2014
DOI: 10.1093/molehr/gau020
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Activation of endocrine-related gene expression in placental choriocarcinoma cell lines following DNA methylation knock-down

Abstract: Increasingly, placental DNA methylation is assessed as a factor in pregnancy-related complications, yet the transcriptional impact of such findings is not always clear. Using a proliferative in vitro placental model, the effect of DNA methylation loss on gene activation was evaluated at a number of genes selected for being differentially methylated in pre-eclampsia-associated placentae in vivo. We aimed to determine whether reduced DNA methylation at specific loci was associated with transcriptional changes at… Show more

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Cited by 23 publications
(19 citation statements)
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“…Human CYP19A1 has at least eight different transcriptional start sites encoding unique upstream exons, each of which is specific to a different tissue. Using this method, DNA methylation was found to regulate aromatase expression in primary APs, 75 breast adipose fibroblasts, 76 skin fibroblasts, 77 endometrial stromal cells, 78 placental choriocarcinoma, 79 placenta, 80 and neuroglia. 71,72 A large body of work demonstrates that epigenetic regulation of these promoters is important in modulating the expression of aromatase.…”
Section: The Role Of Estrogens In the Microenvironment And Their Inflmentioning
confidence: 99%
See 1 more Smart Citation
“…Human CYP19A1 has at least eight different transcriptional start sites encoding unique upstream exons, each of which is specific to a different tissue. Using this method, DNA methylation was found to regulate aromatase expression in primary APs, 75 breast adipose fibroblasts, 76 skin fibroblasts, 77 endometrial stromal cells, 78 placental choriocarcinoma, 79 placenta, 80 and neuroglia. 71,72 A large body of work demonstrates that epigenetic regulation of these promoters is important in modulating the expression of aromatase.…”
Section: The Role Of Estrogens In the Microenvironment And Their Inflmentioning
confidence: 99%
“…DNA methylation-the addition of a methyl group to the 5-position of cytosine-is a well-studied epigenetic mechanism, 73 and can be modulated by treatment of cells with the DNA methylation inhibitors 5-azacytidine or 2-deoxy-5-azacytidine 74 to test whether a gene is under epigenetic control. Using this method, DNA methylation was found to regulate aromatase expression in primary APs, 75 breast adipose fibroblasts, 76 skin fibroblasts, 77 endometrial stromal cells, 78 placental choriocarcinoma, 79 placenta, 80 and neuroglia. 81 Epigenetic regulation of aromatase by DNA methylation appears widely conserved across evolution, including in chicken, 82 rat, 83 alligator, 84 sea bass, 85 zebrafish, 86 perch, 87 eel, 88 buffalo, 89,90 sheep, and cattle.…”
Section: The Role Of Estrogens In the Microenvironment And Their Inflmentioning
confidence: 99%
“…For example, methylation and down-regulation of OCT4 is associated with normal trophoblast differentiation early in development (Zhang et al 2008) and methylation of tumor suppressor genes, such as RASSF1 or APC, is associated with trophoblast invasiveness (Novakovic et al 2008). Further evidence for the importance of DNA methylation for gene expression in placental trophoblast comes from in vitro studies using 5-Aza-2 0 -deoxycytidine to inhibit DNA methylation in choriocarcinoma cells (Hogg et al 2014b). …”
Section: Gene Promoter Methylationmentioning
confidence: 99%
“…PCR products were then pyrosequenced using a PyroMark Q96 ID System (Qiagen, United States). PCR primers used to detect LINE-1 methylation level as described previously (Braiteh et al, 2008;Hogg et al, 2014) were: Forward, TTTTGAGTTAGGTGTGGGATATA; Reverse, Bio-AAAATCAAAAAATTCCCTTTC; sequencing primer, AGTTAGGTGTGGGATATAGT. Primer sequences and sequences of rDNA promoter regions whose methylation levels were analyzed are presented in Table 1 and Supplementary Figure S1.…”
Section: Dna Isolation Genotyping and Pyrosequencingmentioning
confidence: 99%