Activation of 3-O-methyl-glucose transport in rat thymus lymphocytes by concanavalin A temperature and calcium ion dependence and sensitivity to puromycin but not to cycloheximide

Yasmeen, D.; Laird, Alan; Hume, David; Weidemann, Maurice J.

doi:10.1016/0304-4165(77)90049-6

Cited by 46 publications

(17 citation statements)

References 23 publications

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“…The mechanism by which this increase in thymocyte cAMP concentration is brought about is uncertain, but is the subject of ongoing experiments. 4 The evidence upon which this hypothesis is based is consistent with the criteria generally accepted as evidence for a cAMP-mediated effect. First, T3 increased thymocyte cAMP concentration in a dosedependent manner.…”

Section: Effects Of Alprenololsupporting

confidence: 77%

“…6). Epinephrine did not affect the cAMP concentration in the suspend- 4 Stimulation of 2-DG uptake by Bt2cAMP in rat thymocytes: lack of calcium dependence. Cells were incubated in a standard medium (0) or in calcium-free (0) ing medium, measured at the end of incubation periods.…”

Section: Effects Of Adrenergic Agentsmentioning

confidence: 90%

“…Both calcium and cyclic (c) AMP have been implicated in the regulation of the uptake of sugar in various tissues (4)(5)(6)(7)(8)(9)(10). In addition, thyroid hormone has been demonstrated to affect both calcium (11)(12)(13) and cAMP (14)(15)(16)(17)(18) metabolism in different tissues.…”

Section: Introductionmentioning

confidence: 99%

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Studies of the mechanism by which 3,5,3'- triiodothyronine stimulates 2-deoxyglucose uptake in rat thymocytes in vitro. Role of calcium and adenosine 3':5'-monophosphate.

Segal¹,

Ingbar²

1981

J. Clin. Invest.

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A B S T R A C T The present experiments were designed to explore the mechanism whereby 3,5,3'-triiodothyronine (T3) stimulates the uptake of 2-deoxy-D-glucose (2-DG) in rat thymocytes in vitro. Addition of T3 evoked a transient, dose-related increase in cellular cyclic (c) AMP concentrations, evident within 5 min, followed soon by an increase in 2-DG uptake. The effects of T3 on both cAMP concentration and 2-DG uptake were dependent upon the presence of extracellular calcium. Epinephrine also induced a sequential increase in thymocyte cAMP concentration and 2-DG uptake. These responses were more prompt than those to T3, but were calcium independent. As with their combined effects on 2-DG uptake, T3 and epinephrine produced synergistic or additive effects on cellular cAMP concentration. Dibutyryl cAMP also stimulated 2-DG uptake, an effect that was more prompt than that of epinephrine, and, like that of epinephrine, was calcium independent.Prior or simultaneous addition of L-alprenolol (10 AM), which, we have previously shown, blocks the effect of both T3 and epinephrine on 2-DG uptake, also blocked the increase in thymocyte cAMP concentration induced by these agents. In contrast, Lalprenolol failed to block the increase in 2-DG uptake produced by dibutyryl cAMP. On the basis of these observations we suggest that T3 increases 2-DG uptake in the rat thymocyte by increasing the cellular concentration of cAMP, which then acts to enhance sugar transport. The increase in 2-DG uptake induced by epinephrine is also mediated by an increase in cAMP concentration. The greater

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Section: Effects Of Alprenololsupporting

confidence: 77%

Section: Effects Of Adrenergic Agentsmentioning

confidence: 90%

See 1 more Smart Citation

Studies of the mechanism by which 3,5,3'- triiodothyronine stimulates 2-deoxyglucose uptake in rat thymocytes in vitro. Role of calcium and adenosine 3':5'-monophosphate.

Segal¹,

Ingbar²

1981

J. Clin. Invest.

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“…Thus hexokinase activity may not be indicative of the maximum capacity of glycolysis in colonocytes. This rate ofglucose utilization is considerably higher than that reported for thymoctyes (Culvenor & Weidemann, 1976;Yassmeen et al, 1977;Hume et al, 1978;Brand et al, 1984), lymphocytes (Ardawi & Newsholme, 1983a, 1984a), but about 50%O lower than that reported for enterocytes (Watford et al, 1979;Porteous, 1980). About 83 % of the glucose removed was accounted for as lactate and small amounts of pyruvate.…”

Section: Glucose Metabolismmentioning

confidence: 53%

Fuel utilization in colonocytes of the rat

Ardawi¹,

Newsholme²

1985

Biochemical Journal

195

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1. In incubated colonocytes isolated from rat colons, the rates of utilization 02 glucose or glutamine were linear with respect to time for over 30 min, and the concentrations of adenine nucleotides plus the ATP/ADP or ATP/AMP concentration ratios remained approximately constant for 30 min. 2. Glutamine, n-butyrate or ketone bodies were the only substrates that caused increases in 02 consumption by isolated incubated colonocytes. 3. The maximum activity of hexokinase in colonic mucosa is similar to that of 6-phosphofructokinase. Starvation of the donor animal decreased the activities of hexokinase and 6-phosphofructokinase, whereas it increased those of glucose-6-phosphatase and fructose-bisphosphatase. 4.Isolated incubated colonocytes utilized glucose at about 6.8 #mol/min per g dry wt., with lactate accounting for 83% of glucose removed. These rates were not affected by the addition of glutamine, acetoacetate or n-butyrate, and starvation of the donor animal. 5. Isolated incubated colonocytes utilized glutamine at about 5.5 umol/min per g dry wt., which is about 21 % of the maximum activity of glutaminase. The major end-products of glutamine metabolism were glutamate, aspartate, alanine and ammonia. Starvation of the donor animal decreased the rate of glutamine utilization by colonocytes, which is accompanied by a decrease in glutamate formation and in the maximum activity of glutaminase. 6. Isolated incubated coloncytes utilized acetoacetate at about 3.5 ,mol/min per g dry wt. This rate was not markedly affected by addition of glucose or by starvation of the donor animal. 7. When colonocytes were incubated with n-butyrate, both acetoacetate and 3-hydroxybutyrate were formed, with the latter produced.

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“…Several groups of investigators have shown that one of the earliest effects of the lectin binding to the lymphocyte is the enhancement in glucose transport (1)(2)(3)(4)(5)(6)(7)(8). Peters and Hausen (1) have shown that increase in 3-0-methyl glucose (3-OMG)' uptake in lymphocytes was demonstrable within minutes of exposure to PHA and is independent of de novo protein synthesis.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of mitogen-induced stimulation of glucose transport in human peripheral blood mononuclear cells. Evidence of an intracellular reserve pool of glucose carriers and their recruitment.

Jacobs¹,

Lee²,

Jung³

et al. 1989

J. Clin. Invest.

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The present study examines the effects of phytohemagglutinin stimulation of a population of human (h) PBMC Phytohemagglutinin-treatment increases the plasma membrane pool size of M-sites with a concomitant reduction in the microsomal pool size without affecting the binding affinities or the total number of M-sites/cell. Data presented in this paper demonstrate that there a-e two pools of glucose transporters in these cells and phytohemagglutinin stimulation induces an energy-dependent net translocation of glucose transporters from an intracellular reserve pool to the plasma membrane, which accounts for > 60% of the increment in glucose transport.

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Activation of 3-O-methyl-glucose transport in rat thymus lymphocytes by concanavalin A temperature and calcium ion dependence and sensitivity to puromycin but not to cycloheximide

Cited by 46 publications

References 23 publications

Studies of the mechanism by which 3,5,3'- triiodothyronine stimulates 2-deoxyglucose uptake in rat thymocytes in vitro. Role of calcium and adenosine 3':5'-monophosphate.

Studies of the mechanism by which 3,5,3'- triiodothyronine stimulates 2-deoxyglucose uptake in rat thymocytes in vitro. Role of calcium and adenosine 3':5'-monophosphate.

Fuel utilization in colonocytes of the rat

Mechanism of mitogen-induced stimulation of glucose transport in human peripheral blood mononuclear cells. Evidence of an intracellular reserve pool of glucose carriers and their recruitment.

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