Activation of CD38 by Interleukin-8 Signaling Regulates Intracellular Ca2+ Level and Motility of Lymphokine-activated Killer Cells

Abstract

CD38 is an ADP-ribosyl cyclase, producing a potent

Doxorubicin-induced reactive oxygen species generation and intracellular Ca2+increase are reciprocally modulated in rat cardiomyocytes

“…] i Levels of [Ca 2+ ]i in quiescent isolated adult rat cardiomyocytes were measured as described previously (Xie et al, 2003;Rah et al, 2005). Briefly, cardiomyocytes attached to laminine-coated plates were loaded with 5 µM Fluo 3-AM (Molecular Probe, Eugene, OR) in KH buffer containing 1% BSA at 37 o C for 30 min, and the plates were washed three times with KH buffer and used for the treatment with various drugs.…”

Oxytocin-induced elevation of ADP-ribosyl cyclase activity, cyclic ADP-riboseor Ca2+ concentrations is involved in autoregulation of oxytocin secretionin the hypothalamus and posterior pituitary in male mice

“…Intracellular cADPR concentrations are known to be regulated in many different ways: in one such mechanism, ADP-ribosyl cyclase or CD38 seems to be coupled directly with neurotransmitter or hormone receptors such as muscarinic acetylcholine or metabotropic glutamate receptors via different G proteins on the membrane surface (Higashida et al, 1997(Higashida et al, , 1999(Higashida et al, , 2007; or phosphorylation downstream of the G-protein-coupled receptor signaling pathway (Boittin et al, 2003;Sternfeld et al, 2003). Specifically, the activation of ADP-ribosyl cyclase or CD38 by cyclic GMP-or cyclic AMP-dependent protein kinases has been reported in Aplysia californica (Graeff et al, 1998), LAK cells (Rah et al, 2005) and artery smooth muscle cells (Boittin et al, 2003). However, there have been no previous reports regarding the mechanisms by which ADP-ribosyl cyclase and CD38 are activated after OT receptor stimulation in the hypothalamus, leading to secretion of OT.…”

Kidney ADP-Ribosyl Cyclase Inhibitors as a Therapeutic Tool for Diabetic Nephropathy

“…ADPR-cyclase generates two important Ca 2+ -mobilizing second messengers, cADPR and NAADP, from NAD + and NADP + , respectively (37)(38)(39). The second messenger, cADPR, increases intracellular Ca 2+ concentration ([Ca 2+ ]i) through the release of Ca 2+ from intracellular endoplasmic reticulum (ER) stores via ryanodine receptors and/or Ca 2+ influx through plasma membrane Ca 2+ channels (5,39,40,41). The other second messenger, NAADP, increases intracellular Ca 2+ concentration ([Ca 2+ ]i) through the release of Ca 2+ from a discrete intracellular store, called acidic organelles, via Twopore channels (TPCs) (42).…”

“…ADPR-cyclase-involved GPCRs include the -adrenergic receptor, muscarinic receptor, interleukin 8 receptor (IL8R) and AT1R. The mechanism by which GPCR activates ADPRcyclase was discovered from the functional loop involving IL-8 and CD38 in lymphokineactivated killer (LAK) cells (5 (Figure 2). …”

“…Mounting evidence has indicated that G protein-coupled receptors, including the angiotensin II (Ang II) receptor, mediate activation of ADPR-cyclase to generate Ca 2+ signaling messengers (3)(4)(5). We have studied Ang II receptor-mediated activation of ADPR-cyclase, resulting in Ca 2+ dysfunction Fig.…”