal histamine receptor antagonists show anti-nociceptive efficacy in various animal models [28].…”

Structural features of mammalian histidine decarboxylase reveal the basis for specific inhibition

“…, 2006; Ai et al. , 2007), but not much is known of this process in other cell types where HDC expression seems to be associated with poorly‐characterized cell differentiation processes and/or subject to alterations in the control of cell proliferation (Fitz et al. , 2008; Tachibana et al.…”

Histamin als Immunmodulator

“…Sonobe et al (2004) ermittelten einen Histamingehalt von etwa 5-10 ng/ml in den Zellkulturüber-ständen muriner T-Zellen (bei einer eingesetzten Zellzahl von 10 7 /ml) 48 h nach der Stimulation mit dem Mitogen. Im Vergleich dazu setzen murine Mastzellen nach Ionomycin-induzierter Degranulation mehr als die 1000-fache Menge frei [39]. Auch Keratinozyten, die > 90 % der epidermalen Zellpopulation ausmachen, können Histamin bilden, wenn auch nur in geringen Mengen [40].…”