Activation of galactose utilization by the addition of glucose for the fermentation of agar hydrolysate using Lactobacillus brevis ATCC 14869

Mwiti, Godfrey; Yeo, In‐Seok; Jeong, Kwan; Choi, Hyung-Seok; Kim, Jae-Han

doi:10.1007/s10529-022-03267-6

Cited by 2 publications

(3 citation statements)

References 25 publications

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“…Fermentation of marine biomass in bioreactors reported in literature are shown in Table 9. Results of fermentation of agar by Lactobacillus brevis reached a final product of 31.9 g/L of lactic acid with 17.2 g/L of ethanol as a byproduct [53]. In this case, glucose was required to activate galactose consumption by L. brevis, which was not necessary when fermenting K. alvarezii hydrolysates that already contain a low quantity of glucose.…”

Section: Fermentability Of K Alvarezii Hydrolysates By L Pentosusmentioning

confidence: 94%

“…Jang et al [49] executed fermentation in a 1-L working volume bioreactor reaching 14.5 g/L of lactic acid, with a 0.9 (g/g) yield. A study of the potential for using marine biomass in bioreactor fermentation was done by Mwiti et al [53], where hydrolysates of commercial agar were fermented in a 2-L working volume fed batch reactor, in this case, reaching 31.9 g/L of lactic acid.…”

Section: Introductionmentioning

confidence: 99%

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Third Generation Lactic Acid Production by Lactobacillus pentosus from the Macroalgae Kappaphycus alvarezii Hydrolysates

2023

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The evaluation of macroalgae as a new raw material for diverse bioprocesses is of great interest due to their fast growth rate and low environmental impact. Lactic acid has a high value in the bio-based industry and is mainly produced via fermentation. The anaerobic lactic acid fermentation of Kappaphycus alvarezii hydrolysates using the high-producing strain Lactobacillus pentosus was evaluated for detoxified and non-treated hydrolysates prepared from concentrated algal biomass and dilute acid solution mixtures. A novel hydrolysate detoxification procedure, combining activated charcoal and over-liming, for 5-hydroxymethylfurfural (HMF) removal was used. L. pentosus was found to successfully ferment detoxified and untreated hydrolysates produced in up to 30% and 20% w/v solutions, respectively. Significant production rates (1.88 g/L.h) and short lag phases were achieved in bioreactor fermentation operating at 37 °C and pH 6 with 150 rpm impeller velocity. A 0.94 g/g yield from fermentable sugars (galactose and glucose) was achieved, indicating that K. alvarezii could be used as a raw material for lactic acid production, within the context of Third Generation (3G) biorefinery.

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Section: Fermentability Of K Alvarezii Hydrolysates By L Pentosusmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Third Generation Lactic Acid Production by Lactobacillus pentosus from the Macroalgae Kappaphycus alvarezii Hydrolysates

2023

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“…Jang et al [36] evaluated hydrolysates brown seaweed, using Laminaria japonica hydrolysate on a bioreactor scale, reaching 14.4 g/L of lactic acid. Mwiti et al [37] fermented galactose derived from agar hydrolysate in a pulse fed-batch reactor resulting in 31.9 g/L of lactic acid. In a recent study [14], batch bioreactor fermentation of K. alvarezii hydrolysates reached 29.4 g/L.…”

Section: Introductionmentioning

confidence: 99%

Lactic Acid Fermentation of Carrageenan Hydrolysates from the Macroalga Kappaphycus alvarezii: Evaluating Different Bioreactor Operation Modes

Tabacof,

Calado,

Pereira

2023

Polysaccharides

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Lactic acid is a molecule used abundantly in the food, cosmetic, and pharmaceutical industries. It is also the building block for polylactic acid, a biodegradable polymer which has gained interest over the last decade. Seaweeds are fast growing, environmentally friendly, and economically beneficial. The Rhodophyta, Kappaphycus alvarezii, is a carrageenan-rich alga, which can be successfully fermented into lactic acid using lactic acid bacteria. Lactobacillus pentosus is a versatile and robust bacterium and an efficient producer of lactic acid from many different raw materials. Bioreactor strategies for lactic acid fermentation of K. alvarezii hydrolysate were tested in 2-L stirred-tank bioreactor fermentations, operating at 37 °C, pH 6, and 150 rpm. Productivity and yields were 1.37 g/(L.h) and 1.17 g/g for the pulse fed-batch, and 1.10 g/(L.h) and 1.04 g/g for extended fed-batch systems. A 3.57 g/(L.h) production rate and a 1.37 g/g yield for batch fermentation operating with an inoculum size of 0.6 g/L was recorded. When applying fed-batch strategies, fermentation products reached 91 g/L with pulse feed and 133 g/L with constant continuous feed. For control and comparison, a simple batch of synthetic galactose-rich Man-Sharpe-Rugosa (MRS) media was fermented at the same conditions. A short study of charcoal regenerability is shown. A scheme for a third-generation lactic acid biorefinery is proposed, envisioning a future sustainable large-scale production of this important organic acid.

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Activation of galactose utilization by the addition of glucose for the fermentation of agar hydrolysate using Lactobacillus brevis ATCC 14869

Cited by 2 publications

References 25 publications

Third Generation Lactic Acid Production by Lactobacillus pentosus from the Macroalgae Kappaphycus alvarezii Hydrolysates

Third Generation Lactic Acid Production by Lactobacillus pentosus from the Macroalgae Kappaphycus alvarezii Hydrolysates

Lactic Acid Fermentation of Carrageenan Hydrolysates from the Macroalga Kappaphycus alvarezii: Evaluating Different Bioreactor Operation Modes

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