Activation of a 74 kDa plasma membrane protein kinase by hyperosmotic shocks in the halotolerant alga Dunaliella salina

Chitlaru, Edith; Seger, Rony; Pick, Uri

doi:10.1016/s0176-1617(97)80007-7

Cited by 9 publications

(5 citation statements)

References 20 publications

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“…It has also been reported that drought or high salinity also activates SIMK (stress-induced MAPK) in Medicago sativa cells and SIPK (salicylic acid-induced protein kinase) in tobacco cells. Chitlaru et al found that hypertonic stress could rapidly activate a protein kinase, and confirmed that the protein kinase belonged to MEK1 [217]. Xiong et al found that the OsMAPK5 gene in rice was induced by a variety of biological and abiotic stresses, and overexpression of this gene in rice could enhance drought resistance, salt resistance and low-temperature tolerance of transgenic rice [218].…”

Section: Signal Transduction Related Genesmentioning

confidence: 99%

Response Mechanism of Plants to Drought Stress

et al. 2021

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With the global climate anomalies and the destruction of ecological balance, the water shortage has become a serious ecological problem facing all mankind, and drought has become a key factor restricting the development of agricultural production. Therefore, it is essential to study the drought tolerance of crops. Based on previous studies, we reviewed the effects of drought stress on plant morphology and physiology, including the changes of external morphology and internal structure of root, stem, and leaf, the effects of drought stress on osmotic regulation substances, drought-induced proteins, and active oxygen metabolism of plants. In this paper, the main drought stress signals and signal transduction pathways in plants are described, and the functional genes and regulatory genes related to drought stress are listed, respectively. We summarize the above aspects to provide valuable background knowledge and theoretical basis for future agriculture, forestry breeding, and cultivation.

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Section: Signal Transduction Related Genesmentioning

confidence: 99%

Response Mechanism of Plants to Drought Stress

et al. 2021

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“…Salt-induced changes in organellar membranes, such as the expression of ER fatty acid elongase, were also reported (7). High salinity also affects sodium transport (8), lipid organization (9,10), and activation of PM protein kinases (11). Taken together, the accumulated evidence suggested that short and long range reorganization of the structure and composition of the PM is at the core of molecular mechanisms responsible for the salinity response.…”

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confidence: 90%

Salt-induced Changes in the Plasma Membrane Proteome of the Halotolerant Alga Dunaliella salina as Revealed by Blue Native Gel Electrophoresis and Nano-LC-MS/MS Analysis

Katz

Waridel

Shevchenko

et al. 2007

Molecular & Cellular Proteomics

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The halotolerant alga Dunaliella salina is a recognized model photosynthetic organism for studying plant adaptation to high salinity. The adaptation mechanisms involve major changes in the proteome composition associated with energy metabolism and carbon and iron acquisition. To clarify the molecular basis for the remarkable resistance to high salt, we performed a comprehensive proteomics analysis of the plasma membrane. Plasma membrane proteins were recognized by tagging intact cells with a membrane-impermeable biotin derivative. Proteins were resolved by two-dimensional blue native/SDS-PAGE and identified by nano-LC-MS/MS. Of 55 identified proteins, about 60% were integral membrane or membraneassociated proteins. We identified novel surface coat proteins, lipid-metabolizing enzymes, a new family of membrane proteins of unknown function, ion transporters, small GTP-binding proteins, and heat shock proteins. The abundance of 20 protein spots increased and that of two protein spots decreased under high salt. The major salt-regulated proteins were implicated in protein and membrane structure stabilization and within signal transduction pathways. The migration profiles of native protein complexes on blue native gels revealed oligomerization or co-migration of major surface-exposed proteins, which may indicate mechanisms of stabilization at high salinity. Molecular & Cellular Proteomics 6:1459 -1472, 2007.The halotolerant alga Dunaliella salina uses a unique osmoregulatory mechanism and is able to proliferate in environments with extreme salt content (1). This is achieved through remarkable changes in metabolism and ion transport brought about by up-regulation or down-regulation of multiple enzymes and membrane proteins. Elucidation of the molecular basis for this remarkable adaptation might provide tools to improve the resistance of crop plants to the progressive salinization of soils, which is already a major limitation for agricultural productivity worldwide. By characterizing the changes in the soluble subproteome of D. salina, we demonstrated previously that the alga responds to hypersaline conditions by up-regulating key enzymes in photosynthetic CO 2 fixation and in energy metabolism that divert carbon metabolism to massive synthesis of glycerol, the osmotic element in Dunaliella (2).Salinity stress destabilizes biological membranes and affects the solubility of many essential substrates and ions (3). Adaptation to salinity stress might also alter the composition and organization of the membrane proteome associated with the stabilization and enhancement of ion transporters. Early studies revealed the accumulation of two carbonic anhydrases, dCAI 1 and dCAII (4, 5), and a transferrin-like protein (6) that presumably compensated the impaired availability of bicarbonate and iron under high salt. Salt-induced changes in organellar membranes, such as the expression of ER fatty acid elongase, were also reported (7). High salinity also affects sodium transport (8), lipid organization (9, 10), and activation of PM pr...

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“…The LAP kinase phosphorylated MBP and histone in vitro, similarly to MAP kinases, but although its activation was phosphorylation-dependent, it was not recognized by anti-phosphotyrosine antibodies. Hyperosmotic stress also led to a transient activation of a 74 kDa plasma membrane protein kinase, most probably identi®ed as a MAP kinase kinase (Chitlaru et al, 1997). In higher plants, very little information is available regarding osmotically induced kinase activities.…”

Section: Introductionmentioning

confidence: 99%

MAP kinase activation by hypoosmotic stress of tobacco cell suspensions: towards the oxidative burst response?

et al. 1999

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SummaryHypoosmotic stress activates a phosphorylationdependent oxidative burst. In-gel kinase assays were performed to characterize the protein kinases that could be implicated in osmoregulation and in the activation of the oxidative burst. Hypoosmotic stress activated several kinases among which 50 and 46 kDa proteins displayed mitogen-activated protein kinase (MAP kinase) properties. They phosphorylated myelin basic protein in the absence of calcium, were recognized by antibodies directed against human MAP kinases, and were phosphorylated on tyrosine. Immunoprecipitation with an antibody directed against the tobacco MAP kinase Ntf4 showed that at least one of the activated kinases would be Ntf4-like. Apigenin, a MAP kinase and cyclindependent kinase inhibitor which prevents the hypoosmotically induced oxidative burst (Cazale Â et al., 1998; Plant Physiol. 116, 659±669), inhibited these kinases in vitro suggesting that they may play a role in the activation of the oxidative burst. Like the oxidative response, activation of the kinases depended on extracellular calcium in¯ux and protein kinases sensitive to staurosporine and 6-DMAP. However, kinase activation did not depend on ef¯uxes through anion channels or on the oxidative burst. Two-dimensional in-gel kinase assays revealed the presence of three protein kinases with an apparent molecular mass of 50 kDa and one of 46 kDa, all four being activated by hypoosmotic stress. The same kinases were also activated by oligogalacturonides and salicylic acid, underlying the importance of these MAP kinases as common components of different signaling pathways triggered by different extracellular stimuli.

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Activation of a 74 kDa plasma membrane protein kinase by hyperosmotic shocks in the halotolerant alga Dunaliella salina

Cited by 9 publications

References 20 publications

Response Mechanism of Plants to Drought Stress

Response Mechanism of Plants to Drought Stress

Salt-induced Changes in the Plasma Membrane Proteome of the Halotolerant Alga Dunaliella salina as Revealed by Blue Native Gel Electrophoresis and Nano-LC-MS/MS Analysis

MAP kinase activation by hypoosmotic stress of tobacco cell suspensions: towards the oxidative burst response?

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