Activation of ɛ protein kinase C correlates with a cardioprotective effect of regular ethanol consumption

Abstract

Medical Sciences. In the article "Activation of protein kinase C correlates with a cardioprotective effect of regular ethanol consumption" by Masami Miyamae, Manuel M. Rodriguez, S.

Ethanol Regulation of D1 Dopamine Receptor Signaling is Mediated by Protein Kinase C in an Isozyme-Specific Manner

“…Chronic EtOH exposure (48 h) promotes translocation of PKCd and PKCe between specific intracellular compartments (Gordon et al, 1997). The subcellular redistribution of PKCe but not PKCd is also evident in cellular fractions prepared from cardiac myocytes (Miyamae et al, 1998). Moreover, acute EtOH administration differentially alters PKC expression in membrane and cytosolic fractions prepared from the rat cortex.…”

Sustained in vivo cardiac protection by a rationally designed peptide that causes ɛ protein kinase C translocation

“…PKC activation has been described previously in forms of preconditioning induced by ischemia or exposure to low amounts of ethanol (4,8,14). The studies in RACK-containing cardiomyocytes and transgenic mouse hearts demonstrate that even modest activation of Fig.…”

“…Because recent studies have suggested that translocation of PKC is correlated with ischemic preconditioning in adult cardiomyocytes (8,9,14), we determined whether translocation of PKC by RACK is sufficient to confer this protection. RACK (0.5 M extracellular concentration) reduced adult cardiac myocyte damage, as assessed by osmotic fragility, after 180 min of simulated ischemia from 37 Ϯ 5 to 16 Ϯ 4%, respectively (n ϭ 3; P Ͻ 0.05) ( Fig.…”

“…Studies in other systems correlated PKC activation with cardio-protection but did not directly demonstrate its role in the process (8,14). If activation of PKC is indeed required for cardio-protection, an PKCselective peptide agonist would be expected to act as a preconditioning mimetic.…”

Alcohol and the heart: To abstain or not to abstain?

“…In an animal models, longterm alcohol consumption reduced myocardial ischemia-reperfusion injury possibly through repeated myocyte adenosine receptor activation, [119] and sustained translocation of epsilon protein kinase C (εPKC). [120] In situ studies in rabbit showed, ethanol exposure followed by washout or sufficient time to metabolize the alcohol prior to ischemia, induces preconditioning-like myocardial protection. [121] Protection afforded against ischemia and reperfusion injury (I/R) proceeds through an ischemic preconditioning-like mechanism involving the activation of εPKC that is dependent on the time and duration of ethanol treatment.…”