Activation of Bacterial Cytochrome P450 in the Intestine of Larval Mosquitoes an Indication of Its Role in the Insecticide Resistance

Abstract

Mosquitoes apply very important impact on people life, and their control, day by day, is getting harder and harder. This difficulty comes from different issues such as insecticide resistance. The gut microbiome (GM) in different animals has been discovered to add principle influence on the gastrointestinal tract physiology. For example, GM participates in degradation of some poisonus chemicals into safer molecules that could be handled by the animal host. Eukaryotic cytochrome P450 has been detected to inseret some degradation processes in other animal models such as zebra fish. However, these effects are not yet known in mosquitos. In order to stand on these problems and find if the prokaryotic P450 is involved, this study had been done to find out if the GM has any role in developing such resistance in mosquitos. Larvae were used in this experiment which exposed to benzothiozole (BZT) to trigger the hypothetical role of prokaryotic P450. By using polymerase chain reaction (PCR), the result indicates that the bacterial P450 was initiated and might be involved in the BZT exposure handling.

The Role of Gut Bacterial Cytochrome-P450 of Mosquito Larvae in Degradation of Temephosas Insecticide

“…The investigation raising conditions and techniques were adaptedfrom ( 18).The instar larvae were collected from field mosquitos. Here, a group of 50 larvae of Aedesalbopictusweresubjected to temephos at 0.5 mg/l and ketoconazole, an inhibitor of the bacterial cytochrome P450, at 200mg/50 liter (TKG), 50 larvae received ketoconazole only at 200mg/50 liter (KG), and 50 larvae that played as a control group (CG) were only subjected to the larvicide.…”

“…These enzymes play important roles in degrading toxins and chemical poisons in the environment (12-16, 17). Previously, the P450 was detected to be present in the gut bacteria of mosquito larvae after the use of a certain chemical encouraging changes in the gut bacteria from non-P450-bearing bacteria to P450-bearing bacteria ( 18), and this triggered the current work hypothesis that bacteria may protect larvae via bacterial-P450 degradation of larvicides.…”