Activation of Cellulose Membranes with 1,1′-Carbonyldiimidazole or 1-Cyano-4-dimethylaminopyridinium tetrafluoroborate as a Basis for the Development of Immunosensors

The effect of immobilization of thrombin inhibitors onto self-assembled monolayers on the adsorption and activity of thrombin

“…This strategy avoid the presence of some carbonyl groups on the surface of the monolayer that could induce non-specific protein adsorption [36].…”

Co-immobilization of glucose oxidase and hexokinase on silicate hybrid sol–gel membrane for glucose and ATP detections

“…The activity of the immobilized GOD could be measured using a photometric activity assay via the produced H 2 O 2 from a peroxidase coupled coloring reaction (Stöllner et al, 2002). This measurement revealed that 0.4 mU GOD was immobilized on the electrode surface.…”

“…The apparent enzyme activities of the entrapped GOD were determined via the produced H 2 O 2 by a coupled peroxidase color reaction (Stöllner et al, 2002). Photometric measurements were performed with a Cary 5E UV-vis-NIR spectrophotometer.…”

Specific binding of glycoproteins with poly(aniline boronic acid) thin film

“…Furthermore, the activity of the bound and released GOx was determined via the produced H 2 O 2 by a coupled peroxidase color reaction [25]. Our measurements reveal that 0.075 nU of the bound GOx is released by the incubation in the sorbitol solution and 0.188 nU of GOx still remains on the modified GC electrode, $ 28.5% of the total bound GOx is replaced by sorbitol.…”

“…The apparent enzyme activities of bound and released GOx were determined from the produced H 2 O 2 by a coupled peroxidase color reaction [25]. To determine the released GOx, first we incubated the GOx-modified electrode in 300 lL of 0.1 M sorbitol + 0.1 M pH 7.5 PBS for 20 min and then removed the electrode; then we added 700 lL of 0.1 M pH 5.0 PBS containing 0.1 mg mL À1 TMB + 50 mM D(+)glucose + 25 lg ml À1 HRP into the incubated solution and rapidly mixed the solution.…”