Activation of chicken gamma-delta T lymphocytes by a purified ulvan extract

Guriec, Nathalie; Bussy, Frédérick; Gouin, Christelle; Mathiaud, Olivier; Goff, Matthieu Le; Delarue, Jacques; Collén, Pi Nyvall

doi:10.1016/j.vetimm.2021.110255

Cited by 5 publications

(3 citation statements)

References 45 publications

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“…On the contrary, other studies demonstrated immune-stimulating effects for fucoidan [45,55] and alginate oligosaccharides [56,57]. For green macroalgae, the sulfated polysaccharide ulvan has shown immune-stimulating effects providing increased protection against infection when used as ingredients in aquafeed or husbandry animal feed [58][59][60][61]. In cell models treated with Ulva polysaccharides, an increase in inflammatory cytokines has been observed [62,63].…”

Section: Discussionmentioning

confidence: 99%

“…Both ulvan and fucoidan have been shown to activate inflammatory signaling [24,45]. For ulvan, it has further been proposed that this effect is mediated through the PRRs toll-like receptor 2 (TLR2) and TLR4, where also LPS binds to initiate the inflammatory signaling cascade [56,58,59]. Activation of IRFs through PRRs depends on the endocytosis of the receptor [77,78] and it would be interesting to see if binding of specific macroalgae polysaccharides to the receptor might inhibit this mechanism.…”

Section: Discussionmentioning

confidence: 99%

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Green (Ulva fenestrata) and Brown (Saccharina latissima) Macroalgae Similarly Modulate Inflammatory Signaling by Activating NF-κB and Dampening IRF in Human Macrophage-Like Cells

Mildenberger,

Rebours

2024

Journal of Immunology Research

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Macroalgae are considered healthy food ingredients due to their content in numerous bioactive compounds, and the traditional use of whole macroalgae in Asian cuisine suggests a contribution to longevity. Although much information is available about the bioactivity of pure algal compounds, such as different polyphenols and polysaccharides, documentation of potential effects of whole macroalgae as part of Western diets is limited. Lifestyle- and age-related diseases, which have a high impact on population health, are closely connected to underlying chronic inflammation. Therefore, we have studied crude extracts of green (Ulva fenestrata) and brown (Saccharina latissima) macroalgae, as two of the most promising food macroalgae in the Nordic countries for their effect on inflammation in vitro. Human macrophage-like reporter THP-1 cells were treated with macroalgae extracts and stimulated with lipopolysaccharide (LPS) to induce inflammatory signalling. Effects of the macroalgae extracts were assessed on transcription factor activity of NF-κB and IRF as well as secretion and/or expression of the cytokines TNF-α and IFN-β and chemokines IL-8 and CXCL10. The crude macroalgae extracts were further separated into polyphenol-enriched and polysaccharide-enriched fractions, which were also tested for their effect on transcription factor activity. Interestingly, we observed a selective activation of NF-κB, when cells were treated with macroalgae extracts. On the other hand, pretreatment with macroalgae extracts selectively repressed IRF activation when inflammatory signaling was subsequently induced by LPS. This effect was consistent for both tested species as well as for polyphenol- and polysaccharide-enriched fractions, of which the latter had more pronounced effects. Overall, this is the first indication of how macroalgae could modulate inflammatory signaling by selective activation and subsequent repression of different pathways. Further in vitro and in vivo studies of this mechanism would be needed to understand how macroalgae consumption could influence the prevention of noncommunicable, lifestyle- and age-related diseases that are highly related to unbalanced inflammatory processes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Green (Ulva fenestrata) and Brown (Saccharina latissima) Macroalgae Similarly Modulate Inflammatory Signaling by Activating NF-κB and Dampening IRF in Human Macrophage-Like Cells

Mildenberger,

Rebours

2024

Journal of Immunology Research

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“…Although the CD8α + antibody would have picked up a small subset of γδ T cells, the majority of γδ T cells (CD8 − ) were excluded from the mentioned study. Interestingly, others have reported that the majority of γδ T cells in peripheral blood express MHC class II even in a resting state, and increased MFI was shown in an in vivo experiment where chickens were provided high doses of Ulvan in their drinking water ( 162 ). CD25 is constitutively expressed on a subset of γδ T cells but is also described as an activation marker because frequencies of CD25 + cells within the γδ T population are increased upon activation with, e.g., Salmonella and Eimeria ( 124 , 157 ).…”

Section: T Cellsmentioning

confidence: 99%

Delineation of chicken immune markers in the era of omics and multicolor flow cytometry

Härtle,

Sutton,

Vervelde

et al. 2024

Front. Vet. Sci.

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Multiparameter flow cytometry is a routine method in immunological studies incorporated in biomedical, veterinary, agricultural, and wildlife research and routinely used in veterinary clinical laboratories. Its use in the diagnostics of poultry diseases is still limited, but due to the continuous expansion of reagents and cost reductions, this may change in the near future. Although the structure and function of the avian immune system show commonalities with mammals, at the molecular level, there is often low homology across species. The cross-reactivity of mammalian immunological reagents is therefore low, but nevertheless, the list of reagents to study chicken immune cells is increasing. Recent improvement in multicolor antibody panels for chicken cells has resulted in more detailed analysis by flow cytometry and has allowed the discovery of novel leukocyte cell subpopulations. In this article, we present an overview of the reagents and guidance needed to perform multicolor flow cytometry using chicken samples and common pitfalls to avoid.

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Natural polysaccharides protect against diet-induced obesity by improving lipid metabolism and regulating the immune system

Tang

Wang

Chen

et al. 2023

Food Research International

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Activation of chicken gamma-delta T lymphocytes by a purified ulvan extract

Cited by 5 publications

References 45 publications

Green (Ulva fenestrata) and Brown (Saccharina latissima) Macroalgae Similarly Modulate Inflammatory Signaling by Activating NF-κB and Dampening IRF in Human Macrophage-Like Cells

Green (Ulva fenestrata) and Brown (Saccharina latissima) Macroalgae Similarly Modulate Inflammatory Signaling by Activating NF-κB and Dampening IRF in Human Macrophage-Like Cells

Delineation of chicken immune markers in the era of omics and multicolor flow cytometry

Natural polysaccharides protect against diet-induced obesity by improving lipid metabolism and regulating the immune system

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