Activation of autophagy in periodontal ligament mesenchymal stem cells promotes angiogenesis in periodontitis

Wei, Wei; An, Yulin; An, Yu; Fei, Dongdong; Wang, Qintao

doi:10.1002/jper.17-0341

Cited by 60 publications

(57 citation statements)

References 35 publications

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“…Mesenchymal stem cells (MSCs) have been widely reported to be associated with angiogenesis, including dental stem cells [4,8,9]. Periodontal ligament stem cells (PDLSCs) are a class of odontogenic MSCs residing in periodontal ligament with functions of self-renewal, multi-directional differentiation potential and cellular regulation [10].…”

Section: Ivyspringmentioning

confidence: 99%

“…Periodontal ligament stem cells (PDLSCs) are a class of odontogenic MSCs residing in periodontal ligament with functions of self-renewal, multi-directional differentiation potential and cellular regulation [10]. Recent study reported that PDLSCs had an ability of angiogenic promotion [4,9], whereas the underlying mechanism of PDLSCs on angiogenesis under inflammatory micro-environment needed to be uncovered.…”

Section: Ivyspringmentioning

confidence: 99%

“…Anomalous vascularization is one of the most common characteristics in periodontitis leading to progressive inflammation, which results from disrupted angiogenesis in the periodontal tissues [3]. Recently, large numbers of studies on periodontitis have been concentrating on angiogenesis in the inflammatory tissues [4]. Increased blood vessels were observed in gingiva of the periodontitis as a result of enhanced inflammatory infiltration [5,6].…”

Section: Introductionmentioning

confidence: 99%

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PDLSCs Regulate Angiogenesis of Periodontal Ligaments via VEGF Transferred by Exosomes in Periodontitis

Zhang

Zhou

et al. 2020

Int. J. Med. Sci.

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Abnormal angiogenesis is one of the significant features in periodontitis leading to progressive inflammation, but angiogenic changes of periodontal ligaments under inflammatory condition were rarely reported. Periodontal ligament stem cells (PDLSCs) were a kind of dental stem cells associated with vascularization. Here we investigated the alteration of angiogenesis of periodontal ligament in periodontitis, and revealed an exosome-mediated pathway to support the effect of PDLSCs on angiogenic improvement. Vascular specific marker CD31 and VEGFA were found to be highly expressed in periodontal ligaments of periodontitis. The VEGFA expression was up-regulated in inflamed PDLSCs compared to control, meanwhile the tube formation of HUVECs was improved when co-cultured with inflamed PDLSCs. Exosomes secretion of PDSLCs was augmented by inflammation, and promoted angiogenesis of HUVECs, whereas blocking secretion of exosomes led to degenerated angiogenesis of HUVECs. Exosome-trasferred VEGFA was proven to be the crucial communicator between PDLSCs and HUVECs. Inflammation inhibited miR-17-5p expression of PDLSCs and relieved its target VEGFA. However, overexpression of miR-17-5p blocked the pro-angiogenic ability of inflamed PDLSCs. In conclusion, the findings indicated that vascularization of periodontal ligaments was enhanced, and inflammatory micro-environment of periodontitis facilitated pro-angiogenesis of PDLSCs through regulating exosome-mediated transfer of VEGFA, which was targeted by miR-17-5p.

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Section: Ivyspringmentioning

confidence: 99%

Section: Ivyspringmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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PDLSCs Regulate Angiogenesis of Periodontal Ligaments via VEGF Transferred by Exosomes in Periodontitis

Zhang

Zhou

et al. 2020

Int. J. Med. Sci.

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“…Conversely, angiogenesis was inhibited when the autophagy was suppressed via silencing Beclin‐1, an important factor in autophagy flux. These findings suggested that inflammation‐related autophagy in PDLSCs might regulate the angiogenesis ability in periodontium (Wei, An, An, Fei, & Wang, ).…”

Section: Autophagy and Periodontal Inflammationmentioning

confidence: 98%

The role of autophagy in the pathogenesis of periodontal disease

Jiang

Zhu

2019

Oral Diseases

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Periodontal disease is a chronic inflammatory disease leading to destruction of periodontal tissues. As a local inflammation, periodontopathic bacterium, pro‐inflammatory mediators, and local immune response play pivotal role in the progress of periodontal disease. Besides, cigarette smoke has long been associated with periodontal disease and tooth loss. Autophagy is an intracellular degradation process highly conserved from yeast to humans. As a lysosomal degradation pathway of self‐digestion, it is critical for maintaining cells homeostasis and development. The role of autophagy has been investigated in oral diseases, such as oral cancer, periapical lesions, and oral candidiasis. Recently, increasing studies investigated the role of autophagy in periodontal disease. In this review, we try to illustrate the effect of autophagy on periodontal disease pathogenesis from 5 aspects: autophagy affects the intracellular infection and survival of bacteria; autophagy has an interaction with periodontal inflammation; autophagy is pivotal in periodontal cells biology and periodontal tissues destruction and reconstruction; autophagy can be induced by cigarette smoke; last but not least, autophagy may affect periodontal disease via endoplasmic reticulum stress.

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“…Autophagy is a biological process by which autophagosomes are formed and then fused with lysosomes to degrade autophagic contents by lysosomal protease. Autophagy can promote angiogenesis under inflammatory stimulation, hypoxia or high-glucose microenvironments [139,140]. Transcription factor EB (TFEB) is a master regulator of autophagy and lysosomal biogenesis.…”

Section: Inducing Autophagymentioning

confidence: 99%

Insights into the angiogenic effects of nanomaterials: mechanisms involved and potential applications

Liu

Zhang

et al. 2020

J Nanobiotechnol

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The vascular system, which transports oxygen and nutrients, plays an important role in wound healing, cardiovascular disease treatment and bone tissue engineering. Angiogenesis is a complex and delicate regulatory process. Vascular cells, the extracellular matrix (ECM) and angiogenic factors are indispensable in the promotion of lumen formation and vascular maturation to support blood flow. However, the addition of growth factors or proteins involved in proangiogenic effects is not effective for regulating angiogenesis in different microenvironments. The construction of biomaterial scaffolds to achieve optimal growth conditions and earlier vascularization is undoubtedly one of the most important considerations and major challenges among engineering strategies. Nanomaterials have attracted much attention in biomedical applications due to their structure and unique photoelectric and catalytic properties. Nanomaterials not only serve as carriers that effectively deliver factors such as angiogenesis-related proteins and mRNA but also simulate the nano-topological structure of the primary ECM of blood vessels and stimulate the gene expression of angiogenic effects facilitating angiogenesis. Therefore, the introduction of nanomaterials to promote angiogenesis is a great helpful to the success of tissue regeneration and some ischaemic diseases. This review focuses on the angiogenic effects of nanoscaffolds in different types of tissue regeneration and discusses the influencing factors as well as possible related mechanisms of nanomaterials in endothelial neovascularization. It contributes novel insights into the design and development of novel nanomaterials for vascularization and therapeutic applications.

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Activation of autophagy in periodontal ligament mesenchymal stem cells promotes angiogenesis in periodontitis

Abstract: Autophagy modulates angiogenesis-promoting ability of PDLSCs, which could be increased by an inflammatory environment.

Cited by 60 publications

References 35 publications

PDLSCs Regulate Angiogenesis of Periodontal Ligaments via VEGF Transferred by Exosomes in Periodontitis

PDLSCs Regulate Angiogenesis of Periodontal Ligaments via VEGF Transferred by Exosomes in Periodontitis

The role of autophagy in the pathogenesis of periodontal disease

Insights into the angiogenic effects of nanomaterials: mechanisms involved and potential applications

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