Activation of cytosolic phospholipase A2 in Her14 fibroblasts by hydrogen peroxide: a p42/44MAPK-dependent and phosphorylation-independent mechanism

Rossum, Gerda S.A.T. van; Drummen, Gregor P. C.; Verkleij, Arie J.; Post, Jan Andries; Boonstra, Johannes

doi:10.1016/j.bbalip.2003.12.008

Cited by 45 publications

(25 citation statements)

References 87 publications

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“…Although evidences exist that hydrogen peroxide activates p44/p42 MAPK in mammalian cells, our results show for the first time activation of p44/p42 MAPK by hydrogen peroxide in the yeast S. cerevisiae (Hannken et al, 2000;Nguyen et al, 2004;van Rossum et al, 2004). Interestingly Mkp1, a Pneumocystis carinii homologue of Mpk1, has been found to be activated by the same oxidant (Fox and Smulian, 1999).…”

Section: Discussionmentioning

confidence: 64%

Oxidative Stress ActivatesFUS1andRLM1Transcription in the YeastSaccharomyces cerevisiaein an Oxidant-dependent Manner

Staleva

Hall

Orlow

2004

MBoC

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Mating in haploid Saccharomyces cerevisiae occurs after activation of the pheromone response pathway. Biochemical components of this pathway are involved in other yeast signal transduction networks. To understand more about the coordination between signaling pathways, we used a "chemical genetic" approach, searching for compounds that would activate the pheromone-responsive gene FUS1 and RLM1, a reporter for the cell integrity pathway. We found that catecholamines (L-3,4-hydroxyphenylalanine [L-dopa], dopamine, adrenaline, and noradrenaline) elevate FUS1 and RLM1 transcription. N-Acetyl-cysteine, a powerful antioxidant in yeast, completely reversed this effect, suggesting that FUS1 and RLM1 activation in response to catecholamines is a result of oxidative stress. The oxidant hydrogen peroxide also was found to activate transcription of an RLM1 reporter. Further genetic analysis combined with immunoblotting revealed that Kss1, one of the mating mitogen-activated protein kinases (MAPKs), and Mpk1, an MAPK of the cell integrity pathway, participated in L-dopa-induced stimulation of FUS1 and RLM1 transcription. We also report that Mpk1 and Hog1, the high osmolarity MAPK, were phosphorylated upon induction by hydrogen peroxide. Together, our results demonstrate that cells respond to oxidative stress via different signal transduction machinery dependent upon the nature of the oxidant. INTRODUCTIONIn the haploid cells of the yeast Saccharomyces cerevisiae, four essential MAPK cascades (Figure 1) respond to different external signals to mediate specific responses. The mating pathway is activated by peptide pheromones and induces cell-cycle arrest and the morphological changes required for mating (Elion, 1998;Gustin et al., 1998). The invasive growth pathway is activated by starvation and induces foraging into the agar substratum. The high osmolarity glycerol (HOG) pathway increases intracellular glycerol levels in response to hypertonic stress, whereas the cell integrity pathway is activated by hypotonic stress, heat shock, or impaired cell wall synthesis. Recently, an additional MAPK cascade has been described: the Kss1 vegetative growth pathway (Lee and Elion, 1999). The Kss1 pathway may be activated by cell wall stress or changes in osmolarity (Cullen et al., 2000).The mating pathway is the most studied cellular response to an external signal. As a relatively simple G protein-coupled cascade, it is a widely used model to study mammalian G protein-coupled receptors. The mating cascade includes pheromone receptors (Ste2 and Ste3), G proteins (Gpa1, Ste4, and Ste18), the p21 activating protein kinase Ste20, a mitogen-activated protein kinase kinase kinase (MAPKKK) Ste11, a mitogen-activated protein kinase kinase (MAPKK) Ste7, a scaffolding protein Ste5 and two MAPKs (Fus3 and Kss1) (Gustin et al., 1998;Madhani and Fink, 1998;Farley et al., 1999). Targets of the terminal MAPK include Ste12, a factor required for transcription of pheromone-responsive genes, and Far1, a bifunctional protein required for polarization and G1...

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Section: Discussionmentioning

confidence: 64%

Oxidative Stress ActivatesFUS1andRLM1Transcription in the YeastSaccharomyces cerevisiaein an Oxidant-dependent Manner

Staleva

Hall

Orlow

2004

MBoC

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“…29 Accordingly, platelet incubation with atorvastatin downregulated PLA 2 activation, thus providing mechanistic insight into the in vitro inhibition of platelet TxB 2, which was detected in the present and previous studies. 5 Finally, the inhibition of reactive oxidant species could also explain the increase of platelet NOx observed 24 hours after atorvastatin administration.…”

Section: Antiplatelet Effect By Statinsmentioning

confidence: 59%

Immediate Antioxidant and Antiplatelet Effect of Atorvastatin via Inhibition of Nox2

et al. 2012

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Background-Statins exert an antithrombotic effect in patients at risk of or with acute thrombosis, but no study has investigated whether this effect is immediate and whether there is an underline mechanism. Methods and Results-Patients with hypercholesterolemia were randomly allocated to a Mediterranean diet with low cholesterol intake (Ͻ300 mg/d; nϭ15) or atorvastatin (40 mg/d; nϭ15). Oxidative stress, as assessed by serum Nox2 and urinary isoprostanes, and platelet activation, as assessed by platelet recruitment, platelet isoprostanes, and thromboxane A 2 , platelet Nox2, Rac1, p47

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“…For instance, Min/ϩ and WT enterocytes may differ in levels of phospholipase A 2 (PLA 2 ) activity, leading to differences in liberated AA, the substrate for conversion to PGE 2 by cyclooxygenases. Oxidative stress in Min/ϩ intestinal tissue may induce cPLA 2 activation via a mitogen-activated kinasedependent mechanism, stimulating Egfr and Cox-2 activities and PGE 2 production (82)(83)(84)(85). Also, other factors may enhance Egfr activity in the Min/ϩ small intestine independent of PGE 2 .…”

Section: Discussionmentioning

confidence: 99%

Apc Deficiency Is Associated with Increased Egfr Activity in the Intestinal Enterocytes and Adenomas of C57BL/6J-Min/+ Mice

Moran¹,

Hunt

Javid³

et al. 2004

Journal of Biological Chemistry

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Activation of cytosolic phospholipase A2 in Her14 fibroblasts by hydrogen peroxide: a p42/44MAPK-dependent and phosphorylation-independent mechanism

Cited by 45 publications

References 87 publications

Oxidative Stress ActivatesFUS1andRLM1Transcription in the YeastSaccharomyces cerevisiaein an Oxidant-dependent Manner

Oxidative Stress ActivatesFUS1andRLM1Transcription in the YeastSaccharomyces cerevisiaein an Oxidant-dependent Manner

Immediate Antioxidant and Antiplatelet Effect of Atorvastatin via Inhibition of Nox2

Apc Deficiency Is Associated with Increased Egfr Activity in the Intestinal Enterocytes and Adenomas of C57BL/6J-Min/+ Mice

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